zae and its expression in Saccharomyces cerevisiae
and Aspergillus oryzae. Biosci Biotechnol Biochem
59:1869–1874
Fujii I, Ono Y, Tada H, Gomi K, Ebizuka Y, Sankawa U
(1996) Cloning of the polyketide synthase gene atX
from Aspergillus terreus and its identification as
the 6-methylsalicylic acid synthase gene by heterologous expression. Mol Gen Genomics 253:1–10
Fujii I, Yoshida N, Shimomaki S, Oikawa H, Ebizuka Y
(2005) An iterative type I polyketide synthase
PKSN catalyzes synthesis of the decaketide alternapyrone with regio-specific octa-methylation.
Chem Biol 12:1301–1309
Gao F, Hao Z, Sun X, Qin L, Zhao T, Liu W, Luo H, Yao
B, Su X (2018) A versatile system for fast screening
and isolation of Trichoderma reesei cellulase
hyperproducers
based
on
DsRed
and
fluorescence-assisted cell sorting. Biotechnol Biofuels 11:1–13
Gardiner DM, Jarvis RS, Howlett BJ (2005) The ABC
transporter gene in the sirodesmin biosynthetic
gene cluster of Leptosphaeria maculans is not
essential for sirodesmin production but facilitates
self-protection. Fungal Genet Biol 42:257–263
Garneau-Tsodikova S, Labby KJ (2016) Mechanisms of
resistance to aminoglycoside antibiotics: overview
and perspectives. Medchemcomm 7:11–27
Gems D, Johnstone IL, Clutterbuck AJ (1991) An autonomously replicating plasmid transforms Aspergillus nidulans at high frequency. Gene 98:61–67
Gibson DG, Young L, Chuang RY, Craig Venter J,
Hutchison CA III, Smith HO (2009) Enzymatic
assembly of DNA molecules up to several hundred
kilobases. Nat Methods 6:343–347
Giles NH, Case ME, Baum J, Geever R, Huiet L, Patel V,
Tyler B (1985) Gene organization and regulation in
the qa (quinic acid) gene cluster of Neurospora
crassa. Microbiol Rev 49:338–358
Gomi K, Akeno T, Minetoki T, Ozeki K, Kumagai C,
Okazaki N, Iimura Y (2000) Molecular cloning and
characterization of a transcriptional activator
gene, amyR, involved in the amylolytic gene
expression in Aspergillus oryzae. Biosci Biotechnol
Biochem 64:816–827
Goosen T, Bloemheuvel G, Gysler C, de Bie DA, van den
Broek HWJ, Swart K (1987) Transformation of
Aspergillus niger using the homologous orotidine-5
0 -phosphate-decarboxylase gene. Curr
Genet 11:499–503
Goosen T, van Engelenburg F, Debets F, Swart K, Bos K,
van den Broek H (1989) Tryptophan auxotrophic
mutants in Aspergillus niger: inactivation of the
trpC gene by cotransformation mutagenesis. Mol
Gen Genet 219:282–288
Gossen M, Bujard H (1992) Tight control of gene
expression in mammalian cells by tetracyclineresponsive promoters. Proc Natl Acad Sci USA
89:5547–5551
Gossen M, Freundlieb S, Bender G, Mu ¨ller G, Hillen W,
Bujardt H (1995) Transcriptional activation by
tetracyclines in mammalian cells. Science
268:1766–1769
Gouka RJ, Punt PJ, Van Den Hondel CAMJJ (1997)
Glucoamylase gene fusions alleviate limitations
for protein production in Aspergillus awamori at
the transcriptional and (post)translational levels.
Appl Environ Microbiol 63:488–497
Gould N, Hendy O, Papamichail D (2014) Computational tools and algorithms for designing customized synthetic genes. Front Bioeng Biotechnol
2:1–14
Gressler M, Hortschansky P, Geib E, Brock M (2015) A
new high-performance heterologous fungal
expression system based on regulatory elements
from the Aspergillus terreus terrein gene cluster.
Front Microbiol 6:1–16
Grigoriev IV, Nikitin R, Haridas S, Kuo A, Ohm R,
Otillar R, Riley R, Salamov A, Zhao X, Korzeniewski F, Smirnova T, Nordberg H, Dubchak I,
Shabalov I (2014) MycoCosm portal: gearing up
for 1000 fungal genomes. Nucleic Acids Res 42:
D699–D704
Gupta SK, Shukla P (2018) Glycosylation control technologies for recombinant therapeutic proteins.
