Popot, J.-L., Althoff, T., Bagnard, D., Banères, J.-L., Bazzacco, P., Billon-Denis, E., Catoire, L.J., Champeil, P.,
Charvolin, D., Cocco, M.J., Crémel, G., Dahmane, T., de la Maza, L.M., Ebel, C., Gabel, F., Giusti, F., Gohon,
Y., Goormaghtigh, E., Guittet, E., Kleinschmidt, J.H., Kühlbrandt, W., Le Bon, C., Martinez, K.L., Picard, M., Pucci,
B., Rappaport, F., Sachs, J.N., Tribet, C., van Heijenoort, C., Wien, F., Zito, F., Zoonens, M. (2011) Amphipols from
A to Z. Annu. Rev. Biophys. 40:379–408.
Proverbio, D., Roos, C., Beyermann, M., Orbán, E., Dötsch, V., Bernhard, F. (2013) Functional properties of cell-free
expressed human endothelin A and endothelin B receptors in artificial membrane environments. Biochim. Biophys.
Acta 1828:2182–2192.
Proverbio, D., Henrich, E., Orbán, E., Dötsch, V., Bernhard, F. (2014) Membrane protein quality control in cell-free
expression systems: Tools, strategies and case studies, in: Mus-Veteau, I., ed., Membrane Proteins Production for
Structural Analysis. Springer, New York, pp. 45–70.
Rajesh, S., Knowles, T.J., Overduin, M. (2011) Production of membrane proteins without cells or detergents. New
Biotechnol. 28:250–254.
Reckel, S., Gottstein, G., Stehle, J., Löhr, F., Verhoefen, M.-K., Takeda, M., Silvers, R., Kainosho, M., Glaubitz, C.,
Wachtveitl, J., Bernhard, F., Schwalbe, H., Güntert, P.G., Dötsch, V. (2011) Solution NMR structure of
proteorhodopsin. Angew. Chem. Int. Ed. 50:11942–11946.
Rues, R.-B., Orbán, E., Dötsch, V., Bernhard, F. (2014) Cell-free expression of G protein-coupled receptors: new
pipelines for challenging targets. Biol. Chem. 395:1425–1434.
Rues, R.-B., Henrich, E., Boland, C., Caffrey, M., Bernhard, F. (2016) Cell-free production of membrane proteins in
Escherichia coli lysates for functional and structural studies. Methods Mol. Biol. 1432:1–21.
Sachse, R., Dondapati, S.K., Fenz, S.F., Schmidt, T., Kubick, S. (2014) Membrane protein synthesis in cell-free systems:
From bio-mimetic systems to bio-membranes. FEBS Lett. 588:2774–2781.
Shadiac, N., Nagarajan, Y., Waters, S., Hrmova, M. (2013) Close allies in membrane protein research: Cell-free synthesis
and nanotechnology. Mol. Membr. Biol. 30:229–245.
Sharma, K.S., Durand, G., Gabel, F., Bazzacco, P., Le Bon, C., Billon-Denis, E., Catoire, L.J., Popot, J.-L., Ebel, C.,
Pucci, B. (2012) Non-ionic amphiphilic homopolymers: Synthesis, solution properties, and biochemical validation.
Langmuir 28:4625–4639.
Shenkarev, Z.O., Lyukmanova, E.N., Butenko, I.O., Petrovskaya, L.E., Paramonov, A.S., Shulepko, M.A., Nekrasova,
O.V., Kirpichnikov, M.P., Arseniev, A.S. (2013) Lipid-protein nanodiscs promote in vitro folding of transmembrane
domains of multi-helical and multimeric membrane proteins. Biochim. Biophys. Acta 1828:776–784.
Shirokov, V.A., Kommer, A., Kolb, V.A., Spirin, A.S. (2007) Continuous-exchange protein-synthesizing systems.
Methods Mol. Biol. 375:19–55.
Sonar, S., Patel, N., Fischer, W., Rothschild, K.J. (1993) Cell-free synthesis, functional refolding, and spectroscopic
characterization of bacteriorhodopsin, an integral membrane protein. Biochemistry 32:13777–13781.
Spirin, A.S., Baranov, V.I., Ryabova, L., Ovodov, S.Y., Alakhov, Y.B. (1988) A continuous cell-free translation system
capable of producing polypeptides in high yield. Science 242:1162–1164.
Tu, D., Blaha, G., Moore, P.B., Steitz, T.A. (2005) Structures of MLS B K antibiotics bound to mutated large ribosomal
subunits provide a structural explanation for resistance. Cell 121:257–270.
