NAPols, see Glucose-based non-ionic APols; Non-ionic
APols (NAPols)
NBD-labeled A8-35, see A8-35 derivatives, FAPol NBD
N-dodecyl-β-D-maltopyranoside, see Dodecylmaltoside
(DDM)
NDs, see Nanodiscs (NDs)
Negative staining (NS), 535
Neuromuscular junction, 8, 36
Neuropilin-1 (NRP1)
delivery of A8-35-trapped TM α-helix to cells in
culture, 672
distribution of TM α-helix in cells following A8-35mediated delivery, 673
physiological role, 672
TM α-helix, 672
use of TM α-helix in cancer therapy, 672
Neurospora crassa
complex I, 550
Neurotensin receptor (NTS1)
conformational thermostabilization, 87
Neutron diffraction
by MP crystals, 69
Neutze, R., 35
Nicolson, G.L., 22
Nicotinic acetylcholine receptor (nAChR), 39
A8-35 binding, 274
ACh binding sites, 38
activation and desensitization, 37
allosteric conformational transitions, 37
allosteric equilibria, 287
allosteric transitions in membrane, detergent and APol
environments, 288
coupling between the extracellular and TM regions, 43
EM view of conformational transitions, 42
function, 10
immobilization using BAPol, 595
main conformational states, 37
overall structure, 39
rebinding of lipids upon transfer to A8-35, 277
recognition by α-Bgt following immobilization using
BAPol, 598
reconstitution into lipid vesicles, 76
restoration of allosteric equilibria following trapping
with A8-35, 287
role of lipids in activation and desensitization, 43
structure and function (overview), 36–43
TM topology, 14
transmembrane topology and quaternary structure, 37
X-ray structure, 41, 42
Nietlispach, D., 458
Nitroxide-mediated polymerization (NMP), 219
NMP, see Nitroxide-mediated polymerization (NMP)
N-octyl-β-D-glucopyranoside, see Octylglucoside (OG)
Non-conventional surfactants
amphipols, 151–152
compared properties, 133–135
overview, 98, 133–135
Non-ionic APols (NAPols), 249, 252
biotinylated (BNAPols), 604
comparison of NMR spectra of OmpX in NAPol
vs. A8-35, 478
NMR studies of OmpX/NAPol complexes, 477, 478
use for CFE of BR, 367, 372, 373
use for folding MPs, 345
use for sNMR, 477, 478
NRP1, see Neuropilin-1 (NRP1)
NS, see Negative staining
Nuclear magnetic resonance (NMR), 453
Nuclear membrane, 6
NVoy (NV10), 166, 169, 249, 252, 282, 289
chemical structure, 166
particles, 197
use for CFE, 369
use for CFE of GPCRs, 367, 370
use for NMR studies, 479
O
OAPA-20
chemical structure, 158, 160
Octylglucoside (OG), 62, 81
chemical structure, 61
CMC, 61
residency time in micelles, 65
Octylthioglucoside (OTG)
chemical structure, 61
CMC, 61
OG, see Octylglucoside (OG)
OligAPol, 198
SEC analysis, 204
SEC analysis of BR/OligAPol complexes, 448
(see also A8-35 derivatives, OligAPol)
Oligonucleotide-tagged A8-35 (OligAPol)
SEC analysis of BR/OligAPol complexes, 448
(see also A8-35 derivatives, OligAPol)
OmpA (E. coli) transmembrane domain (tOmpA)
A8-35 binding, 283
APol-assisted folding, 340, 348
behavior of tOmpA/A8-35 complexes upon separation
from free APol, 257
calorimetric titration of transfer from detergent to
PC-APol, 254
comparative ESI-MS analyses of various tOmpA/APol
complexes, 639
comparison of 2D NMR spectra of tOmpA/SAPol
complexes at 30, 50 and 70
C, 476
determination of rotational correlation time of tOmpA/
A8-35 complexes by solution NMR, 422–423
displacement of bound A8-35 by detergent, 298, 308
FRET with FAPol NBD , 298
immobilization using BAPol, 595, 604
immobilization using OligAPol, 614
intermolecular tOmpA/A8-35 contacts mapped by
NMR, 470
MALDI-TOF studies of tOmpA/APol complexes,
633, 634
NAPol-trapped, 167
NMR studies of tOmpA/SAPol complexes,