Appl Microbiol Biotechnol 102:10457–10468
Haas H, Redl B, Friedlin E, Sto ¨ffler G (1992) Isolation
and analysis of the Penicillium chrysogenum phoA
gene encoding a secreted phosphate-repressible
acid phosphatase. Gene 113:129–133
Halo LM, Marshall JW, Yakasai AA, Song Z, Butts CP,
Crump MP, Heneghan M, Bailey AM, Simpson TJ,
Lazarus CM, Cox RJ (2008) Authentic heterologous expression of the tenellin iterative polyketide
synthase nonribosomal peptide synthetase
requires coexpression with an enoyl reductase.
Chembiochem 9:585–594
Hansen BG, Genee HJ, Kaas CS, Nielsen JB, Regueira
TB, Mortensen UH, Frisvad JC, Patil KR (2011a) A
new class of IMP dehydrogenase with a role in selfresistance of mycophenolic acid producing fungi.
BMC Microbiol 11:1–7
Hansen BG, Salomonsen B, Nielsen MT, Nielsen JB,
Hansen NB, Nielsen KF, Regueira TB, Nielsen J,
Patil KR, Mortensen UH (2011b) Versatile enzyme
expression and characterization system for Aspergillus nidulans, with the Penicillium brevicompactum polyketide synthase gene from the
mycophenolic acid gene cluster as a test case.
Appl Environ Microbiol 77:3044–3051
Hanson G, Coller J (2018) Codon optimality, bias and
usage in translation and mRNA decay. Nat Rev
Mol Cell Biol 19:20–30
He Y, Cox RJ (2016) The molecular steps of citrinin
biosynthesis in fungi. Chem Sci 7:2119–2127
Herr A, Fischer R (2014) Improvement of Aspergillus
nidulans penicillin production by targeting AcvA
to peroxisomes. Metab Eng 25:131–139
258
J. K. H. Rendsvig et al.
and Aspergillus oryzae. Biosci Biotechnol Biochem
59:1869–1874
Fujii I, Ono Y, Tada H, Gomi K, Ebizuka Y, Sankawa U
(1996) Cloning of the polyketide synthase gene atX
from Aspergillus terreus and its identification as
the 6-methylsalicylic acid synthase gene by heterologous expression. Mol Gen Genomics 253:1–10
Fujii I, Yoshida N, Shimomaki S, Oikawa H, Ebizuka Y
(2005) An iterative type I polyketide synthase
PKSN catalyzes synthesis of the decaketide alternapyrone with regio-specific octa-methylation.
Chem Biol 12:1301–1309
Gao F, Hao Z, Sun X, Qin L, Zhao T, Liu W, Luo H, Yao
B, Su X (2018) A versatile system for fast screening
and isolation of Trichoderma reesei cellulase
hyperproducers
based
on
DsRed
and
fluorescence-assisted cell sorting. Biotechnol Biofuels 11:1–13
Gardiner DM, Jarvis RS, Howlett BJ (2005) The ABC
transporter gene in the sirodesmin biosynthetic
gene cluster of Leptosphaeria maculans is not
essential for sirodesmin production but facilitates
self-protection. Fungal Genet Biol 42:257–263
Garneau-Tsodikova S, Labby KJ (2016) Mechanisms of
resistance to aminoglycoside antibiotics: overview
and perspectives. Medchemcomm 7:11–27
Gems D, Johnstone IL, Clutterbuck AJ (1991) An autonomously replicating plasmid transforms Aspergillus nidulans at high frequency. Gene 98:61–67
Gibson DG, Young L, Chuang RY, Craig Venter J,
Hutchison CA III, Smith HO (2009) Enzymatic
assembly of DNA molecules up to several hundred
kilobases. Nat Methods 6:343–347
Giles NH, Case ME, Baum J, Geever R, Huiet L, Patel V,
Tyler B (1985) Gene organization and regulation in
the qa (quinic acid) gene cluster of Neurospora
crassa. Microbiol Rev 49:338–358
Gomi K, Akeno T, Minetoki T, Ozeki K, Kumagai C,
Okazaki N, Iimura Y (2000) Molecular cloning and
characterization of a transcriptional activator
