References
1. Gaj T, Gersbach CA, Barbas CF 3rd (2013)
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering. Trends Biotechnol
31(7):397–405. https://doi.org/10.1016/j.
tibtech.2013.04.004
2. Cong L, Ran FA, Cox D, Lin S, Barretto R,
Habib N, Hsu PD, Wu X, Jiang W, Marraffini
LA, Zhang F (2013) Multiplex genome engineering using CRISPR/Cas systems. Science
339(6121):819–823.
https://doi.org/10.
1126/science.1231143
3. Hwang WY, Fu Y, Reyon D, Maeder ML, Tsai
SQ, Sander JD, Peterson RT, Yeh JR, Joung JK
(2013) Efficient genome editing in zebrafish
using a CRISPR-Cas system. Nat Biotechnol
31(3):227–229.
https://doi.org/10.1038/
nbt.2501
4. Wang H, Yang H, Shivalila CS, Dawlaty MM,
Cheng AW, Zhang F, Jaenisch R (2013)
One-step generation of mice carrying mutations
in multiple genes by CRISPR/Cas-mediated
genome engineering. Cell 153(4):910–918.
https://doi.org/10.1016/j.cell.2013.04.025
5. Hashimoto M, Takemoto T (2015) Electroporation enables the efficient mRNA delivery into
the mouse zygotes and facilitates CRISPR/
Cas9-based genome editing. Sci Rep 5:11315.
https://doi.org/10.1038/srep11315
6. Hashimoto M, Yamashita Y, Takemoto T
(2016) Electroporation of Cas9 protein/
sgRNA into early pronuclear zygotes generates
non-mosaic mutants in the mouse. Dev Biol 418
(1):1–9.
https://doi.org/10.1016/j.ydbio.
2016.07.017
7. Tanihara F, Takemoto T, Kitagawa E, Rao S, Do
LT, Onishi A, Yamashita Y, Kosugi C, Suzuki H,
Sembon S, Suzuki S, Nakai M, Hashimoto M,
Yasue A, Matsuhisa M, Noji S, Fujimura T,
Fuchimoto D, Otoi T (2016) Somatic cell
reprogramming-free generation of genetically
modified pigs. Sci Adv 2(9):e1600803. https://
doi.org/10.1126/sciadv.1600803
8. Behringer R (2014) Manipulating the mouse
embryo: a laboratory manual, 4th edn. Cold
Spring Harbor Laboratory Press, Cold Spring
Harbor, New York
126
Tatsuya Takemoto
1. Gaj T, Gersbach CA, Barbas CF 3rd (2013)
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering. Trends Biotechnol
31(7):397–405. https://doi.org/10.1016/j.
tibtech.2013.04.004
2. Cong L, Ran FA, Cox D, Lin S, Barretto R,
Habib N, Hsu PD, Wu X, Jiang W, Marraffini
LA, Zhang F (2013) Multiplex genome engineering using CRISPR/Cas systems. Science
339(6121):819–823.
https://doi.org/10.
1126/science.1231143
3. Hwang WY, Fu Y, Reyon D, Maeder ML, Tsai
SQ, Sander JD, Peterson RT, Yeh JR, Joung JK
(2013) Efficient genome editing in zebrafish
using a CRISPR-Cas system. Nat Biotechnol
31(3):227–229.
https://doi.org/10.1038/
nbt.2501
4. Wang H, Yang H, Shivalila CS, Dawlaty MM,
Cheng AW, Zhang F, Jaenisch R (2013)
One-step generation of mice carrying mutations
in multiple genes by CRISPR/Cas-mediated
genome engineering. Cell 153(4):910–918.
https://doi.org/10.1016/j.cell.2013.04.025
5. Hashimoto M, Takemoto T (2015) Electroporation enables the efficient mRNA delivery into
the mouse zygotes and facilitates CRISPR/
Cas9-based genome editing. Sci Rep 5:11315.
https://doi.org/10.1038/srep11315
6. Hashimoto M, Yamashita Y, Takemoto T
(2016) Electroporation of Cas9 protein/
sgRNA into early pronuclear zygotes generates
non-mosaic mutants in the mouse. Dev Biol 418
(1):1–9.
https://doi.org/10.1016/j.ydbio.
2016.07.017
7. Tanihara F, Takemoto T, Kitagawa E, Rao S, Do
LT, Onishi A, Yamashita Y, Kosugi C, Suzuki H,
Sembon S, Suzuki S, Nakai M, Hashimoto M,
Yasue A, Matsuhisa M, Noji S, Fujimura T,
Fuchimoto D, Otoi T (2016) Somatic cell
reprogramming-free generation of genetically
modified pigs. Sci Adv 2(9):e1600803. https://
doi.org/10.1126/sciadv.1600803
8. Behringer R (2014) Manipulating the mouse
embryo: a laboratory manual, 4th edn. Cold
Spring Harbor Laboratory Press, Cold Spring
Harbor, New York
126
Tatsuya Takemoto