11. Hypertonic environment is unfavorable to cell recovery and
needs to be removed. However, the cells after electric shock are
fragile and unsuitable for centrifugation. Therefore, 200 μL
BBM medium containing 1 g/L glucose was added into the
electric shock cuvette directly after electric shock, and then the
algal cells were centrifuged and collected and suspended in the
BBM medium containing 1 g/L glucose cultured at 30
C for
24 h without light.
References
1. Blanc G, Duncan G, Agarkova I,
Borodovsky M, Gurnon J, Kuo A,
Lindquist E, Lucas S, Pangilinan J, Polle J,
Salamov A, Terry A, Yamada T, Dunigan DD,
Grigoriev IV, Claverie JM, Van Etten JL
(2010) The Chlorella variabilis NC64A
genome reveals adaptation to photosymbiosis,
coevolution with viruses, and cryptic sex. Plant
Cell 22(9):2943–2955
2. Fan J, Ning K, Zeng X, Luo Y, Wang D, Hu J,
Li J, Xu H, Huang J, Wan M, Wang W,
Zhang D, Shen G, Run C, Liao J, Fang L,
Huang S, Jing X, Su X, Wang A, Bai L, Hu Z,
Xu J, Li Y (2015) Genomic foundation of
starch-to-lipid switch in oleaginous Chlorella
spp. Plant Physiol 169(4):2444–2461
3. Chungjatupornchai
W,
Kitraksa
P,
Fa-aroonsawat S (2016) Stable nuclear transformation of the oleaginous microalga Neochloris oleoabundans by electroporation. J
Appl Phycol 28(1):191–199
4. Gan QH, Jiang JY, Han X, Wang SF, Lu YD
(2018) Engineering the chloroplast genome of
oleaginous marine microalga Nannochloropsis
oceanica. Front Plant Sci 9:439
5. Niu YF, Yang ZK, Zhang MH, Zhu CC, Yang
WD, Liu JS, Li HY (2012) Transformation of
diatom Phaeodactylum tricornutum by electroporation and establishment of inducible
selection marker. BioTechniques 52(6)
6. Chow KC, Tung WL (1999) Electrotransformation of Chlorella vulgaris. Plant Cell Rep 18
(9):778–780
7. Run CL, Fang L, Fan JH, Fan CM, Luo YC,
Hu ZM, Li YG (2016) Stable nuclear transformation of the industrial alga Chlorella pyrenoidosa. Algal Res 17:196–201
8. Wang CH, Wang YY, Su Q, Gao XR (2007)
Transient expression of the GUS gene in a
unicellular marine green alga, Chlorella sp
MACC/C95, via electroporation. Biotechnol
Bioprocess Eng 12(2):180–183
9. Brown LE, Sprecher SL, Keller LR (1991)
Introduction of exogenous DNA into Chlamydomonas reinhardtii by electroporation. Mol
Cell Biol 11(4):2328–2332
10. Shimogawara K, Fujiwara S, Grossman A,
Usuda H (1998) High-efficiency transformation of Chlamydomonas reinhardtii by electroporation. Genetics 148(4):1821–1828
Electrotransformation of Chlorella Species
185
needs to be removed. However, the cells after electric shock are
fragile and unsuitable for centrifugation. Therefore, 200 μL
BBM medium containing 1 g/L glucose was added into the
electric shock cuvette directly after electric shock, and then the
algal cells were centrifuged and collected and suspended in the
BBM medium containing 1 g/L glucose cultured at 30
C for
24 h without light.
References
1. Blanc G, Duncan G, Agarkova I,
Borodovsky M, Gurnon J, Kuo A,
Lindquist E, Lucas S, Pangilinan J, Polle J,
Salamov A, Terry A, Yamada T, Dunigan DD,
Grigoriev IV, Claverie JM, Van Etten JL
(2010) The Chlorella variabilis NC64A
genome reveals adaptation to photosymbiosis,
coevolution with viruses, and cryptic sex. Plant
Cell 22(9):2943–2955
2. Fan J, Ning K, Zeng X, Luo Y, Wang D, Hu J,
Li J, Xu H, Huang J, Wan M, Wang W,
Zhang D, Shen G, Run C, Liao J, Fang L,
Huang S, Jing X, Su X, Wang A, Bai L, Hu Z,
Xu J, Li Y (2015) Genomic foundation of
starch-to-lipid switch in oleaginous Chlorella
spp. Plant Physiol 169(4):2444–2461
3. Chungjatupornchai
W,
Kitraksa
P,
Fa-aroonsawat S (2016) Stable nuclear transformation of the oleaginous microalga Neochloris oleoabundans by electroporation. J
Appl Phycol 28(1):191–199
4. Gan QH, Jiang JY, Han X, Wang SF, Lu YD
(2018) Engineering the chloroplast genome of
oleaginous marine microalga Nannochloropsis
oceanica. Front Plant Sci 9:439
5. Niu YF, Yang ZK, Zhang MH, Zhu CC, Yang
WD, Liu JS, Li HY (2012) Transformation of
diatom Phaeodactylum tricornutum by electroporation and establishment of inducible
selection marker. BioTechniques 52(6)
6. Chow KC, Tung WL (1999) Electrotransformation of Chlorella vulgaris. Plant Cell Rep 18
(9):778–780
7. Run CL, Fang L, Fan JH, Fan CM, Luo YC,
Hu ZM, Li YG (2016) Stable nuclear transformation of the industrial alga Chlorella pyrenoidosa. Algal Res 17:196–201
8. Wang CH, Wang YY, Su Q, Gao XR (2007)
Transient expression of the GUS gene in a
unicellular marine green alga, Chlorella sp
MACC/C95, via electroporation. Biotechnol
Bioprocess Eng 12(2):180–183
9. Brown LE, Sprecher SL, Keller LR (1991)
Introduction of exogenous DNA into Chlamydomonas reinhardtii by electroporation. Mol
Cell Biol 11(4):2328–2332
10. Shimogawara K, Fujiwara S, Grossman A,
Usuda H (1998) High-efficiency transformation of Chlamydomonas reinhardtii by electroporation. Genetics 148(4):1821–1828
Electrotransformation of Chlorella Species
185