Schulz, G.E. (2011) A new classification of membrane protein crystals. J. Mol. Biol. 407:640–646.
Sennhauser, G., Grütter, M.G. (2008) Chaperone-assisted crystallography with DARPins. Structure 16:1443–1453.
Serrano-Vega, M.J., Magnani, F., Shibata, Y., Tate, C.G. (2008) Conformational thermostabilization of the β 1 -adrenergic
receptor in a detergent-resistant form. Proc. Natl. Acad. Sci. USA 105:877–882.
Sharma, K.S., Durand, G., Gabel, F., Bazzacco, P., Le Bon, C., Billon-Denis, E., Catoire, L.J., Popot, J.-L., Ebel, C.,
Pucci, B. (2012) Non-ionic amphiphilic homopolymers: Synthesis, solution properties, and biochemical validation.
Langmuir 28:4625–4639.
Shibata, Y., White, J.F., Serrano-Vega, M.J., Magnani, F., Aloia, A.L., Grisshammer, R., Tate, C.G. (2009) Thermostabilization of the neurotensin receptor NTS1. J. Mol. Biol. 390:262–277.
Smith, A.L. (1967) Preparation, properties, and conditions for assay of mitochondria: slaughterhouse material, small
scale. Methods Enzymol. 10:81–86.
Suga, M., Akita, F., Sugahara, M., Kubo, M., Nakajima, Y., Nakane, T., Yamashita, K., Umena, Y., Nakabayashi, M.,
Yamane, T., Nakano, T., Suzuki, M., Masuda, T., Inoue, S., Kimura, T., Nomura, T., Yonekura, S., Yu, L.J.,
Sakamoto, T., Motomura, T., Chen, J.H., Kato, Y., Noguchi, T., Tono, K., Joti, Y., Kameshima, T., Hatsui, T.,
Nango, E., Tanaka, R., Naitow, H., Matsuura, Y., Yamashita, A., Yamamoto, M., Nureki, O., Yabashi, M., Ishikawa,
T., Iwata, S., Shen, J.R. (2017) Light-induced structural changes and the site of O¼O bond formation in PSII caught
by XFEL. Nature 543:131–135.
Takeda, K., Sato, H., Hino, T., Kono, M., Fukuda, K., Sakurai, I., Okada, T., Kouyama, T. (1998) A novel threedimensional crystal of bacteriorhodopsin obtained by successive fusion of the vesicular assemblies. J. Mol. Biol.
283:463–474.
Tanford, C. (1980) The Hydrophobic Effect: Formation of Micelles and Biological Membranes. 2nd ed., John Wiley &
Sons, New York, 233 p.
Tate, C.G. (2010) Practical considerations of membrane protein instability for purification and crystallisation, in:
Mus-Veteau, I., ed., Membrane Protein Expression. The Humana Press, Totowa, New Jersey, USA, pp. 187–203.
Tribet, C., Audebert, R., Popot, J.-L. (1996) Amphipols: polymers that keep membrane proteins soluble in aqueous
solutions. Proc. Natl. Acad. Sci. USA 93:15047–15050.
Tribet, C., Audebert, R., Popot, J.-L. (1997) Stabilization of hydrophobic colloidal dispersions in water with amphiphilic
polymers: Application to integral membrane proteins. Langmuir 13:5570–5576.
Tribet, C., Diab, C., Dahmane, T., Zoonens, M., Popot, J.-L., Winnik, F.M. (2009) Thermodynamic characterization of
the exchange of detergents and amphipols at the surfaces of integral membrane proteins. Langmuir 25:12623–12634.
Ujwal, R., Bowie, J.U. (2011) Crystallizing membrane proteins using lipidic bicelles. Methods 55:337–341.
Ujwal, R., Cascio, D., Colletier, J.-P., Faham, S., Zhang, J., Toro, L., Ping, P., Abramson, J. (2008) The crystal structure
of mouse VDAC1 at 2.3 Å resolution reveals mechanistic insights into metabolite gating. Proc. Natl. Acad. Sci. USA
105:17742–17747.
Urvoas, A., Valerio-Lepiniec, M., Minard, P. (2012) Artificial proteins from combinatorial approaches. Trends
Biotechnol. 30:512–520.
Uysal, S., Vásquez, V., Tereshko, V., Esaki, K., Fellouse, F.A., Sidhu, S.S., Koide, S., Perozo, E., Kossiakoff, A. (2009)
Crystal structure of full-length KcsA in its closed conformation. Proc. Natl. Acad. Sci. USA 106:6644–6649.
