Logez, C., Damian, M., Legros, C., Dupré, C., Guéry, M., Mary, S., Wagner, R., M'Kadmi, C., Nosjean, O., Fould, B.,
Marie, J., Fehrentz, J.A., Martinez, J., Ferry, G., Boutin, J.A., Banères, J.-L. (2016) Detergent-free isolation of
functional G protein-coupled receptors into nanometric lipid particles. Biochemistry 55:38–48.
Long, A.R., O’Brien, C.C., Malhotra, K., Schwall, C.T., Albert, A.D., Watts, A., Alder, N.N. (2013) A detergent-free
strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs.
BMC Biotechnol. 13:41.
Lu, P., Bai, X.-C., Ma, D., Xie, T., Yan, C., Sun, L., Yang, G., Zhao, Y., Zhou, R., Scheres, S.H.W., Shi, Y. (2014)
Three-dimensional structure of human γ-secretase. Nature 512:166–170.
Luccardini, C., Tribet, C., Vial, F., Marchi-Artzner, V., Dahan, M. (2006) Size, charge, and interactions with giant lipid
vesicles of quantum dots coated with an amphiphilic macromolecule. Langmuir 22:2304–2310.
Lund, S., Orlowski, S., de Foresta, B., Champeil, P., le Maire, M., Møller, J.V. (1989) Detergent structure and associated
lipid as determinants in the stabilization of solubilized Ca
2+ -ATPase from sarcoplasmic reticulum. J. Biol. Chem.
264:4907–4915.
Ma, D., Martin, N., Tribet, C., Winnik, F.M. (2014) Quantitative characterization by asymmetrical flow field-flow
fractionation of IgG thermal aggregation with and without polymer protective agents. Anal. Bioanal. Chem.
406:7539–7547.
Malhotra, K., Alder, N.N. (2014) Advances in the use of nanoscale bilayers to study membrane protein structure and
function. Biotechnol. Genet. Eng. Rev. 30:79–93.
Marie, E., Sagan, S., Cribier, S., Tribet, C. (2014) Amphiphilic macromolecules on cell membranes: from protective
layers to controlled permeabilization. J. Membr. Biol. 247:861–881.
Martin, N., Ma, D., Herbet, A., Boquet, D., Winnik, F.M., Tribet, C. (2014) Prevention of thermally induced aggregation
of IgG antibodies by noncovalent interaction with poly(acrylate) derivatives. Biomacromolecules 15:2952–2962.
Martin, N., Ruchmann, J., Tribet, C. (2015) Prevention of aggregation during refolding of carbonic anhydrase via
Coulomb and hydrophobic complexation with octadecyl-modified or azobenzene-modified poly(acrylate)
derivatives. Langmuir 31:338–349.
Martinez, K.L., Gohon, Y., Corringer, P.-J., Tribet, C., Mérola, F., Changeux, J.-P., Popot, J.-L. (2002) Allosteric
transitions of Torpedo acetylcholine receptor in lipids, detergent and amphipols: molecular interactions vs. physical
constraints. FEBS Lett. 528:251–256.
Mary, S., Damian, M., Rahmeh, R., Marie, J., Mouillac, B., Banères, J.-L. (2014) Amphipols in G protein-coupled
receptor pharmacology: What are they good for? J. Membr. Biol. 247:853–860.
Merino, J.M., Møller, J.V., Gutiérrez-Merino, C. (1994) Thermal unfolding of monomeric Ca
2+ ,Mg
2+ -ATPase from
sarcoplasmic reticulum of rabbit skeletal muscle. FEBS Lett. 343:155–159.
Milder, S.J., Thorgeirsson, T.E., Miercke, L.J., Stroud, R.M., Kliger, D.S. (1991) Effects of detergent environments on
the photocycle of purified monomeric bacteriorhodopsin. Biochemistry 30:1751–1761.
Møller, J.V., le Maire, M. (1993) Detergent binding as a measure of hydrophobic surface area of integral membrane
proteins. J. Biol. Chem. 268:18659–18672.
Møller, J.V., Olesen, C., Winther, A.M.L., Nissen, P. (2010) The sarcoplasmic Ca
2+ -ATPase: design of a perfect chemiosmotic pump. Q. Rev. Biophys. 43:501–566.
Murakami, S., Nakashima, R., Yamashita, E., Yamaguchi, A. (2002) Crystal structure of bacterial multidrug efflux
transporter AcrB. Nature 419:587–593.
