Lupton SD, Brunton LL, Kalberg VA, Overell RW (1991)
Dominant positive and negative selection using a
hygromycin phosphotransferase-thymidine kinase
fusion gene. Mol Cell Biol 11:3374–3378
Ma Y, Zhang J, Yin W, Zhang Z, Song Y, Chang X (2016)
Targeted AID-mediated mutagenesis (TAM)
enables efficient genomic diversification in mammalian cells. Nat Methods 13:1029–1035
Maruyama J, Nakajima H, Kitamoto K (2001) Visualization of nuclei in Aspergillus oryzae with EGFP
and analysis of the number of nuclei in each conidium by FACS. Biosci Biotechnol Biochem
65:1504–1510
Marx F, Haas H, Reindl M, Sto ¨ffler G, Lottspeich F,
Redl B (1995) Cloning, structural organization
and regulation of expression of the Penicillium
chrysogenum paf gene encoding an abundantly
secreted protein with antifungal activity. Gene
167:167–171
Masai K, Marumaya J, Nakajima H, Kitamoto K (2003)
In vivo visualization of the distribution of a secretory protein in Aspergillus oryzae hyphae using the
RntA-EGFP fusion protein. Biosci Biotechnol Biochem 67:455–459
Matsuda Y, Mitsuhashi T, Lee S, Hoshino M, Mori T,
Okada M, Zhang H, Hayashi F, Fujita M, Abe I
(2016) Astellifadiene: structure determination by
NMR spectroscopy and crystalline sponge method,
and elucidation of its biosynthesis. Angew Chem
128:5879–5882
McNally MT, Free SJ (1988) Isolation and characterization of a Neurospora glucose-repressible gene.
Curr Genet 14:545–551
Meyer V, Arentshorst M, El-Ghezal A, Drews AC,
Kooistra R, van den Hondel CAMJJ, Ram AFJ
(2007) Highly efficient gene targeting in the Aspergillus niger kusA mutant. J Biotechnol 128:770–775
Meyer V, Wanka F, van Gent J, Arentshorst M, van den
Hondel CAMJJ, Ram AFJ (2011) Fungal gene
expression on demand: an inducible, tunable,
and metabolism-independent expression system
for Aspergillus niger. Appl Environ Microbiol
77:2975–2983
Meyer V, Andersen MR, Brakhage AA, Braus GH, Caddick MX, Cairns TC, de Vries RP, Haarmann T,
Hansen K, Hertz-Fowler C, Krappmann S, Mortensen UH, Pen ˜alva MA, Ram AFJ, Head RM (2016)
Current challenges of research on filamentous
fungi in relation to human welfare and a sustainable bio-economy: a white paper. Fungal Biol Biotechnol 3:1–17
Miyauchi S, Te’o VS, Bergquist PL, Nevalainen KMH
(2013) Expression of a bacterial xylanase in Trichoderma reesei under the egl2 and cbh2 glycosyl
hydrolase gene promoters. New Biotechnol
30:523–530
Moralejo FJ, Watson AJ, Jeenes DJ, Archer DB, Martı ´n
JF (2001) A defined level of protein disulfide isomerase expression is required for optimal secretion
of thaumatin by Aspergillus awamori. Mol Gen
Genomics 266:246–253
Morris MC (2010) Fluorescent biosensors of intracellular targets from genetically encoded reporters to
modular polypeptide probes. Cell Biochem Biophys 56:19–37
Morse NJ, Gopal MR, Wagner JM, Alper HS (2017)
Yeast terminator function can be modulated and
designed on the basis of predictions of nucleosome
occupancy. ACS Synth Biol 6:2086–2095
Muller C, Hjort CM, Hansen K, Nielsen J (2002) Altering the expression of two chitin synthase genes
differentially affects the growth and morphology
of Aspergillus oryzae. Microbiology 148:4025–4033
Munawar A, Marshall JW, Cox RJ, Bailey AM, Lazarus
CM (2013) Isolation and characterisation of a
ferrirhodin synthetase gene from the sugarcane
pathogen Fusarium sacchari. Chembiochem
