PCC-a-M, 83
chemical structure, 83
in the X-ray structure of cytochrome b 6 f, 78, 79
PDS, see Potassium dodecylsulfate
PE, see Phosphatidylethanolamine (PE)
PEG, see Polyethylene glycol
Pentameric ligand-gated ion channels (pLGICs), 39
conformational transitions, 39
during activation and desensitization, 43
underlying gating, 40
coupling between the extracellular and TM regions,
39, 41
quaternary structure, 38
Peptergents, 121–123
Peptide-based NDs, 127
MD simulation, 127
Peptidoglycan, 4
Peptitergents, 118, 119, 134
overview, 120
PepT So2 peptide transporter
cryoEM structure in complex with saposin A, 126
perDAPol (perdeuterated A8-35), see A8-35 derivatives,
perDAPol
Peripherin/ROM1 complex
SP NS EM study in A8-35, 557
Periplasm, 4
Persistence length, 153
Persistent radical effect (PRE) system, 220
PG, see Phosphatidylglycerol (PG)
P-glycoprotein 1 (PgP)
lipid binding in SMALPs, 278
SP cryo-EM reconstruction in SMA, 569
PgP, see P-glycoprotein 1 (PgP)
Phage display selection of MP binders
using biotinylated APols, 604–606
PhoE
insolubility in peptitergents, 120
Phosphatidic acid (PA)
chemical structure, 3
role in nAChR coupling, 43
Phosphatidylcholine (PC)
chemical structure, 3
specific binding to cytochrome c oxidase, 27
structures formed in water, 5
Phosphatidylethanolamine (PE)
chemical structure, 3
specific binding to bacterial RC, 27
structures formed in water, 5
Phosphatidylglycerol (PG)
chemical structure, 3
as a cofactor of PSI, 29, 30
specific binding to KcsA, 27, 29
Phosphatidylinositol (PI)
chemical structure, 3
Phosphatidylserine (PS), 6
chemical structure, 3
Phospholamban
engineering a water-soluble version, 88
Phosphorylcholine-based APols (PC-APols), 249, 252
assembly, 193
biotinylated derivative, 198
chemical structure, 158, 162–163
synthesis, 162–163
Photosynthesis, 6
Photosynthetic antenna complexes, 32
Photosynthetic complexes
extraction by APol/detergent mixtures, 261
Photosynthetic reaction centers (RCs)
APol binding, 274
bound PE, 27
composition of RC/A8-75 complexes, 273
first X-ray structure, 13
lipid composition in SMALPs, 277, 278
SGSV analysis of RC/A8-75 complexes, 413
stability in SMALPs, 294, 295
TM structure, 16
Photosystem I (PSI) reaction center
protein-buried lipids, 29, 30
stabilization by designer peptide surfactants,
122, 123
TM structure, 17
PI, see Phosphatidylinositol (PI)
Picard, M., 524
Picodiscs, 119, 134
proposed structure, 124
¼ Sap A discs, Salipro
® nanoparticles, 123–127
Plasma membrane, 4, 6
pLGICs, see Pentameric ligand-gated ion channels
(pLGICs)
Plückthun, A., 604
PMAL, 252
PMAL series
chemical structure, 163
PMALA-C12, 299
assembly, 194
SEC analysis, 194
PMAL-B series
chemical structure, 158
PMAL-B-0
chemical structure, 163
PMAL-B-50
chemical structure, 163
PMAL-B-100, 289
chemical structure, 163
PMAL-C8, 264, 572
chemical structure, 163
PMAL-C12, 290, 299
assembly, 194
chemical structure, 163
SEC analysis, 194
sensitivity to divalent cations, 419, 420
sensitivity to multivalent cations, 194
PMAL-C16
chemical structure, 163
Poly[N-(2-hydroxypropyl)-methacryl-amide-cododecylmethacrylate], 152
Index
703
chemical structure, 83
in the X-ray structure of cytochrome b 6 f, 78, 79
PDS, see Potassium dodecylsulfate
PE, see Phosphatidylethanolamine (PE)
PEG, see Polyethylene glycol
Pentameric ligand-gated ion channels (pLGICs), 39
conformational transitions, 39
during activation and desensitization, 43
underlying gating, 40
coupling between the extracellular and TM regions,
39, 41
quaternary structure, 38
Peptergents, 121–123
Peptide-based NDs, 127
MD simulation, 127
Peptidoglycan, 4
Peptitergents, 118, 119, 134
overview, 120
PepT So2 peptide transporter
cryoEM structure in complex with saposin A, 126
perDAPol (perdeuterated A8-35), see A8-35 derivatives,
perDAPol
Peripherin/ROM1 complex
SP NS EM study in A8-35, 557
Periplasm, 4
Persistence length, 153
Persistent radical effect (PRE) system, 220
PG, see Phosphatidylglycerol (PG)
P-glycoprotein 1 (PgP)
lipid binding in SMALPs, 278
SP cryo-EM reconstruction in SMA, 569
PgP, see P-glycoprotein 1 (PgP)
Phage display selection of MP binders
using biotinylated APols, 604–606
PhoE
insolubility in peptitergents, 120
Phosphatidic acid (PA)
chemical structure, 3
role in nAChR coupling, 43
Phosphatidylcholine (PC)
chemical structure, 3
specific binding to cytochrome c oxidase, 27
structures formed in water, 5
Phosphatidylethanolamine (PE)
chemical structure, 3
specific binding to bacterial RC, 27
structures formed in water, 5
Phosphatidylglycerol (PG)
chemical structure, 3
as a cofactor of PSI, 29, 30
specific binding to KcsA, 27, 29
Phosphatidylinositol (PI)
chemical structure, 3
Phosphatidylserine (PS), 6
chemical structure, 3
Phospholamban
engineering a water-soluble version, 88
Phosphorylcholine-based APols (PC-APols), 249, 252
assembly, 193
biotinylated derivative, 198
chemical structure, 158, 162–163
synthesis, 162–163
Photosynthesis, 6
Photosynthetic antenna complexes, 32
Photosynthetic complexes
extraction by APol/detergent mixtures, 261
Photosynthetic reaction centers (RCs)
APol binding, 274
bound PE, 27
composition of RC/A8-75 complexes, 273
first X-ray structure, 13
lipid composition in SMALPs, 277, 278
SGSV analysis of RC/A8-75 complexes, 413
stability in SMALPs, 294, 295
TM structure, 16
Photosystem I (PSI) reaction center
protein-buried lipids, 29, 30
stabilization by designer peptide surfactants,
122, 123
TM structure, 17
PI, see Phosphatidylinositol (PI)
Picard, M., 524
Picodiscs, 119, 134
proposed structure, 124
¼ Sap A discs, Salipro
® nanoparticles, 123–127
Plasma membrane, 4, 6
pLGICs, see Pentameric ligand-gated ion channels
(pLGICs)
Plückthun, A., 604
PMAL, 252
PMAL series
chemical structure, 163
PMALA-C12, 299
assembly, 194
SEC analysis, 194
PMAL-B series
chemical structure, 158
PMAL-B-0
chemical structure, 163
PMAL-B-50
chemical structure, 163
PMAL-B-100, 289
chemical structure, 163
PMAL-C8, 264, 572
chemical structure, 163
PMAL-C12, 290, 299
assembly, 194
chemical structure, 163
SEC analysis, 194
sensitivity to divalent cations, 419, 420
sensitivity to multivalent cations, 194
PMAL-C16
chemical structure, 163
Poly[N-(2-hydroxypropyl)-methacryl-amide-cododecylmethacrylate], 152
Index
703