Nagy, J.K., Kuhn Hoffmann, A., Keyes, M.H., Gray, D.N., Oxenoid, K., Sanders, C.R. (2001) Use of amphipathic
polymers to deliver a membrane protein to lipid bilayers. FEBS Lett. 1:115–120.
Ning, Z., Hawley, B., Seebun, D., Figeys, D. (2014) APols-aided protein precipitation: a rapid method for concentrating
proteins for proteomic analysis. J. Membr. Biol. 247:941–947.
Ning, Z., Seebun, D., Hawley, B., Chang, C.-K., Figeys, D. (2013) From cells to peptides: “One-stop” integrated
proteomic processing using amphipols. J. Proteome Res. 12:1512–1519.
Norimatsu, Y., Hasegawa, K., Shimizu, N., Toyoshima, C. (2017) Protein-phospholipid interplay revealed with crystals
of a calcium pump. Nature 545:193–198.
Nowaczyk, M., Oworah-Nkruma, R., Zoonens, M., Rögner, M., Popot, J.-L. (2004) Amphipols: strategies for an
improved PS2 environment in aqueous solution, in: Miyake, J. (Ed.), Biohydrogen III. Elsevier, Dordrecht, The
Netherlands, Kyoto, pp. 151–159.
Obara, K., Miyashita, N., Xu, C., Toyoshima, I., Sugita, Y., Inesi, G., Toyoshima, C. (2005) Structural role of
countertransport revealed in Ca
2+ pump crystal structure in the absence of Ca
2+ . Proc. Natl. Acad. Sci. USA
102:14489–14496.
Oluwole, A.O., Danielczak, B., Meister, A., Babalola, J.O., Vargas, C., Keller, S. (2017) Solubilization of membrane
proteins into functional lipid-bilayer nanodiscs using a diisobutylene/maleic acid copolymer. Angew. Chem. Int.
Ed. Engl. 56:1919–1924.
Opačić, M., Durand, G., Bosco, M., Polidori, A., Popot, J.-L. (2014a) Amphipols and photosynthetic light-harvesting
pigment-protein complexes. J. Membr. Biol. 247:1031–1041.
328
5 Formation and Properties of Membrane Protein/Amphipol Complexes
Marie, J., Fehrentz, J.A., Martinez, J., Ferry, G., Boutin, J.A., Banères, J.-L. (2016) Detergent-free isolation of
functional G protein-coupled receptors into nanometric lipid particles. Biochemistry 55:38–48.
Long, A.R., O’Brien, C.C., Malhotra, K., Schwall, C.T., Albert, A.D., Watts, A., Alder, N.N. (2013) A detergent-free
strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs.
BMC Biotechnol. 13:41.
Lu, P., Bai, X.-C., Ma, D., Xie, T., Yan, C., Sun, L., Yang, G., Zhao, Y., Zhou, R., Scheres, S.H.W., Shi, Y. (2014)
Three-dimensional structure of human γ-secretase. Nature 512:166–170.
Luccardini, C., Tribet, C., Vial, F., Marchi-Artzner, V., Dahan, M. (2006) Size, charge, and interactions with giant lipid
vesicles of quantum dots coated with an amphiphilic macromolecule. Langmuir 22:2304–2310.
Lund, S., Orlowski, S., de Foresta, B., Champeil, P., le Maire, M., Møller, J.V. (1989) Detergent structure and associated
lipid as determinants in the stabilization of solubilized Ca
2+ -ATPase from sarcoplasmic reticulum. J. Biol. Chem.
264:4907–4915.
Ma, D., Martin, N., Tribet, C., Winnik, F.M. (2014) Quantitative characterization by asymmetrical flow field-flow
fractionation of IgG thermal aggregation with and without polymer protective agents. Anal. Bioanal. Chem.
406:7539–7547.
Malhotra, K., Alder, N.N. (2014) Advances in the use of nanoscale bilayers to study membrane protein structure and
function. Biotechnol. Genet. Eng. Rev. 30:79–93.
Marie, E., Sagan, S., Cribier, S., Tribet, C. (2014) Amphiphilic macromolecules on cell membranes: from protective
layers to controlled permeabilization. J. Membr. Biol. 247:861–881.
