Looking ahead to medical applications
(# 2018 by Francis Haraux).
The results available to date fall into two categories: (i) demonstrating that APols can be
advantageously used to formulate subunit vaccines that present a better efficacy, thermal resistance,
and shelf life than their detergent-based analogs, and (ii) examining the fate of APols and their cargoes
when applied to cells in culture and their distribution and elimination when injected in mice. In addition,
APols carrying various adjuvants have been synthesized. Relevant studies are listed in Table 15.1.
Table 15.1 Some studies relevant to biomedical uses of amphipols.
1
Study Field
Comments
References
15.1 Cell biology
Visualization of the internalization of A8-35 and a
passenger transmembrane peptide by cells in culture
and of the access of a fluorescent APol to tumors in
live mice
Popot et al. (2011)
15.2 Vaccinology
Compared protections afforded by detergent- and
A8-35-based vaccines against Chlamydia
trachomatis
Tifrea et al. (2011)
15.3 Biochemistry/
biophysics
Stability, solution properties, and shelf life of the
major outer membrane protein from C. trachomatis
in detergent solution vs. an A8-35-trapped form
Feinstein et al. (2014)
15.4 Vaccinology
Investigations into the origins of the better protection
afforded by an A8-35- vs. a detergent-based vaccine
against C. muridarum
Tifrea et al. (2014)
15.5 Formulation
Distribution and elimination of A8-35 following
injection in mice by various routes
Fernandez et al.
(2014)
15.6 Chemistry/
physical
chemistry
Synthesis and characterization of several adjuvantcarrying versions of A8-35
Le Bon et al. (2014a,
b), and Tifrea et al.
(2018a, b)
15.7 Vaccinology
Synthesis of an APol carrying a covalently bound
peptidic adjuvant and demonstration that using the
resulting “vaccipol” to trap and deliver a MP used as
an immunogen can improve the immune response as
compared to the use of the free adjuvant
Tifrea et al. (2018b)
1
Published too late to be discussed here is a study in which a membrane-associated amyloid-β oligomer
potentially relevant in Alzheimer’s disease was stabilized by non-ionic APols (Serra-Batiste et al. 2018)
660
15 Biomedical Applications
(# 2018 by Francis Haraux).
The results available to date fall into two categories: (i) demonstrating that APols can be
advantageously used to formulate subunit vaccines that present a better efficacy, thermal resistance,
and shelf life than their detergent-based analogs, and (ii) examining the fate of APols and their cargoes
when applied to cells in culture and their distribution and elimination when injected in mice. In addition,
APols carrying various adjuvants have been synthesized. Relevant studies are listed in Table 15.1.
Table 15.1 Some studies relevant to biomedical uses of amphipols.
1
Study Field
Comments
References
15.1 Cell biology
Visualization of the internalization of A8-35 and a
passenger transmembrane peptide by cells in culture
and of the access of a fluorescent APol to tumors in
live mice
Popot et al. (2011)
15.2 Vaccinology
Compared protections afforded by detergent- and
A8-35-based vaccines against Chlamydia
trachomatis
Tifrea et al. (2011)
15.3 Biochemistry/
biophysics
Stability, solution properties, and shelf life of the
major outer membrane protein from C. trachomatis
in detergent solution vs. an A8-35-trapped form
Feinstein et al. (2014)
15.4 Vaccinology
Investigations into the origins of the better protection
afforded by an A8-35- vs. a detergent-based vaccine
against C. muridarum
Tifrea et al. (2014)
15.5 Formulation
Distribution and elimination of A8-35 following
injection in mice by various routes
Fernandez et al.
(2014)
15.6 Chemistry/
physical
chemistry
Synthesis and characterization of several adjuvantcarrying versions of A8-35
Le Bon et al. (2014a,
b), and Tifrea et al.
(2018a, b)
15.7 Vaccinology
Synthesis of an APol carrying a covalently bound
peptidic adjuvant and demonstration that using the
resulting “vaccipol” to trap and deliver a MP used as
an immunogen can improve the immune response as
compared to the use of the free adjuvant
Tifrea et al. (2018b)
1
Published too late to be discussed here is a study in which a membrane-associated amyloid-β oligomer
potentially relevant in Alzheimer’s disease was stabilized by non-ionic APols (Serra-Batiste et al. 2018)
660
15 Biomedical Applications