3 Block Copolypeptide Synthesis and Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
3.1 Copolypeptide Nanoparticles with Hydrophobic Cores . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
3.2 Copolypeptide Vesicles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
3.3 Copolypeptide Hydrogels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
4 Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Abbreviations
AM
Activated monomer
ATRP
Atom transfer radical polymerization
Bn-Asp
β-Benzyl-L-aspartate
Bn-Glu
γ-Benzyl-L-glutamate
Bn-Tyr
O-Benzyl-L-tyrosine
bpy
2,2
0 -Bipyridine
CNS
Central nervous system
depe
Bis(diethylphosphino)ethane
DIC
Differential interference contrast
DLS
Dynamic light scattering
DMEM
Dulbecco’s modified Eagle’s medium
DOPA
L-Dihydroxyphenylalanine
GPC
Gel permeation chromatography
HMDS
Hexamethyldisilazane
LSCM
Laser scanning confocal microscopy
MALDI-MS
Matrix-assisted laser desorption ionization–mass
spectroscopy
NACE
Non-aqueous capillary electrophoresis
NCA
α-Amino acid N-carboxyanhydride
NGF
Nerve growth factor
PA
Poly(L-alanine)
PBLA
Poly(β-benzyl-L-aspartate)
PBLG
Poly(γ-benzyl-L-glutamate)
PBS
Phosphate buffered saline
PDMS
Polydimethylsiloxane
PEG
Polyethylene glycol
PMDG
Poly(γ-methyl-D-glutamate)
PMLG
Poly(γ-methyl-L-glutamate)
PPG
Poly(racemic-propargylglycine)
PZLL
Poly(ε-carbobenzyloxy-L-lysine)
ROMP
Ring-opening metathesis polymerization
TEM
Transmission electron microscopy
TFA-Lys
ε-Trifluoroacetyl-L-lysine
TMS
Trimethylsilyl
2
T.J. Deming