Vaidehi, N., Grisshammer, R., Tate, C.G. (2016) How can mutations thermostabilize G protein-coupled receptors?
Trends Pharmacol. Sci. 37:37–46.
Vinothkumar, K.R. (2011) Structure of rhomboid protease in a lipid environment. J. Mol. Biol. 407:232–247.
Wadsten, P., Wöhri, A.B., Snijder, A., Katona, G., Gardiner, A.T., Cogdell, R.J., Neutze, R., Engström, S. (2006) Lipidic
sponge phase crystallization of membrane proteins. J. Mol. Biol. 364:44–53.
Wiener, M.C. (2001) Existing and emergent roles for surfactants in the three-dimensional crystallization of integral
membrane proteins. Curr. Opin. Colloid Interface Sci. 6:412–419.
Wiener, M.C. (2004) A pedestrian guide to membrane protein crystallization. Methods 34:364–372.
Wöhri, A.B., Johansson, L.C., Wadsten-Hindrichsen, P., Wahlgren, W.Y., Fischer, G., Horsefield, R., Katona, G.,
Nyblom, M., Oberg, F., Young, G., Cogdell, R.J., Fraser, N.J., Engström, S., Neutze, R. (2008) A lipidic-sponge
phase screen for membrane protein crystallization. Structure 16:1003–1009.
Zhang, Q., Tao, H., Hong, W.-X. (2011) New amphiphiles for membrane protein structural biology. Methods
55:318–323.
Zhang, Z., Huang, L.-S., Shulmeister, V.M., Chi, Y.-I., Kim, K.K., Hung, L.-W., Crofts, A.R., Berry, E.A., Kim, S.-H.
(1998) Electron transfer by domain movement in cytochrome bc 1 . Nature 392:677–684.
Zhou, Y., Bowie, J.U. (2000) Building a thermostable membrane protein. J. Biol. Chem. 275:6975–6979.
Zhou, Y., Morais-Cabral, J.H., Kaufman, A., MacKinnon, R. (2001) Chemistry of ion coordination and hydration
revealed by a K
+ channel-F ab complex at 2.0 Å resolution. Nature 414:43–48.
Zoonens, M., Catoire, L.J., Giusti, F., Popot, J.-L. (2005) NMR study of a membrane protein in detergent-free aqueous
solution. Proc. Natl. Acad. Sci. USA 102:8893–8898.
530
11 Amphipols and Membrane Protein Crystallization
Sennhauser, G., Grütter, M.G. (2008) Chaperone-assisted crystallography with DARPins. Structure 16:1443–1453.
Serrano-Vega, M.J., Magnani, F., Shibata, Y., Tate, C.G. (2008) Conformational thermostabilization of the β 1 -adrenergic
receptor in a detergent-resistant form. Proc. Natl. Acad. Sci. USA 105:877–882.
Sharma, K.S., Durand, G., Gabel, F., Bazzacco, P., Le Bon, C., Billon-Denis, E., Catoire, L.J., Popot, J.-L., Ebel, C.,
Pucci, B. (2012) Non-ionic amphiphilic homopolymers: Synthesis, solution properties, and biochemical validation.
Langmuir 28:4625–4639.
Shibata, Y., White, J.F., Serrano-Vega, M.J., Magnani, F., Aloia, A.L., Grisshammer, R., Tate, C.G. (2009) Thermostabilization of the neurotensin receptor NTS1. J. Mol. Biol. 390:262–277.
Smith, A.L. (1967) Preparation, properties, and conditions for assay of mitochondria: slaughterhouse material, small
scale. Methods Enzymol. 10:81–86.
Suga, M., Akita, F., Sugahara, M., Kubo, M., Nakajima, Y., Nakane, T., Yamashita, K., Umena, Y., Nakabayashi, M.,
Yamane, T., Nakano, T., Suzuki, M., Masuda, T., Inoue, S., Kimura, T., Nomura, T., Yonekura, S., Yu, L.J.,
Sakamoto, T., Motomura, T., Chen, J.H., Kato, Y., Noguchi, T., Tono, K., Joti, Y., Kameshima, T., Hatsui, T.,
Nango, E., Tanaka, R., Naitow, H., Matsuura, Y., Yamashita, A., Yamamoto, M., Nureki, O., Yabashi, M., Ishikawa,
T., Iwata, S., Shen, J.R. (2017) Light-induced structural changes and the site of O¼O bond formation in PSII caught
by XFEL. Nature 543:131–135.
Takeda, K., Sato, H., Hino, T., Kono, M., Fukuda, K., Sakurai, I., Okada, T., Kouyama, T. (1998) A novel threedimensional crystal of bacteriorhodopsin obtained by successive fusion of the vesicular assemblies. J. Mol. Biol.