474, 475
Index
701
APols (NAPols)
NBD-labeled A8-35, see A8-35 derivatives, FAPol NBD
N-dodecyl-β-D-maltopyranoside, see Dodecylmaltoside
(DDM)
NDs, see Nanodiscs (NDs)
Negative staining (NS), 535
Neuromuscular junction, 8, 36
Neuropilin-1 (NRP1)
delivery of A8-35-trapped TM α-helix to cells in
culture, 672
distribution of TM α-helix in cells following A8-35mediated delivery, 673
physiological role, 672
TM α-helix, 672
use of TM α-helix in cancer therapy, 672
Neurospora crassa
complex I, 550
Neurotensin receptor (NTS1)
conformational thermostabilization, 87
Neutron diffraction
by MP crystals, 69
Neutze, R., 35
Nicolson, G.L., 22
Nicotinic acetylcholine receptor (nAChR), 39
A8-35 binding, 274
ACh binding sites, 38
activation and desensitization, 37
allosteric conformational transitions, 37
allosteric equilibria, 287
allosteric transitions in membrane, detergent and APol
environments, 288
coupling between the extracellular and TM regions, 43
EM view of conformational transitions, 42
function, 10
immobilization using BAPol, 595
main conformational states, 37
overall structure, 39
rebinding of lipids upon transfer to A8-35, 277
recognition by α-Bgt following immobilization using
BAPol, 598
reconstitution into lipid vesicles, 76
restoration of allosteric equilibria following trapping
with A8-35, 287
role of lipids in activation and desensitization, 43
structure and function (overview), 36–43
TM topology, 14
transmembrane topology and quaternary structure, 37
X-ray structure, 41, 42
Nietlispach, D., 458
Nitroxide-mediated polymerization (NMP), 219
NMP, see Nitroxide-mediated polymerization (NMP)
N-octyl-β-D-glucopyranoside, see Octylglucoside (OG)
Non-conventional surfactants
amphipols, 151–152
compared properties, 133–135
overview, 98, 133–135
Non-ionic APols (NAPols), 249, 252
biotinylated (BNAPols), 604
comparison of NMR spectra of OmpX in NAPol
vs. A8-35, 478
NMR studies of OmpX/NAPol complexes, 477, 478
use for CFE of BR, 367, 372, 373
use for folding MPs, 345
use for sNMR, 477, 478
NRP1, see Neuropilin-1 (NRP1)
NS, see Negative staining
Nuclear magnetic resonance (NMR), 453
Nuclear membrane, 6
NVoy (NV10), 166, 169, 249, 252, 282, 289
chemical structure, 166
particles, 197
use for CFE, 369
use for CFE of GPCRs, 367, 370
use for NMR studies, 479
O
OAPA-20
chemical structure, 158, 160
Octylglucoside (OG), 62, 81
chemical structure, 61
CMC, 61
residency time in micelles, 65
Octylthioglucoside (OTG)
chemical structure, 61
CMC, 61
OG, see Octylglucoside (OG)
OligAPol, 198
SEC analysis, 204
SEC analysis of BR/OligAPol complexes, 448
(see also A8-35 derivatives, OligAPol)
Oligonucleotide-tagged A8-35 (OligAPol)
SEC analysis of BR/OligAPol complexes, 448
(see also A8-35 derivatives, OligAPol)
OmpA (E. coli) transmembrane domain (tOmpA)
A8-35 binding, 283
APol-assisted folding, 340, 348
behavior of tOmpA/A8-35 complexes upon separation
from free APol, 257
calorimetric titration of transfer from detergent to
PC-APol, 254
comparative ESI-MS analyses of various tOmpA/APol
complexes, 639
comparison of 2D NMR spectra of tOmpA/SAPol
complexes at 30, 50 and 70
C, 476
determination of rotational correlation time of tOmpA/
A8-35 complexes by solution NMR, 422–423
displacement of bound A8-35 by detergent, 298, 308
FRET with FAPol NBD , 298
immobilization using BAPol, 595, 604
immobilization using OligAPol, 614
intermolecular tOmpA/A8-35 contacts mapped by
NMR, 470
MALDI-TOF studies of tOmpA/APol complexes,
633, 634
NAPol-trapped, 167
NMR studies of tOmpA/SAPol complexes,
474, 475
Index
701