Acknowledgments
R. Yang acknowledges the funding from the Nebraska Center for
Integrated Biomolecular Communication (NIH National Institutes of General Medical Sciences P20 GM113126), from
Nebraska Center for Nanomedicine (P30 GM127200), from EPSCoR First Award, and from NSF award 1826135.
References
1. Kim TK, Eberwine JH (2010) Mammalian cell
transfection: the present and the future. Anal
Bioanal Chem 397(8):3173–3178
2. Silva G et al (2011) Meganucleases and other
tools for targeted genome engineering: perspectives and challenges for gene therapy.
Curr Gene Ther 11(1):11–27
3. Fei Z, Wang S, Xie Y, Henslee BE, Koh CG,
Lee LJ (2007) Gene transfection of mammalian cells using membrane sandwich electroporation. Anal Chem 79(15):5719–5722
4. Neumann E, Schaefer-Ridder M, Wang Y,
Hofschneider P (1982) Gene transfer into
mouse lyoma cells by electroporation in high
electric fields. EMBO J 1(7):841–845
5. Kinosita K Jr, Tsong TY (1977) Formation and
resealing of pores of controlled sizes in human
erythrocyte membrane. Nature 268:438
6. Boukany PE et al (2011) Nanochannel electroporation delivers precise amounts of biomolecules into living cells. Nat Nanotechnol 6
(11):747
7. Canatella PJ, Karr JF, Petros JA, Prausnitz MR
(2001) Quantitative study of electroporationmediated molecular uptake and cell viability.
Biophys J 80(2):755–764
8. Cottle RN, Lee CM, Archer D, Bao G (2015)
Controlled delivery of β-globin-targeting
TALENs and CRISPR/Cas9 into mammalian
cells for genome editing using microinjection.
Sci Rep 5:16031
9. Kang W et al (2014) Microfluidic device for
stem cell differentiation and localized electroporation of postmitotic neurons. Lab Chip 14
(23):4486–4495
10. Kang W et al (2013) Nanofountain probe electroporation (NFP-E) of single cells. Nano Lett
13(6):2448–2457
11. Yang R, Lemaı ˆtre V, Huang C, Haddadi A,
McNaughton R, Espinosa HD (2018) Monoclonal cell line generation and CRISPR/Cas9
manipulation via single-cell electroporation.
Small 14(12):1702495
12. Chisti Y (2001) Hydrodynamic damage to animal cells. Crit Rev Biotechnol 21(2):67–110
13. Popovich ND, Wong S-S, Ufer S, Sakhrani V,
Paine D (2003) Electron-transfer kinetics at
ITO films influence of oxygen plasma. J Electrochem Soc 150(11):H255–H259
14. Tovar O, Tung L (1991) Electroporation of
cardiac cell membranes with monophasic or
biphasic rectangular pulses. Pacing Clin Electrophysiol 14(11 Pt 2):1887–1892
15. Wang M, Orwar O, Olofsson J, Weber SG
(2010) Single-cell electroporation. Anal Bioanal Chem 397(8):3235–3248
Microfluidic Device for Localized Electroporation
97
R. Yang acknowledges the funding from the Nebraska Center for
Integrated Biomolecular Communication (NIH National Institutes of General Medical Sciences P20 GM113126), from
Nebraska Center for Nanomedicine (P30 GM127200), from EPSCoR First Award, and from NSF award 1826135.
References
1. Kim TK, Eberwine JH (2010) Mammalian cell
transfection: the present and the future. Anal
Bioanal Chem 397(8):3173–3178
2. Silva G et al (2011) Meganucleases and other
tools for targeted genome engineering: perspectives and challenges for gene therapy.
Curr Gene Ther 11(1):11–27
3. Fei Z, Wang S, Xie Y, Henslee BE, Koh CG,
Lee LJ (2007) Gene transfection of mammalian cells using membrane sandwich electroporation. Anal Chem 79(15):5719–5722
4. Neumann E, Schaefer-Ridder M, Wang Y,
Hofschneider P (1982) Gene transfer into
mouse lyoma cells by electroporation in high
electric fields. EMBO J 1(7):841–845
5. Kinosita K Jr, Tsong TY (1977) Formation and
resealing of pores of controlled sizes in human
erythrocyte membrane. Nature 268:438
6. Boukany PE et al (2011) Nanochannel electroporation delivers precise amounts of biomolecules into living cells. Nat Nanotechnol 6
(11):747
7. Canatella PJ, Karr JF, Petros JA, Prausnitz MR
(2001) Quantitative study of electroporationmediated molecular uptake and cell viability.
Biophys J 80(2):755–764
8. Cottle RN, Lee CM, Archer D, Bao G (2015)
Controlled delivery of β-globin-targeting
TALENs and CRISPR/Cas9 into mammalian
cells for genome editing using microinjection.
Sci Rep 5:16031
9. Kang W et al (2014) Microfluidic device for
stem cell differentiation and localized electroporation of postmitotic neurons. Lab Chip 14
(23):4486–4495
10. Kang W et al (2013) Nanofountain probe electroporation (NFP-E) of single cells. Nano Lett
13(6):2448–2457
11. Yang R, Lemaı ˆtre V, Huang C, Haddadi A,
McNaughton R, Espinosa HD (2018) Monoclonal cell line generation and CRISPR/Cas9
manipulation via single-cell electroporation.
Small 14(12):1702495
12. Chisti Y (2001) Hydrodynamic damage to animal cells. Crit Rev Biotechnol 21(2):67–110
13. Popovich ND, Wong S-S, Ufer S, Sakhrani V,
Paine D (2003) Electron-transfer kinetics at
ITO films influence of oxygen plasma. J Electrochem Soc 150(11):H255–H259
14. Tovar O, Tung L (1991) Electroporation of
cardiac cell membranes with monophasic or
biphasic rectangular pulses. Pacing Clin Electrophysiol 14(11 Pt 2):1887–1892
15. Wang M, Orwar O, Olofsson J, Weber SG
(2010) Single-cell electroporation. Anal Bioanal Chem 397(8):3235–3248
Microfluidic Device for Localized Electroporation
97