Acknowledgments
This work was supported by the Japan Science and Technology
Agency, Advanced Low Carbon Technology Research and Development Program to K.I. (JPMJAL1105).
References
1. Falkowski PG, Barber RT, Smetacek VV
(1998) Biogeochemical controls and feedbacks
on ocean primary production. Science
281:200–207
2. Prihoda J, Tanaka A, de Paula WB, Allen JF,
Tirichine L, Bowler C (2012) Chloroplastmitochondria cross-talk in diatoms. J Exp Bot
63:1543–1557
3. Levitan O, Dinamarca J, Hochman G, Falkowski PG (2014) Diatoms: a fossil fuel of the
future. Trends Biotechnol 32:117–124
4. Wang Y, Cai J, Jiang Y, Jiang X, Zhang D
(2013) Preparation of biosilica structures
from frustules of diatoms and their applications: current state and perspectives. Appl
Microbiol Biotechnol 97:453–460
5. Zaslavskaia LA, Lippmeier JC, Kroth PG,
Grossman AR, Apt KE (2000) Transformation
of the diatom Phaeodactylum tricornutum
(Bacillariophyceae) with a variety of selectable
marker and reporter genes. J Phycol
36:379–386
6. Poulsen N, Chesley PM, Kroger N (2006)
Molecular genetic manipulation of the diatom
Thalassiosira pseudonana (Bacillariophyceae). J
Phycol 42:1059–1065
7. Miyagawa-Yamaguchi A, Okami T, Kira N,
Yamaguchi H, Ohnishi K, Adachi M (2011)
Stable nuclear transformation of the diatom
Chaetoceros sp. Phycol Res 59:113–119
8. Miyahara M, Aoi M, Inoue-Kashino N,
Kashino Y, Ifuku K (2013) Highly efficient
transformation of the diatom Phaeodactylum
tricornutum by multi-pulse electroporation.
Biosci Biotechnol Biochem 77:874–876
9. Ifuku K, Yan D, Miyahara M, Inoue-Kashino N,
Kashino Y (2015) A stable and efficient transformation system for the diatom Chaetoceros
gracilis. Photosynth Res 23:203–211
10. Tokushima H, Inoue-Kashino N, Nakazato Y,
Masuda A, Ifuku K, Kashino Y (2016) Advantageous characteristics of the diatom Chaetoceros gracilis as a sustainable biofuel producer.
Biotechnol Biofuels 9:235
11. Guillard RRL (1975) Culture of phytoplankton for feeding marine invertebrates. In: Smith
WL, Chanley MH (eds) Culture of marine
invertebrate animals. Springer, Boston, MA
12. Kajikawa M, Abe T, Ifuku K, Furutani KI,
Yan D, Okuda T, Ando A, Kishino S,
Ogawa J, Fukuzawa H (2016) Production of
ricinoleic acid-containing monoestolide triacylglycerides in an oleaginous diatom, Chaetoceros
gracilis. Sci Rep 6:36809
174
Kentaro Ifuku and Dongyi Yan
This work was supported by the Japan Science and Technology
Agency, Advanced Low Carbon Technology Research and Development Program to K.I. (JPMJAL1105).
References
1. Falkowski PG, Barber RT, Smetacek VV
(1998) Biogeochemical controls and feedbacks
on ocean primary production. Science
281:200–207
2. Prihoda J, Tanaka A, de Paula WB, Allen JF,
Tirichine L, Bowler C (2012) Chloroplastmitochondria cross-talk in diatoms. J Exp Bot
63:1543–1557
3. Levitan O, Dinamarca J, Hochman G, Falkowski PG (2014) Diatoms: a fossil fuel of the
future. Trends Biotechnol 32:117–124
4. Wang Y, Cai J, Jiang Y, Jiang X, Zhang D
(2013) Preparation of biosilica structures
from frustules of diatoms and their applications: current state and perspectives. Appl
Microbiol Biotechnol 97:453–460
5. Zaslavskaia LA, Lippmeier JC, Kroth PG,
Grossman AR, Apt KE (2000) Transformation
of the diatom Phaeodactylum tricornutum
(Bacillariophyceae) with a variety of selectable
marker and reporter genes. J Phycol
36:379–386
6. Poulsen N, Chesley PM, Kroger N (2006)
Molecular genetic manipulation of the diatom
Thalassiosira pseudonana (Bacillariophyceae). J
Phycol 42:1059–1065
7. Miyagawa-Yamaguchi A, Okami T, Kira N,
Yamaguchi H, Ohnishi K, Adachi M (2011)
Stable nuclear transformation of the diatom
Chaetoceros sp. Phycol Res 59:113–119
8. Miyahara M, Aoi M, Inoue-Kashino N,
Kashino Y, Ifuku K (2013) Highly efficient
transformation of the diatom Phaeodactylum
tricornutum by multi-pulse electroporation.
Biosci Biotechnol Biochem 77:874–876
9. Ifuku K, Yan D, Miyahara M, Inoue-Kashino N,
Kashino Y (2015) A stable and efficient transformation system for the diatom Chaetoceros
gracilis. Photosynth Res 23:203–211
10. Tokushima H, Inoue-Kashino N, Nakazato Y,
Masuda A, Ifuku K, Kashino Y (2016) Advantageous characteristics of the diatom Chaetoceros gracilis as a sustainable biofuel producer.
Biotechnol Biofuels 9:235
11. Guillard RRL (1975) Culture of phytoplankton for feeding marine invertebrates. In: Smith
WL, Chanley MH (eds) Culture of marine
invertebrate animals. Springer, Boston, MA
12. Kajikawa M, Abe T, Ifuku K, Furutani KI,
Yan D, Okuda T, Ando A, Kishino S,
Ogawa J, Fukuzawa H (2016) Production of
ricinoleic acid-containing monoestolide triacylglycerides in an oleaginous diatom, Chaetoceros
gracilis. Sci Rep 6:36809
174
Kentaro Ifuku and Dongyi Yan