11. Shimogawara K, Fujiwara S, Grossman A,
Usuda H (1998) High-efficiency transformation of Chlamydomonas reinhardtii by electroporation. Genetics 148:1821–1828
12. Kilian O, Benemann CSE, Niyogi KK, Vick B
(2011) High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp. Proc Natl Acad Sci U S A
108:21265–21269
13. Radakovits R, Jinkerson RE, Fuerstenberg SI,
Tae H, Settlage RE, Boore JL, Posewitz MC
(2012) Draft genome sequence and genetic
transformation of the oleaginous alga Nannochloropis gaditana. Nat Commun 3:686
14. Li F, Gao D, Hu H (2014) High-efficiency
nuclear transformation of the oleaginous
marine Nannochloropsis species using PCR
product.
Biosci
Biotechnol
Biochem
78:812–817
15. Niu Y-F, Yang Z-K, Zhang M-H, Zhu C-C,
Yang W-D, Liu J-S, Li H-Y (2012) Transformation of diatom Phaeodactylum tricornutum
by electroporation and establishment of inducible selection marker. BioTechniques 0:1–3
16. Miyahara M, Aoi M, Inoue-Kashino N,
Kashino Y, Ifuku K (2013) Highly efficient
transformation of the diatom Phaeodactylum
tricornutum by multi-pulse electroporation.
Biosci Biotechnol Biochem 77:874–876
17. Harrison PJ, Waters RE, Taylor FJR (1980) A
broad spectrum artificial seawater medium for
coastal and open ocean phytoplankton. J Phycol 16:28–35
18. Guillard RRL (1975) Culture of phytoplankton for feeding marine invertebrates. In: Smith
WL, Canley MH (eds) Culture of marine invertebrate animals. Plenum Press, New York,
pp 29–60
Phaeodactylum tricornutum
167
Usuda H (1998) High-efficiency transformation of Chlamydomonas reinhardtii by electroporation. Genetics 148:1821–1828
12. Kilian O, Benemann CSE, Niyogi KK, Vick B
(2011) High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp. Proc Natl Acad Sci U S A
108:21265–21269
13. Radakovits R, Jinkerson RE, Fuerstenberg SI,
Tae H, Settlage RE, Boore JL, Posewitz MC
(2012) Draft genome sequence and genetic
transformation of the oleaginous alga Nannochloropis gaditana. Nat Commun 3:686
14. Li F, Gao D, Hu H (2014) High-efficiency
nuclear transformation of the oleaginous
marine Nannochloropsis species using PCR
product.
Biosci
Biotechnol
Biochem
78:812–817
15. Niu Y-F, Yang Z-K, Zhang M-H, Zhu C-C,
Yang W-D, Liu J-S, Li H-Y (2012) Transformation of diatom Phaeodactylum tricornutum
by electroporation and establishment of inducible selection marker. BioTechniques 0:1–3
16. Miyahara M, Aoi M, Inoue-Kashino N,
Kashino Y, Ifuku K (2013) Highly efficient
transformation of the diatom Phaeodactylum
tricornutum by multi-pulse electroporation.
Biosci Biotechnol Biochem 77:874–876
17. Harrison PJ, Waters RE, Taylor FJR (1980) A
broad spectrum artificial seawater medium for
coastal and open ocean phytoplankton. J Phycol 16:28–35
18. Guillard RRL (1975) Culture of phytoplankton for feeding marine invertebrates. In: Smith
WL, Canley MH (eds) Culture of marine invertebrate animals. Plenum Press, New York,
pp 29–60
Phaeodactylum tricornutum
167