Uhlemann, E.M., Pierson, H.E., Fillingame, R.H., Dmitriev, O.Y. (2012) Cell-free synthesis of membrane subunits of
ATP synthase in phospholipid bicelles: NMR shows subunit fold similar to the protein in the cell membrane. Prot.
Sci. 21:279–288.
Van Gelder, P., De Cock, H., Tommassen, J. (1994) Detergent-induced folding of the outer-membrane protein PhoE, a
pore protein induced by phosphate limitation. Eur. J. Biochem. 226:783–787.
Wada, T., Shimono, K., Kikukawa, T., Hato, M., Shinya, N., Kim, S.Y., Kimura-Someya, T., Shirouzu, M., Tamogami,
J., Miyauchi, S., Jung, K.H., Kamo, N., Yokoyama, S. (2011) Crystal structure of the eukaryotic light-driven protonpumping rhodopsin, Acetabularia rhodopsin II, from marine alga. J. Mol. Biol. 411:986–998.
Wang, X.Q., Corin, K., Baaske, P., Wienken, C.J., Jerabek-Willemsen, M., Duhr, S., Braun, D., Zhang, S.G. (2011)
Peptide surfactants for cell-free production of functional G protein-coupled receptors. Proc. Natl. Acad. Sci. USA
108:9049–9054.
Wuu, J.J., Swartz, J.R. (2008) High yield cell-free production of integral membrane proteins without refolding or
detergents. Biochim. Biophys. Acta 1778:1237–1250.
Zemella, A., Thoring, L., Hoffmeister, C., Kubick, S. (2015) Cell-free protein synthesis: Pros and cons of prokaryotic and
eukaryotic systems. ChemBioChem 16:2420–2431.
Zoonens, M., Popot, J.-L. (2014) Amphipols for each season. J. Membr. Biol. 247:759–796.
Zoonens, M., Zito, F., Martinez, K.L., Popot, J.-L. (2014) Amphipols: a general introduction and some protocols, in:
Mus-Veteau, I., ed., Membrane Proteins Production for Structural Analysis. Springer, New York, Heidelberg,
Dordrecht, London, pp. 173–203.
Zubay, G. (1973) In vitro synthesis of protein in microbial systems. Annu. Rev. Genet. 7:267–287.
References
379
Charvolin, D., Cocco, M.J., Crémel, G., Dahmane, T., de la Maza, L.M., Ebel, C., Gabel, F., Giusti, F., Gohon,
Y., Goormaghtigh, E., Guittet, E., Kleinschmidt, J.H., Kühlbrandt, W., Le Bon, C., Martinez, K.L., Picard, M., Pucci,
B., Rappaport, F., Sachs, J.N., Tribet, C., van Heijenoort, C., Wien, F., Zito, F., Zoonens, M. (2011) Amphipols from
A to Z. Annu. Rev. Biophys. 40:379–408.
Proverbio, D., Roos, C., Beyermann, M., Orbán, E., Dötsch, V., Bernhard, F. (2013) Functional properties of cell-free
expressed human endothelin A and endothelin B receptors in artificial membrane environments. Biochim. Biophys.
Acta 1828:2182–2192.
Proverbio, D., Henrich, E., Orbán, E., Dötsch, V., Bernhard, F. (2014) Membrane protein quality control in cell-free
expression systems: Tools, strategies and case studies, in: Mus-Veteau, I., ed., Membrane Proteins Production for
Structural Analysis. Springer, New York, pp. 45–70.
Rajesh, S., Knowles, T.J., Overduin, M. (2011) Production of membrane proteins without cells or detergents. New
Biotechnol. 28:250–254.
Reckel, S., Gottstein, G., Stehle, J., Löhr, F., Verhoefen, M.-K., Takeda, M., Silvers, R., Kainosho, M., Glaubitz, C.,
Wachtveitl, J., Bernhard, F., Schwalbe, H., Güntert, P.G., Dötsch, V. (2011) Solution NMR structure of
proteorhodopsin. Angew. Chem. Int. Ed. 50:11942–11946.
Rues, R.-B., Orbán, E., Dötsch, V., Bernhard, F. (2014) Cell-free expression of G protein-coupled receptors: new
pipelines for challenging targets. Biol. Chem. 395:1425–1434.
Rues, R.-B., Henrich, E., Boland, C., Caffrey, M., Bernhard, F. (2016) Cell-free production of membrane proteins in
Escherichia coli lysates for functional and structural studies. Methods Mol. Biol. 1432:1–21.