14:388–394
Nagamine S, Liu C, Nishishita J, Kozaki T, Sogahata K,
Sato Y, Minami A, Ozaki T, Schmidt-Dannert C,
Maruyama J, Oikawa H (2019) Ascomycota Aspergillus oryzae is an efficient expression host for
production of basidiomycota terpenes using genomic DNA sequences. Appl Environ Microbiol
85:1–21
Nakajima KI, Asakura T, Maruyama JI, Morita Y, Oike
H, Shimizu-Ibuka A, Misaka T, Sorimachi H, Arai
S, Kitamoto K, Abe K (2006) Extracellular production of neoculin, a sweet-tasting heterodimeric
protein with taste-modifying activity, by Aspergillus oryzae. Appl Environ Microbiol 72:3716–3723
Nakano ET, Fox RD, Clements DE, Koo K, Stuart WD,
Ivy JM (1993) Expression vectors for Neurospora
crassa and expression of a bovine preprochymosin
cDNA. Fungal Genet Rep 40:54–56
Nakari-Seta ¨la ¨ T, Penttila ¨ M (1995) Production of Trichoderma reesei cellulases glucose-containing
media. Appl Environ Microbiol 61:3650–3655
Nakazato L, Dutra V, Broetto L, Staats CC, Vainstein
MH, Schrank A (2006) Development of an expression vector for Metarhizium anisopliae based on
the tef-1a homologous promoter. Appl Microbiol
Biotechnol 72:521–528
Nielsen ML, Albertsen L, Lettier G, Nielsen JB, Mortensen UH (2006) Efficient PCR-based gene targeting
with a recyclable marker for Aspergillus nidulans.
Fungal Genet Biol 43:54–64
Nielsen MT, Nielsen JB, Anyaogu DC, Holm DK, Nielsen KF, Larsen TO, Mortensen UH (2013) Heterologous reconstitution of the intact geodin gene
cluster in Aspergillus nidulans through a simple
and versatile PCR based approach. PLoS One 8:
e72871
Nielsen ML, Isbrandt T, Rasmussen KB, Thrane U,
Hoof JB, Larsen TO, Mortensen UH (2017) Genes
linked to production of secondary metabolites in
Talaromyces atroroseus revealed using CRISPRCas9. PLoS One 12:1–9
Nødvig CS, Nielsen JB, Kogle ME, Mortensen UH
(2015) A CRISPR-Cas9 system for genetic engineering of filamentous fungi. PLoS One 10:1–18
10 Filamentous Fungi as Hosts for Heterologous Production of Proteins and Secondary. . .
261
Dominant positive and negative selection using a
hygromycin phosphotransferase-thymidine kinase
fusion gene. Mol Cell Biol 11:3374–3378
Ma Y, Zhang J, Yin W, Zhang Z, Song Y, Chang X (2016)
Targeted AID-mediated mutagenesis (TAM)
enables efficient genomic diversification in mammalian cells. Nat Methods 13:1029–1035
Maruyama J, Nakajima H, Kitamoto K (2001) Visualization of nuclei in Aspergillus oryzae with EGFP
and analysis of the number of nuclei in each conidium by FACS. Biosci Biotechnol Biochem
65:1504–1510
Marx F, Haas H, Reindl M, Sto ¨ffler G, Lottspeich F,
Redl B (1995) Cloning, structural organization
and regulation of expression of the Penicillium
chrysogenum paf gene encoding an abundantly
secreted protein with antifungal activity. Gene
167:167–171
Masai K, Marumaya J, Nakajima H, Kitamoto K (2003)
In vivo visualization of the distribution of a secretory protein in Aspergillus oryzae hyphae using the
RntA-EGFP fusion protein. Biosci Biotechnol Biochem 67:455–459
Matsuda Y, Mitsuhashi T, Lee S, Hoshino M, Mori T,
Okada M, Zhang H, Hayashi F, Fujita M, Abe I
(2016) Astellifadiene: structure determination by
NMR spectroscopy and crystalline sponge method,
and elucidation of its biosynthesis. Angew Chem
128:5879–5882
McNally MT, Free SJ (1988) Isolation and characterization of a Neurospora glucose-repressible gene.
