Production of Lactose-Free Cheese Using
Partially Purified β-Galactosidase
from Enterobacter aerogenes st
KCTC2190
Manisha Maity, D. K. Bhattacharyya, and Jayati Bhowal
Abstract β-galactosidase has numerous applications in milk and other dairy industries including treatment of lactose intolerance. It catalyzes the hydrolysis of milk
sugar lactose into monosaccharides, namely, D-galactose and D-glucose. In this
present study, Enterobacter aerogenes st KCTC2190 was isolated from soil around
cattle shed area, which was capable of producing intracellular β-galactosidase.
Extracted β-galactosidase was partially purified by cold acetone method. After partial
purification, specific activity and purification fold increased to 10.64 ± 1.12 U/mg
and 1.28 fold. Partially purified β-galactosidase was then applied on cheese to
produce lactose-free soft cheese. Physicochemical analysis such as moisture, ash,
fat, carbohydrates, proteins of raw material, control cheese (without enzyme treatment), and cheese from β-galactosidase-treated milk was almost same except lactose
content. This partially purified β-galactosidase displayed hydrolytic effect on cheese
lactose. Lactose hydrolysis of 81.90% occurred after 4 h of treatment of milk with
this partially purified enzyme at 40 °C. After 4 h of enzymatic treatment, lactose
hydrolysis became constant.
Keywords β-galactosidase · Lactose hydrolysis · Cheese
M. Maity · D. K. Bhattacharyya · J. Bhowal (B)
School of Community Science and Technology, Indian Institute of Engineering Science and
Technology, Shibpur, Howrah, West Bengal 711103, India
e-mail: jayatibhowal@gmail.com
M. Maity
e-mail: manisha.maity04@gmail.com
D. K. Bhattacharyya
e-mail: dkb_oiltech@yahoo.co.in
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_5
39
Partially Purified β-Galactosidase
from Enterobacter aerogenes st
KCTC2190
Manisha Maity, D. K. Bhattacharyya, and Jayati Bhowal
Abstract β-galactosidase has numerous applications in milk and other dairy industries including treatment of lactose intolerance. It catalyzes the hydrolysis of milk
sugar lactose into monosaccharides, namely, D-galactose and D-glucose. In this
present study, Enterobacter aerogenes st KCTC2190 was isolated from soil around
cattle shed area, which was capable of producing intracellular β-galactosidase.
Extracted β-galactosidase was partially purified by cold acetone method. After partial
purification, specific activity and purification fold increased to 10.64 ± 1.12 U/mg
and 1.28 fold. Partially purified β-galactosidase was then applied on cheese to
produce lactose-free soft cheese. Physicochemical analysis such as moisture, ash,
fat, carbohydrates, proteins of raw material, control cheese (without enzyme treatment), and cheese from β-galactosidase-treated milk was almost same except lactose
content. This partially purified β-galactosidase displayed hydrolytic effect on cheese
lactose. Lactose hydrolysis of 81.90% occurred after 4 h of treatment of milk with
this partially purified enzyme at 40 °C. After 4 h of enzymatic treatment, lactose
hydrolysis became constant.
Keywords β-galactosidase · Lactose hydrolysis · Cheese
M. Maity · D. K. Bhattacharyya · J. Bhowal (B)
School of Community Science and Technology, Indian Institute of Engineering Science and
Technology, Shibpur, Howrah, West Bengal 711103, India
e-mail: jayatibhowal@gmail.com
M. Maity
e-mail: manisha.maity04@gmail.com
D. K. Bhattacharyya
e-mail: dkb_oiltech@yahoo.co.in
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_5
39