Sachse, R., Dondapati, S.K., Fenz, S.F., Schmidt, T., Kubick, S. (2014) Membrane protein synthesis in cell-free systems:
From bio-mimetic systems to bio-membranes. FEBS Lett. 588:2774–2781.
Shadiac, N., Nagarajan, Y., Waters, S., Hrmova, M. (2013) Close allies in membrane protein research: Cell-free synthesis
and nanotechnology. Mol. Membr. Biol. 30:229–245.
Sharma, K.S., Durand, G., Gabel, F., Bazzacco, P., Le Bon, C., Billon-Denis, E., Catoire, L.J., Popot, J.-L., Ebel, C.,
Pucci, B. (2012) Non-ionic amphiphilic homopolymers: Synthesis, solution properties, and biochemical validation.
Langmuir 28:4625–4639.
Shenkarev, Z.O., Lyukmanova, E.N., Butenko, I.O., Petrovskaya, L.E., Paramonov, A.S., Shulepko, M.A., Nekrasova,
O.V., Kirpichnikov, M.P., Arseniev, A.S. (2013) Lipid-protein nanodiscs promote in vitro folding of transmembrane
domains of multi-helical and multimeric membrane proteins. Biochim. Biophys. Acta 1828:776–784.
Shirokov, V.A., Kommer, A., Kolb, V.A., Spirin, A.S. (2007) Continuous-exchange protein-synthesizing systems.
Methods Mol. Biol. 375:19–55.
Sonar, S., Patel, N., Fischer, W., Rothschild, K.J. (1993) Cell-free synthesis, functional refolding, and spectroscopic
characterization of bacteriorhodopsin, an integral membrane protein. Biochemistry 32:13777–13781.
Spirin, A.S., Baranov, V.I., Ryabova, L., Ovodov, S.Y., Alakhov, Y.B. (1988) A continuous cell-free translation system
capable of producing polypeptides in high yield. Science 242:1162–1164.
Tu, D., Blaha, G., Moore, P.B., Steitz, T.A. (2005) Structures of MLS B K antibiotics bound to mutated large ribosomal
subunits provide a structural explanation for resistance. Cell 121:257–270.
Uhlemann, E.M., Pierson, H.E., Fillingame, R.H., Dmitriev, O.Y. (2012) Cell-free synthesis of membrane subunits of
ATP synthase in phospholipid bicelles: NMR shows subunit fold similar to the protein in the cell membrane. Prot.
Sci. 21:279–288.
Van Gelder, P., De Cock, H., Tommassen, J. (1994) Detergent-induced folding of the outer-membrane protein PhoE, a
pore protein induced by phosphate limitation. Eur. J. Biochem. 226:783–787.
Wada, T., Shimono, K., Kikukawa, T., Hato, M., Shinya, N., Kim, S.Y., Kimura-Someya, T., Shirouzu, M., Tamogami,
J., Miyauchi, S., Jung, K.H., Kamo, N., Yokoyama, S. (2011) Crystal structure of the eukaryotic light-driven protonpumping rhodopsin, Acetabularia rhodopsin II, from marine alga. J. Mol. Biol. 411:986–998.
Wang, X.Q., Corin, K., Baaske, P., Wienken, C.J., Jerabek-Willemsen, M., Duhr, S., Braun, D., Zhang, S.G. (2011)
Peptide surfactants for cell-free production of functional G protein-coupled receptors. Proc. Natl. Acad. Sci. USA
108:9049–9054.
Wuu, J.J., Swartz, J.R. (2008) High yield cell-free production of integral membrane proteins without refolding or
detergents. Biochim. Biophys. Acta 1778:1237–1250.
Zemella, A., Thoring, L., Hoffmeister, C., Kubick, S. (2015) Cell-free protein synthesis: Pros and cons of prokaryotic and
eukaryotic systems. ChemBioChem 16:2420–2431.
Zoonens, M., Popot, J.-L. (2014) Amphipols for each season. J. Membr. Biol. 247:759–796.
Zoonens, M., Zito, F., Martinez, K.L., Popot, J.-L. (2014) Amphipols: a general introduction and some protocols, in:
Mus-Veteau, I., ed., Membrane Proteins Production for Structural Analysis. Springer, New York, Heidelberg,
Dordrecht, London, pp. 173–203.
Zubay, G. (1973) In vitro synthesis of protein in microbial systems. Annu. Rev. Genet. 7:267–287.
References
379