References
1. Merchant S, Prochnik S, Vallon O, Harris E,
Karpowicz S et al (2007) The Chlamydomonas
genome reveals the evolution of key animal and
plant functions. Science 318(5848):245–250
2. Boynton J, Gillham N, Harris E, Hosler J,
Johnson A, Jones A, Randolph-Anderson B,
Robertson D, Klein T, Shark K, Sanford J
(1998) Chloroplast transformation in Chlamydomonas with high velocity microprojectiles. Science 240(4858):1534–1538
3. Blowers A, Bogorad L, Shark K, Sanford J
(1989) Studies on Chlamydomonas chloroplast
transformation: foreign DNA can be stably
maintained in the chromosome. Plant Cell 1
(1):123–132
4. Kindle K (1990) High-frequency nuclear transformation of Chlamydomonas reinhardtii. Proc
Natl Acad Sci U S A 87(3):1228–1232
5. Shimogawara K, Fujiwara S, Grossman A, Usuda
H (1998) High-efficiency transformation of
Chlamydomonas reinhardtii by electroporation.
Genetics 148(4):1821–1828
6. Kinoshita T, Fukuzawa H, Shimada T, Saito T,
Matsuda Y (1992) Primary structure and expression of a gamete lytic enzyme in Chlamydomonas
reinhardtii: similarity of functional domains to
matrix metalloproteases. Proc Natl Acad Sci U S
A 89(10):4693–4697
7. Yamano T, Iguchi H, Fukuzawa H (2013) Rapid
transformation of Chlamydomonas reinhardtii
without cell-wall removal. J Biosci Bioeng 115
(6):691–694
8. Berthold P, Schmitt R, Mages W (2002) An
engineered Streptomyces hygroscopicus aph7
00
gene mediates dominant resistance against
hygromycin B in Chlamydomonas reinhardtii.
Protist 153(4):401–412
Transformation of Chlamydomonas reinhardtii
161
1. Merchant S, Prochnik S, Vallon O, Harris E,
Karpowicz S et al (2007) The Chlamydomonas
genome reveals the evolution of key animal and
plant functions. Science 318(5848):245–250
2. Boynton J, Gillham N, Harris E, Hosler J,
Johnson A, Jones A, Randolph-Anderson B,
Robertson D, Klein T, Shark K, Sanford J
(1998) Chloroplast transformation in Chlamydomonas with high velocity microprojectiles. Science 240(4858):1534–1538
3. Blowers A, Bogorad L, Shark K, Sanford J
(1989) Studies on Chlamydomonas chloroplast
transformation: foreign DNA can be stably
maintained in the chromosome. Plant Cell 1
(1):123–132
4. Kindle K (1990) High-frequency nuclear transformation of Chlamydomonas reinhardtii. Proc
Natl Acad Sci U S A 87(3):1228–1232
5. Shimogawara K, Fujiwara S, Grossman A, Usuda
H (1998) High-efficiency transformation of
Chlamydomonas reinhardtii by electroporation.
Genetics 148(4):1821–1828
6. Kinoshita T, Fukuzawa H, Shimada T, Saito T,
Matsuda Y (1992) Primary structure and expression of a gamete lytic enzyme in Chlamydomonas
reinhardtii: similarity of functional domains to
matrix metalloproteases. Proc Natl Acad Sci U S
A 89(10):4693–4697
7. Yamano T, Iguchi H, Fukuzawa H (2013) Rapid
transformation of Chlamydomonas reinhardtii
without cell-wall removal. J Biosci Bioeng 115
(6):691–694
8. Berthold P, Schmitt R, Mages W (2002) An
engineered Streptomyces hygroscopicus aph7
00
gene mediates dominant resistance against
hygromycin B in Chlamydomonas reinhardtii.
Protist 153(4):401–412
Transformation of Chlamydomonas reinhardtii
161
