13. Microscope glass slide (L/W/T: 75 mm/25 mm/1 mm).
14. PDMS (polydimethylsiloxane) monomer and curing agent
(Sylgard 184, Dow Corning).
15. PMMA (polymethylmethacrylate) board (thickness: 2 mm).
2.2 Magnetic
Tweezers (MT)
1. Polyethylene glycol-silane (PEG-silane, MW 2000, Laysan Bio,
Inc.).
2. S1813 photoresist (Shipley).
3. LOR 2A photoresist (Shipley).
4. Permalloy sputtering target (Ni 0.81 Fe 0.19 ).
5. EVG 620 Aligner.
6. PECVD (Plasma Therm 790 PECVD).
7. Oxygen plasma (PTS Oxygen Plasma System).
8. Sputtering deposition tool (AJA Orion RF/DC).
9. Cells those enable linking with superparamagnetic beads (e.g.,
T-lymphocytes, express CD45
+ ).
10. Dextran-coated magnetic microbeads (1 μm diameter, StemCell Technologies, Cat. No. 19250).
11. Depletion cocktail (EasySep™ Human CD45 Depletion Kit,
Stem Cell Technologies, Cat. No. 18259).
12. Tetrameric antibody complexes targeting CD45 antigens.
13. Phosphate buffer solution (PBS).
14. Incubator.
15. Four orthogonal electromagnets (Magnetech OP-2025).
16. Solenoid.
17. Computer and LabVIEW (National Instruments, TX).
18. Optical microscope (Leica Microsystems, Wetzlar, Germany)
with a 20Â objective and a high-speed camera.
19. 3D NEP chip.
2.3 DEP Generator
1. Digital function generator (DS345, Stanford Research
Systems).
2. Amplifier (LM6172 operational amplifier, Texas Instruments).
3. Heat sink.
4. Microswitch (Omron).
5. Oscilloscope (Tektronix TDS 3034C).
6. Sucrose solution (conductivity: ~0.03 S/m
À1 ).
7. 3D NEP chip.
34
Lingqian Chang et al.
14. PDMS (polydimethylsiloxane) monomer and curing agent
(Sylgard 184, Dow Corning).
15. PMMA (polymethylmethacrylate) board (thickness: 2 mm).
2.2 Magnetic
Tweezers (MT)
1. Polyethylene glycol-silane (PEG-silane, MW 2000, Laysan Bio,
Inc.).
2. S1813 photoresist (Shipley).
3. LOR 2A photoresist (Shipley).
4. Permalloy sputtering target (Ni 0.81 Fe 0.19 ).
5. EVG 620 Aligner.
6. PECVD (Plasma Therm 790 PECVD).
7. Oxygen plasma (PTS Oxygen Plasma System).
8. Sputtering deposition tool (AJA Orion RF/DC).
9. Cells those enable linking with superparamagnetic beads (e.g.,
T-lymphocytes, express CD45
+ ).
10. Dextran-coated magnetic microbeads (1 μm diameter, StemCell Technologies, Cat. No. 19250).
11. Depletion cocktail (EasySep™ Human CD45 Depletion Kit,
Stem Cell Technologies, Cat. No. 18259).
12. Tetrameric antibody complexes targeting CD45 antigens.
13. Phosphate buffer solution (PBS).
14. Incubator.
15. Four orthogonal electromagnets (Magnetech OP-2025).
16. Solenoid.
17. Computer and LabVIEW (National Instruments, TX).
18. Optical microscope (Leica Microsystems, Wetzlar, Germany)
with a 20Â objective and a high-speed camera.
19. 3D NEP chip.
2.3 DEP Generator
1. Digital function generator (DS345, Stanford Research
Systems).
2. Amplifier (LM6172 operational amplifier, Texas Instruments).
3. Heat sink.
4. Microswitch (Omron).
5. Oscilloscope (Tektronix TDS 3034C).
6. Sucrose solution (conductivity: ~0.03 S/m
À1 ).
7. 3D NEP chip.
34
Lingqian Chang et al.