Martin, N., Ma, D., Herbet, A., Boquet, D., Winnik, F.M., Tribet, C. (2014) Prevention of thermally induced aggregation
of IgG antibodies by noncovalent interaction with poly(acrylate) derivatives. Biomacromolecules 15:2952–2962.
Martin, N., Ruchmann, J., Tribet, C. (2015) Prevention of aggregation during refolding of carbonic anhydrase via
Coulomb and hydrophobic complexation with octadecyl-modified or azobenzene-modified poly(acrylate)
derivatives. Langmuir 31:338–349.
Martinez, K.L., Gohon, Y., Corringer, P.-J., Tribet, C., Mérola, F., Changeux, J.-P., Popot, J.-L. (2002) Allosteric
transitions of Torpedo acetylcholine receptor in lipids, detergent and amphipols: molecular interactions vs. physical
constraints. FEBS Lett. 528:251–256.
Mary, S., Damian, M., Rahmeh, R., Marie, J., Mouillac, B., Banères, J.-L. (2014) Amphipols in G protein-coupled
receptor pharmacology: What are they good for? J. Membr. Biol. 247:853–860.
Merino, J.M., Møller, J.V., Gutiérrez-Merino, C. (1994) Thermal unfolding of monomeric Ca
2+ ,Mg
2+ -ATPase from
sarcoplasmic reticulum of rabbit skeletal muscle. FEBS Lett. 343:155–159.
Milder, S.J., Thorgeirsson, T.E., Miercke, L.J., Stroud, R.M., Kliger, D.S. (1991) Effects of detergent environments on
the photocycle of purified monomeric bacteriorhodopsin. Biochemistry 30:1751–1761.
Møller, J.V., le Maire, M. (1993) Detergent binding as a measure of hydrophobic surface area of integral membrane
proteins. J. Biol. Chem. 268:18659–18672.
Møller, J.V., Olesen, C., Winther, A.M.L., Nissen, P. (2010) The sarcoplasmic Ca
2+ -ATPase: design of a perfect chemiosmotic pump. Q. Rev. Biophys. 43:501–566.
Murakami, S., Nakashima, R., Yamashita, E., Yamaguchi, A. (2002) Crystal structure of bacterial multidrug efflux
transporter AcrB. Nature 419:587–593.
Nagy, J.K., Kuhn Hoffmann, A., Keyes, M.H., Gray, D.N., Oxenoid, K., Sanders, C.R. (2001) Use of amphipathic
polymers to deliver a membrane protein to lipid bilayers. FEBS Lett. 1:115–120.
Ning, Z., Hawley, B., Seebun, D., Figeys, D. (2014) APols-aided protein precipitation: a rapid method for concentrating
proteins for proteomic analysis. J. Membr. Biol. 247:941–947.
Ning, Z., Seebun, D., Hawley, B., Chang, C.-K., Figeys, D. (2013) From cells to peptides: “One-stop” integrated
proteomic processing using amphipols. J. Proteome Res. 12:1512–1519.
Norimatsu, Y., Hasegawa, K., Shimizu, N., Toyoshima, C. (2017) Protein-phospholipid interplay revealed with crystals
of a calcium pump. Nature 545:193–198.
Nowaczyk, M., Oworah-Nkruma, R., Zoonens, M., Rögner, M., Popot, J.-L. (2004) Amphipols: strategies for an
improved PS2 environment in aqueous solution, in: Miyake, J. (Ed.), Biohydrogen III. Elsevier, Dordrecht, The
Netherlands, Kyoto, pp. 151–159.
Obara, K., Miyashita, N., Xu, C., Toyoshima, I., Sugita, Y., Inesi, G., Toyoshima, C. (2005) Structural role of
countertransport revealed in Ca
2+ pump crystal structure in the absence of Ca
2+ . Proc. Natl. Acad. Sci. USA
102:14489–14496.
Oluwole, A.O., Danielczak, B., Meister, A., Babalola, J.O., Vargas, C., Keller, S. (2017) Solubilization of membrane
proteins into functional lipid-bilayer nanodiscs using a diisobutylene/maleic acid copolymer. Angew. Chem. Int.
Ed. Engl. 56:1919–1924.
Opačić, M., Durand, G., Bosco, M., Polidori, A., Popot, J.-L. (2014a) Amphipols and photosynthetic light-harvesting
pigment-protein complexes. J. Membr. Biol. 247:1031–1041.
328
5 Formation and Properties of Membrane Protein/Amphipol Complexes