Curr Genet 14:545–551
Meyer V, Arentshorst M, El-Ghezal A, Drews AC,
Kooistra R, van den Hondel CAMJJ, Ram AFJ
(2007) Highly efficient gene targeting in the Aspergillus niger kusA mutant. J Biotechnol 128:770–775
Meyer V, Wanka F, van Gent J, Arentshorst M, van den
Hondel CAMJJ, Ram AFJ (2011) Fungal gene
expression on demand: an inducible, tunable,
and metabolism-independent expression system
for Aspergillus niger. Appl Environ Microbiol
77:2975–2983
Meyer V, Andersen MR, Brakhage AA, Braus GH, Caddick MX, Cairns TC, de Vries RP, Haarmann T,
Hansen K, Hertz-Fowler C, Krappmann S, Mortensen UH, Pen ˜alva MA, Ram AFJ, Head RM (2016)
Current challenges of research on filamentous
fungi in relation to human welfare and a sustainable bio-economy: a white paper. Fungal Biol Biotechnol 3:1–17
Miyauchi S, Te’o VS, Bergquist PL, Nevalainen KMH
(2013) Expression of a bacterial xylanase in Trichoderma reesei under the egl2 and cbh2 glycosyl
hydrolase gene promoters. New Biotechnol
30:523–530
Moralejo FJ, Watson AJ, Jeenes DJ, Archer DB, Martı ´n
JF (2001) A defined level of protein disulfide isomerase expression is required for optimal secretion
of thaumatin by Aspergillus awamori. Mol Gen
Genomics 266:246–253
Morris MC (2010) Fluorescent biosensors of intracellular targets from genetically encoded reporters to
modular polypeptide probes. Cell Biochem Biophys 56:19–37
Morse NJ, Gopal MR, Wagner JM, Alper HS (2017)
Yeast terminator function can be modulated and
designed on the basis of predictions of nucleosome
occupancy. ACS Synth Biol 6:2086–2095
Muller C, Hjort CM, Hansen K, Nielsen J (2002) Altering the expression of two chitin synthase genes
differentially affects the growth and morphology
of Aspergillus oryzae. Microbiology 148:4025–4033
Munawar A, Marshall JW, Cox RJ, Bailey AM, Lazarus
CM (2013) Isolation and characterisation of a
ferrirhodin synthetase gene from the sugarcane
pathogen Fusarium sacchari. Chembiochem
14:388–394
Nagamine S, Liu C, Nishishita J, Kozaki T, Sogahata K,
Sato Y, Minami A, Ozaki T, Schmidt-Dannert C,
Maruyama J, Oikawa H (2019) Ascomycota Aspergillus oryzae is an efficient expression host for
production of basidiomycota terpenes using genomic DNA sequences. Appl Environ Microbiol
85:1–21
Nakajima KI, Asakura T, Maruyama JI, Morita Y, Oike
H, Shimizu-Ibuka A, Misaka T, Sorimachi H, Arai
S, Kitamoto K, Abe K (2006) Extracellular production of neoculin, a sweet-tasting heterodimeric
protein with taste-modifying activity, by Aspergillus oryzae. Appl Environ Microbiol 72:3716–3723
Nakano ET, Fox RD, Clements DE, Koo K, Stuart WD,
Ivy JM (1993) Expression vectors for Neurospora
crassa and expression of a bovine preprochymosin
cDNA. Fungal Genet Rep 40:54–56
Nakari-Seta ¨la ¨ T, Penttila ¨ M (1995) Production of Trichoderma reesei cellulases glucose-containing
media. Appl Environ Microbiol 61:3650–3655
Nakazato L, Dutra V, Broetto L, Staats CC, Vainstein
MH, Schrank A (2006) Development of an expression vector for Metarhizium anisopliae based on
the tef-1a homologous promoter. Appl Microbiol
Biotechnol 72:521–528
Nielsen ML, Albertsen L, Lettier G, Nielsen JB, Mortensen UH (2006) Efficient PCR-based gene targeting
with a recyclable marker for Aspergillus nidulans.
Fungal Genet Biol 43:54–64
Nielsen MT, Nielsen JB, Anyaogu DC, Holm DK, Nielsen KF, Larsen TO, Mortensen UH (2013) Heterologous reconstitution of the intact geodin gene
cluster in Aspergillus nidulans through a simple
and versatile PCR based approach. PLoS One 8:
e72871
Nielsen ML, Isbrandt T, Rasmussen KB, Thrane U,
Hoof JB, Larsen TO, Mortensen UH (2017) Genes
linked to production of secondary metabolites in
Talaromyces atroroseus revealed using CRISPRCas9. PLoS One 12:1–9
Nødvig CS, Nielsen JB, Kogle ME, Mortensen UH
(2015) A CRISPR-Cas9 system for genetic engineering of filamentous fungi. PLoS One 10:1–18
10 Filamentous Fungi as Hosts for Heterologous Production of Proteins and Secondary. . .
261