Christman, K.L., Requa, M.V., Enriquez-Rios, V.D., Ward, S.C., Bradley, K.A., Turner, K.L., Maynard, H.D. (2006b)
Submicron streptavidin patterns for protein assembly. Langmuir 22:7444–7450.
Cosnier, S., Galland, B., Gondran, C., Le Pellec, A. (1998) Electrogeneration of biotinylated functionalized polypyrroles
for the simple immobilization of enzymes. Electroanalysis 10:808–813.
Cosnier, S., Stoytcheva, M., Senillou, A., Perrot, H., Furriel, R.P.M., Leone, F.A. (1999) A biotinylated conducting
polypyrrole for the spatially controlled construction of an amperometric biosensor. Anal. Chem. 71:3692–3697.
Coyer, S.R., García, A.J., Delamarche, E. (2007) Facile preparation of complex protein architectures with sub-100-nm
resolution on surfaces. Angew. Chem. Int. Ed. 46:6837–6840.
Dahmane, T., Damian, M., Mary, S., Popot, J.-L., Banères, J.-L. (2009) Amphipol-assisted in vitro folding of G proteincoupled receptors. Biochemistry 48:6516–6521.
Darmanin, T., Bellanger, H., Guittard, F., Lisboa, P., Zurn, M., Colpo, P., Gilliland, D., Rossi, F. (2012) Structured
biotinylated poly(3,4-ethylenedioxypyrrole) electrodes for biochemical applications. RSC Adv. 2:1033–1039.
Della Pia, E.A., Holm, J., Lloret, N., Le Bon, C., Popot, J.-L., Zoonens, M., Nygård, J., Martinez, K.L. (2014a) A step
closer to membrane protein multiplexed nano-arrays using biotin-doped polypyrrole. ACS Nano 8:1844–1853.
Della Pia, E.A., Westh Hansen, R., Zoonens, M., Martinez, K.L. (2014b) Functionalized amphipols: A versatile toolbox
suitable for applications of membrane proteins in synthetic biology. J. Membr. Biol. 247:815–826.
Fang, Y., Frutos, A.G., Lahiri, J. (2002) Membrane protein microarrays. J. Am. Chem. Soc. 124:2394–2395.
Fernandez, A., Le Bon, C., Baumlin, N., Giusti, F., Crémel, G., Popot, J.-L., Bagnard, D. (2014) In vivo characterization
of the biodistribution profile of amphipols J. Membr. Biol. 247:1043–1051.
Ferrandez, Y., Dezi, M., Bosco, M., Urvoas, A., Valério, M., Le Bon, C., Giusti, F., Broutin, I., Durand, G., Polidori, A.,
Popot, J.-L., Picard, M., Minard, P. (2014) Amphipol-mediated screening of molecular orthoses specific for
membrane protein targets. J. Membr. Biol. 247:925–940.
Franklin, G.I., Potter, L.T. (1972) Studies of the binding of α-bungarotoxin to membrane-bound and detergent-dispersed
acetylcholine receptors from Torpedo electric tissue. FEBS Lett. 28:101–106.
Friedrich, M.G., Giess, F., Naumann, R., Knoll, W., Ataka, K., Heberle, J., Hrabakova, J., Murgida, D.H., Hildebrandt,
P. (2004) Active site structure and redox processes of cytochrome c oxidase immobilised in a novel biomimetic lipid
membrane on an electrode. Chem. Commun. 21:2376–2377.
Früh, V., IJzerman, A.P., Siegal, G. (2011) How to catch a membrane protein in action: a review of functional membrane
protein immobilization strategies and their applications. Chem. Rev. 111:640–656.
Giusti, F., Kessler, P., Westh Hansen, R., Della Pia, E.A., Le Bon, C., Mourier, G., Popot, J.-L., Martinez, K.L., Zoonens,
M. (2015) Synthesis of a polyhistidine-bearing amphipol and its use for immobilizing membrane proteins. Biomacromolecules 16:3751–3761.
Giusti, F., Rieger, J., Catoire, L., Qian, S., Calabrese, A.N., Watkinson, T.G., Casiraghi, M., Radford, S.E., Ashcroft, A.
E., Popot, J.-L. (2014) Synthesis, characterization and applications of a perdeuterated amphipol. J. Membr. Biol.
247:909–924.
Gohon, Y., Giusti, F., Prata, C., Charvolin, D., Timmins, P., Ebel, C., Tribet, C., Popot, J.-L. (2006) Well-defined
nanoparticles formed by hydrophobic assembly of a short and polydisperse random terpolymer, amphipol A8-35.
Langmuir 22:1281–1290.