283:463–474.
Tanford, C. (1980) The Hydrophobic Effect: Formation of Micelles and Biological Membranes. 2nd ed., John Wiley &
Sons, New York, 233 p.
Tate, C.G. (2010) Practical considerations of membrane protein instability for purification and crystallisation, in:
Mus-Veteau, I., ed., Membrane Protein Expression. The Humana Press, Totowa, New Jersey, USA, pp. 187–203.
Tribet, C., Audebert, R., Popot, J.-L. (1996) Amphipols: polymers that keep membrane proteins soluble in aqueous
solutions. Proc. Natl. Acad. Sci. USA 93:15047–15050.
Tribet, C., Audebert, R., Popot, J.-L. (1997) Stabilization of hydrophobic colloidal dispersions in water with amphiphilic
polymers: Application to integral membrane proteins. Langmuir 13:5570–5576.
Tribet, C., Diab, C., Dahmane, T., Zoonens, M., Popot, J.-L., Winnik, F.M. (2009) Thermodynamic characterization of
the exchange of detergents and amphipols at the surfaces of integral membrane proteins. Langmuir 25:12623–12634.
Ujwal, R., Bowie, J.U. (2011) Crystallizing membrane proteins using lipidic bicelles. Methods 55:337–341.
Ujwal, R., Cascio, D., Colletier, J.-P., Faham, S., Zhang, J., Toro, L., Ping, P., Abramson, J. (2008) The crystal structure
of mouse VDAC1 at 2.3 Å resolution reveals mechanistic insights into metabolite gating. Proc. Natl. Acad. Sci. USA
105:17742–17747.
Urvoas, A., Valerio-Lepiniec, M., Minard, P. (2012) Artificial proteins from combinatorial approaches. Trends
Biotechnol. 30:512–520.
Uysal, S., Vásquez, V., Tereshko, V., Esaki, K., Fellouse, F.A., Sidhu, S.S., Koide, S., Perozo, E., Kossiakoff, A. (2009)
Crystal structure of full-length KcsA in its closed conformation. Proc. Natl. Acad. Sci. USA 106:6644–6649.
Vaidehi, N., Grisshammer, R., Tate, C.G. (2016) How can mutations thermostabilize G protein-coupled receptors?
Trends Pharmacol. Sci. 37:37–46.
Vinothkumar, K.R. (2011) Structure of rhomboid protease in a lipid environment. J. Mol. Biol. 407:232–247.
Wadsten, P., Wöhri, A.B., Snijder, A., Katona, G., Gardiner, A.T., Cogdell, R.J., Neutze, R., Engström, S. (2006) Lipidic
sponge phase crystallization of membrane proteins. J. Mol. Biol. 364:44–53.
Wiener, M.C. (2001) Existing and emergent roles for surfactants in the three-dimensional crystallization of integral
membrane proteins. Curr. Opin. Colloid Interface Sci. 6:412–419.
Wiener, M.C. (2004) A pedestrian guide to membrane protein crystallization. Methods 34:364–372.
Wöhri, A.B., Johansson, L.C., Wadsten-Hindrichsen, P., Wahlgren, W.Y., Fischer, G., Horsefield, R., Katona, G.,
Nyblom, M., Oberg, F., Young, G., Cogdell, R.J., Fraser, N.J., Engström, S., Neutze, R. (2008) A lipidic-sponge
phase screen for membrane protein crystallization. Structure 16:1003–1009.
Zhang, Q., Tao, H., Hong, W.-X. (2011) New amphiphiles for membrane protein structural biology. Methods
55:318–323.
Zhang, Z., Huang, L.-S., Shulmeister, V.M., Chi, Y.-I., Kim, K.K., Hung, L.-W., Crofts, A.R., Berry, E.A., Kim, S.-H.
(1998) Electron transfer by domain movement in cytochrome bc 1 . Nature 392:677–684.
Zhou, Y., Bowie, J.U. (2000) Building a thermostable membrane protein. J. Biol. Chem. 275:6975–6979.
Zhou, Y., Morais-Cabral, J.H., Kaufman, A., MacKinnon, R. (2001) Chemistry of ion coordination and hydration
revealed by a K
+ channel-F ab complex at 2.0 Å resolution. Nature 414:43–48.
Zoonens, M., Catoire, L.J., Giusti, F., Popot, J.-L. (2005) NMR study of a membrane protein in detergent-free aqueous
solution. Proc. Natl. Acad. Sci. USA 102:8893–8898.
530
11 Amphipols and Membrane Protein Crystallization