223
Fig. 6.14 Deep eutectic solvent-based vortex extraction combined with emulsification liquidliquid microextraction. Deep eutectic solvent (DES) was injected rapidly to the sample solution
with tetrahydrofuran as an emulsifier agent. This leads to the formation of a cloudy solution due to
insoluble self-aggregation of DES droplets. The cloudy solution was subjected to ultrasonication.
Then centrifugation was applied to separate the DES phase from the aqueous solution. The DES
phase containing curcumin was subjected to UV-Vis analysis. (Figure reprinted with permission
from Aydin et al. 2018)
Fig. 6.15 Dispersive solid-phase extraction in combination with deep eutectic solvent (DES)based air-assisted liquid-liquid microextraction preconcentration procedure. The process started
with diluted urine or plasma samples transferred into a glass test tube in the presence of sorbent.
The tube was then vortexed. Then, the adsorbed analytes were subsequently eluted with DES under
sonication. The supernatant solution obtained from the previous step was removed and then subjected to five aspiration/dispersion cycles. A cloudy solution was formed. After centrifugation,
DES phase was analyzed. (Figure reprinted with permission from Mohebbi et al. 2018)
6 Methods for Extraction of Bioactive Compounds from Plant and Animal Matter…
Fig. 6.14 Deep eutectic solvent-based vortex extraction combined with emulsification liquidliquid microextraction. Deep eutectic solvent (DES) was injected rapidly to the sample solution
with tetrahydrofuran as an emulsifier agent. This leads to the formation of a cloudy solution due to
insoluble self-aggregation of DES droplets. The cloudy solution was subjected to ultrasonication.
Then centrifugation was applied to separate the DES phase from the aqueous solution. The DES
phase containing curcumin was subjected to UV-Vis analysis. (Figure reprinted with permission
from Aydin et al. 2018)
Fig. 6.15 Dispersive solid-phase extraction in combination with deep eutectic solvent (DES)based air-assisted liquid-liquid microextraction preconcentration procedure. The process started
with diluted urine or plasma samples transferred into a glass test tube in the presence of sorbent.
The tube was then vortexed. Then, the adsorbed analytes were subsequently eluted with DES under
sonication. The supernatant solution obtained from the previous step was removed and then subjected to five aspiration/dispersion cycles. A cloudy solution was formed. After centrifugation,
DES phase was analyzed. (Figure reprinted with permission from Mohebbi et al. 2018)
6 Methods for Extraction of Bioactive Compounds from Plant and Animal Matter…