Gohon, Y., Pavlov, G., Timmins, P., Tribet, C., Popot, J.-L., Ebel, C. (2004) Partial specific volume and solvent
interactions of amphipol A8-35. Anal. Biochem. 334:318–334.
Gottschalk, I., Li, Y.M., Lundahl, P. (2000) Chromatography on cells: analyses of solute interactions with the glucose
transporter Glut1 in human red cells adsorbed on lectin-gel beads. J. Chromatogr. B 739:55–62.
Gu, J.H., Yam, C.M., Li, S., Cai, C.Z. (2004) Nanometric protein arrays on protein-resistant monolayers on silicon
surfaces. J. Am. Chem. Soc. 126:8098–8099.
Guellouz, A., Valerio-Lepiniec, M., Urvoas, A., Chevrel, A., Graille, M., Fourati-Kammoun, Z., Desmadril, M., van
Tilbeurgh, H., Minard, P. (2013) Selection of specific protein binders for pre-defined targets from an optimized
library of artificial helicoidal repeat proteins (alphaRep). PLoS One 8:e71512.
Harding, P.J., Hadingham, T.C., McDonnell, J.M., Watts, A. (2006) Direct analysis of a GPCR-agonist interaction by
surface plasmon resonance. Eur. Biophys. J. Biophys. Lett. 35:709–712.
Hochuli, E., Döbeli, H., Schacher, A. (1987) New metal chelate adsorbent selective for proteins and peptides containing
neighbouring histidine residues. J. Chromatogr. 411:177–184.
Hoff, J.D., Cheng, L.J., Meyhofer, E., Guo, L.J., Hunt, A.J. (2004) Nanoscale protein patterning by imprint lithography.
Nano Lett. 4:853–857.
Hong, Y.L., Webb, B.L., Su, H., Mozdy, E.J., Fang, Y., Wu, Q., Liu, L., Beck, J., Ferrie, A.M., Raghavan, S., Mauro, J.,
Carre, A., Mueller, D., Lai, F., Rasnow, B., Johnson, M., Min, H.S., Salon, J., Lahiri, J. (2005) Functional GPCR
microarrays. J. Am. Chem. Soc. 127:15350–15351.
Hoover, D.K., Lee, E.J., Chan, E.W.L., Yousaf, M.N. (2007) Electroactive nanoarrays for biospecific ligand mediated
studies of cell adhesion. ChemBioChem 8:1920–1923.
620
13 Amphipol-Mediated Immobilization of Membrane Proteins and Its Applications
Submicron streptavidin patterns for protein assembly. Langmuir 22:7444–7450.
Cosnier, S., Galland, B., Gondran, C., Le Pellec, A. (1998) Electrogeneration of biotinylated functionalized polypyrroles
for the simple immobilization of enzymes. Electroanalysis 10:808–813.
Cosnier, S., Stoytcheva, M., Senillou, A., Perrot, H., Furriel, R.P.M., Leone, F.A. (1999) A biotinylated conducting
polypyrrole for the spatially controlled construction of an amperometric biosensor. Anal. Chem. 71:3692–3697.
Coyer, S.R., García, A.J., Delamarche, E. (2007) Facile preparation of complex protein architectures with sub-100-nm
resolution on surfaces. Angew. Chem. Int. Ed. 46:6837–6840.
Dahmane, T., Damian, M., Mary, S., Popot, J.-L., Banères, J.-L. (2009) Amphipol-assisted in vitro folding of G proteincoupled receptors. Biochemistry 48:6516–6521.
Darmanin, T., Bellanger, H., Guittard, F., Lisboa, P., Zurn, M., Colpo, P., Gilliland, D., Rossi, F. (2012) Structured
biotinylated poly(3,4-ethylenedioxypyrrole) electrodes for biochemical applications. RSC Adv. 2:1033–1039.
Della Pia, E.A., Holm, J., Lloret, N., Le Bon, C., Popot, J.-L., Zoonens, M., Nygård, J., Martinez, K.L. (2014a) A step
closer to membrane protein multiplexed nano-arrays using biotin-doped polypyrrole. ACS Nano 8:1844–1853.
Della Pia, E.A., Westh Hansen, R., Zoonens, M., Martinez, K.L. (2014b) Functionalized amphipols: A versatile toolbox
suitable for applications of membrane proteins in synthetic biology. J. Membr. Biol. 247:815–826.
Fang, Y., Frutos, A.G., Lahiri, J. (2002) Membrane protein microarrays. J. Am. Chem. Soc. 124:2394–2395.
Fernandez, A., Le Bon, C., Baumlin, N., Giusti, F., Crémel, G., Popot, J.-L., Bagnard, D. (2014) In vivo characterization
of the biodistribution profile of amphipols J. Membr. Biol. 247:1043–1051.