gene, amyR, involved in the amylolytic gene
expression in Aspergillus oryzae. Biosci Biotechnol
Biochem 64:816–827
Goosen T, Bloemheuvel G, Gysler C, de Bie DA, van den
Broek HWJ, Swart K (1987) Transformation of
Aspergillus niger using the homologous orotidine-5
0 -phosphate-decarboxylase gene. Curr
Genet 11:499–503
Goosen T, van Engelenburg F, Debets F, Swart K, Bos K,
van den Broek H (1989) Tryptophan auxotrophic
mutants in Aspergillus niger: inactivation of the
trpC gene by cotransformation mutagenesis. Mol
Gen Genet 219:282–288
Gossen M, Bujard H (1992) Tight control of gene
expression in mammalian cells by tetracyclineresponsive promoters. Proc Natl Acad Sci USA
89:5547–5551
Gossen M, Freundlieb S, Bender G, Mu ¨ller G, Hillen W,
Bujardt H (1995) Transcriptional activation by
tetracyclines in mammalian cells. Science
268:1766–1769
Gouka RJ, Punt PJ, Van Den Hondel CAMJJ (1997)
Glucoamylase gene fusions alleviate limitations
for protein production in Aspergillus awamori at
the transcriptional and (post)translational levels.
Appl Environ Microbiol 63:488–497
Gould N, Hendy O, Papamichail D (2014) Computational tools and algorithms for designing customized synthetic genes. Front Bioeng Biotechnol
2:1–14
Gressler M, Hortschansky P, Geib E, Brock M (2015) A
new high-performance heterologous fungal
expression system based on regulatory elements
from the Aspergillus terreus terrein gene cluster.
Front Microbiol 6:1–16
Grigoriev IV, Nikitin R, Haridas S, Kuo A, Ohm R,
Otillar R, Riley R, Salamov A, Zhao X, Korzeniewski F, Smirnova T, Nordberg H, Dubchak I,
Shabalov I (2014) MycoCosm portal: gearing up
for 1000 fungal genomes. Nucleic Acids Res 42:
D699–D704
Gupta SK, Shukla P (2018) Glycosylation control technologies for recombinant therapeutic proteins.
Appl Microbiol Biotechnol 102:10457–10468
Haas H, Redl B, Friedlin E, Sto ¨ffler G (1992) Isolation
and analysis of the Penicillium chrysogenum phoA
gene encoding a secreted phosphate-repressible
acid phosphatase. Gene 113:129–133
Halo LM, Marshall JW, Yakasai AA, Song Z, Butts CP,
Crump MP, Heneghan M, Bailey AM, Simpson TJ,
Lazarus CM, Cox RJ (2008) Authentic heterologous expression of the tenellin iterative polyketide
synthase nonribosomal peptide synthetase
requires coexpression with an enoyl reductase.
Chembiochem 9:585–594
Hansen BG, Genee HJ, Kaas CS, Nielsen JB, Regueira
TB, Mortensen UH, Frisvad JC, Patil KR (2011a) A
new class of IMP dehydrogenase with a role in selfresistance of mycophenolic acid producing fungi.
BMC Microbiol 11:1–7
Hansen BG, Salomonsen B, Nielsen MT, Nielsen JB,
Hansen NB, Nielsen KF, Regueira TB, Nielsen J,
Patil KR, Mortensen UH (2011b) Versatile enzyme
expression and characterization system for Aspergillus nidulans, with the Penicillium brevicompactum polyketide synthase gene from the
mycophenolic acid gene cluster as a test case.
Appl Environ Microbiol 77:3044–3051
Hanson G, Coller J (2018) Codon optimality, bias and
usage in translation and mRNA decay. Nat Rev
Mol Cell Biol 19:20–30
He Y, Cox RJ (2016) The molecular steps of citrinin
biosynthesis in fungi. Chem Sci 7:2119–2127
Herr A, Fischer R (2014) Improvement of Aspergillus
nidulans penicillin production by targeting AcvA
to peroxisomes. Metab Eng 25:131–139
258
J. K. H. Rendsvig et al.