The electric field strength is different on two sides of a single
cell positioned on the nanochannel, thus allowing an asymmetric
electric field to generate DEP forces to move the cell either toward
or away from the nanochannels. The direction of DEP force with
respect to electric field gradient was determined by Clausius–Mossotti equation. The schematic of 3D NEP–DEP system is shown in
Fig. 2b. A low conductive isotonic buffer solution [15] is used to
Fig. 2 Three high-throughput on-chip cellular manipulation techniques functionalized on 3D NEP device. (a)
Magnetic tweezers system, which precisely control magnetic field near the surface of the 3D NEP chip at X, Y,
Z direction by programmable magnets and solenoid. Reproduced with permission from Wiley. (b) Dielectrophoresis is realized by apply high frequency AC field (e.g., 100 kHz) on the same system setup of 3D NEP, in
order to capture cells toward nanochannel array. Reproduced with permission from RSC. (c) Thin film
microfluidics is a novel method for simple and efficient cell capture with three steps, including (i) pattern
microcaps around nanochannel on 3D NEP chip; (ii) chip is vertically dipped into cell buffer solution and lifted
up(dewetting procedure); (iii) cells hooked by the microcaps will be localized on the nanochannel. Reproduced
with permission from Wiley
32
Lingqian Chang et al.
cell positioned on the nanochannel, thus allowing an asymmetric
electric field to generate DEP forces to move the cell either toward
or away from the nanochannels. The direction of DEP force with
respect to electric field gradient was determined by Clausius–Mossotti equation. The schematic of 3D NEP–DEP system is shown in
Fig. 2b. A low conductive isotonic buffer solution [15] is used to
Fig. 2 Three high-throughput on-chip cellular manipulation techniques functionalized on 3D NEP device. (a)
Magnetic tweezers system, which precisely control magnetic field near the surface of the 3D NEP chip at X, Y,
Z direction by programmable magnets and solenoid. Reproduced with permission from Wiley. (b) Dielectrophoresis is realized by apply high frequency AC field (e.g., 100 kHz) on the same system setup of 3D NEP, in
order to capture cells toward nanochannel array. Reproduced with permission from RSC. (c) Thin film
microfluidics is a novel method for simple and efficient cell capture with three steps, including (i) pattern
microcaps around nanochannel on 3D NEP chip; (ii) chip is vertically dipped into cell buffer solution and lifted
up(dewetting procedure); (iii) cells hooked by the microcaps will be localized on the nanochannel. Reproduced
with permission from Wiley
32
Lingqian Chang et al.