Ferrandez, Y., Dezi, M., Bosco, M., Urvoas, A., Valério, M., Le Bon, C., Giusti, F., Broutin, I., Durand, G., Polidori, A.,
Popot, J.-L., Picard, M., Minard, P. (2014) Amphipol-mediated screening of molecular orthoses specific for
membrane protein targets. J. Membr. Biol. 247:925–940.
Franklin, G.I., Potter, L.T. (1972) Studies of the binding of α-bungarotoxin to membrane-bound and detergent-dispersed
acetylcholine receptors from Torpedo electric tissue. FEBS Lett. 28:101–106.
Friedrich, M.G., Giess, F., Naumann, R., Knoll, W., Ataka, K., Heberle, J., Hrabakova, J., Murgida, D.H., Hildebrandt,
P. (2004) Active site structure and redox processes of cytochrome c oxidase immobilised in a novel biomimetic lipid
membrane on an electrode. Chem. Commun. 21:2376–2377.
Früh, V., IJzerman, A.P., Siegal, G. (2011) How to catch a membrane protein in action: a review of functional membrane
protein immobilization strategies and their applications. Chem. Rev. 111:640–656.
Giusti, F., Kessler, P., Westh Hansen, R., Della Pia, E.A., Le Bon, C., Mourier, G., Popot, J.-L., Martinez, K.L., Zoonens,
M. (2015) Synthesis of a polyhistidine-bearing amphipol and its use for immobilizing membrane proteins. Biomacromolecules 16:3751–3761.
Giusti, F., Rieger, J., Catoire, L., Qian, S., Calabrese, A.N., Watkinson, T.G., Casiraghi, M., Radford, S.E., Ashcroft, A.
E., Popot, J.-L. (2014) Synthesis, characterization and applications of a perdeuterated amphipol. J. Membr. Biol.
247:909–924.
Gohon, Y., Giusti, F., Prata, C., Charvolin, D., Timmins, P., Ebel, C., Tribet, C., Popot, J.-L. (2006) Well-defined
nanoparticles formed by hydrophobic assembly of a short and polydisperse random terpolymer, amphipol A8-35.
Langmuir 22:1281–1290.
Gohon, Y., Pavlov, G., Timmins, P., Tribet, C., Popot, J.-L., Ebel, C. (2004) Partial specific volume and solvent
interactions of amphipol A8-35. Anal. Biochem. 334:318–334.
Gottschalk, I., Li, Y.M., Lundahl, P. (2000) Chromatography on cells: analyses of solute interactions with the glucose
transporter Glut1 in human red cells adsorbed on lectin-gel beads. J. Chromatogr. B 739:55–62.
Gu, J.H., Yam, C.M., Li, S., Cai, C.Z. (2004) Nanometric protein arrays on protein-resistant monolayers on silicon
surfaces. J. Am. Chem. Soc. 126:8098–8099.
Guellouz, A., Valerio-Lepiniec, M., Urvoas, A., Chevrel, A., Graille, M., Fourati-Kammoun, Z., Desmadril, M., van
Tilbeurgh, H., Minard, P. (2013) Selection of specific protein binders for pre-defined targets from an optimized
library of artificial helicoidal repeat proteins (alphaRep). PLoS One 8:e71512.
Harding, P.J., Hadingham, T.C., McDonnell, J.M., Watts, A. (2006) Direct analysis of a GPCR-agonist interaction by
surface plasmon resonance. Eur. Biophys. J. Biophys. Lett. 35:709–712.
Hochuli, E., Döbeli, H., Schacher, A. (1987) New metal chelate adsorbent selective for proteins and peptides containing
neighbouring histidine residues. J. Chromatogr. 411:177–184.
Hoff, J.D., Cheng, L.J., Meyhofer, E., Guo, L.J., Hunt, A.J. (2004) Nanoscale protein patterning by imprint lithography.
Nano Lett. 4:853–857.
Hong, Y.L., Webb, B.L., Su, H., Mozdy, E.J., Fang, Y., Wu, Q., Liu, L., Beck, J., Ferrie, A.M., Raghavan, S., Mauro, J.,
Carre, A., Mueller, D., Lai, F., Rasnow, B., Johnson, M., Min, H.S., Salon, J., Lahiri, J. (2005) Functional GPCR
microarrays. J. Am. Chem. Soc. 127:15350–15351.
Hoover, D.K., Lee, E.J., Chan, E.W.L., Yousaf, M.N. (2007) Electroactive nanoarrays for biospecific ligand mediated
studies of cell adhesion. ChemBioChem 8:1920–1923.
620
13 Amphipol-Mediated Immobilization of Membrane Proteins and Its Applications