193
was processed this year, barely enough for producing about a 100 tons betacyclodextrin quantity.
The Chinoin’s cyclodextrin glucosyltransferase product was a highly purified
preparation isolated from B. macerans fermentation liquor and concentrated by
about 200-fold related to its original specific activity by affinity chromatography
(Seres and László 1982). The enzyme was available as a white odorless freeze-dried
powder shipped in 5 mL vials under reduced pressure.
In 1984 and 1985, 500 and 600 vials were sold as fine chemical via LucernaChem AG. At that time only Chinoin and Amano Pharmaceutical Co. (Japan) produced this enzyme in purified form. Specification of the purified enzyme
manufactured by Chinoin Pharmaceutical and Chemical Works Co. Ltd. was set up
(Table 4.2).
At this time, the management of Chinoin was considering purified enzyme production for sale. As part of new economical era, a special new firm formation, socalled part-time work cooperative, the Cyclodextrin VGM was established in the
factory in the late 1980s. This VGM was responsible for the affinity chromatographic manufacturing and qualification of the product, with a cooperation with
Central Analytical Department of Chinoin. Due to lack of any export license, both
sales and distribution of product were managed by an international cooperation
between Reanal Fine Chemicals Co. (Budapest, Hungary) and LucernaChem AG
(Luzern, Switzerland) (Fig. 4.7).
In the late 1980s, additional attempts were made to purify further this lyophilized
cyclodextrin glucosyltransferase enzyme product by gel filtration in pilot plant
scale. After separation by gel chromatography on Sephacryl-S-200, the resulting
fractions were lyophilized on a Leybold-Heraeus industrial equipment (Seres and
Fig. 4.7 Photograph of the
exported pure
glucosyltransferase
enzyme product
4 History of Cyclodextrin Production in Hungary
was processed this year, barely enough for producing about a 100 tons betacyclodextrin quantity.
The Chinoin’s cyclodextrin glucosyltransferase product was a highly purified
preparation isolated from B. macerans fermentation liquor and concentrated by
about 200-fold related to its original specific activity by affinity chromatography
(Seres and László 1982). The enzyme was available as a white odorless freeze-dried
powder shipped in 5 mL vials under reduced pressure.
In 1984 and 1985, 500 and 600 vials were sold as fine chemical via LucernaChem AG. At that time only Chinoin and Amano Pharmaceutical Co. (Japan) produced this enzyme in purified form. Specification of the purified enzyme
manufactured by Chinoin Pharmaceutical and Chemical Works Co. Ltd. was set up
(Table 4.2).
At this time, the management of Chinoin was considering purified enzyme production for sale. As part of new economical era, a special new firm formation, socalled part-time work cooperative, the Cyclodextrin VGM was established in the
factory in the late 1980s. This VGM was responsible for the affinity chromatographic manufacturing and qualification of the product, with a cooperation with
Central Analytical Department of Chinoin. Due to lack of any export license, both
sales and distribution of product were managed by an international cooperation
between Reanal Fine Chemicals Co. (Budapest, Hungary) and LucernaChem AG
(Luzern, Switzerland) (Fig. 4.7).
In the late 1980s, additional attempts were made to purify further this lyophilized
cyclodextrin glucosyltransferase enzyme product by gel filtration in pilot plant
scale. After separation by gel chromatography on Sephacryl-S-200, the resulting
fractions were lyophilized on a Leybold-Heraeus industrial equipment (Seres and
Fig. 4.7 Photograph of the
exported pure
glucosyltransferase
enzyme product
4 History of Cyclodextrin Production in Hungary