Optimization of Process Parameters
for Biodegradation of Resorcinol
in Presence of Toxic Heavy Metals
by Response Surface Methodology (RSM)
Sonali Hazra Das, Ayimyaba R. Longkumer, Rayanee Chaudhuri,
Srabanti Basu, Sudipta Dey Bandyopadhyay, and Bhaswati Chakraborty
Abstract Environmental pollution is caused by industrial effluents releasing toxic
phenolics like phenol, cresol, resorcinol, etc. along with various heavy metals to the
environment. It is difficult to degrade these pollutants by common soil microflora and
persists for a long time in nature causing environmental pollution and adverse health
conditions. Thus the objective of the present work is to study the biodegradation of
resorcinol by a mixed bacterial culture isolated from the soil near to the outer limit
of effluent discharge of tannery. The mixed bacterial culture was acclimatized to
resorcinol by gradually increasing the concentration of resorcinol in the MS media
to 500 mg/L. A batch kinetic study was carried out for the degradation of 500 mg/L
of resorcinol by the mixed bacterial culture in the presence of different toxic heavy
metals such as Mercury (Hg), Lead (Pb), and Chromium (VI) in concentrations of
5 ppm, 25 ppm, and 50 ppm. Removal efficiency of resorcinol in 20 h in presence
of mercury was 44%, for lead was 68.8%, and for chromium (VI) was 86%. Optimization of the process parameters of the present system was done by Response
Surface Methodology using DesignExpert11.0 software. The various physicochemical parameters viz. aeration (with/without) and concentration of the toxic metals
were optimized for maximum degradation of resorcinol and removal efficiency of
resorcinol. Removal efficiency of resorcinol as obtained under optimized conditions
was 90.82%.
S. H. Das · A. R. Longkumer · R. Chaudhuri · S. Basu · S. D. Bandyopadhyay ·
B. Chakraborty (B)
Department of Biotechnology, Heritage Institute of Technology, Anandapur, Kolkata,
West Bengal 700107, India
e-mail: bhaswati.chakraborty@heritageit.edu
S. H. Das
e-mail: sonalihazra.das@heritageit.edu
S. Basu
e-mail: srabanti.basu@heritageit.edu
S. D. Bandyopadhyay
e-mail: sudipta.dey@heritageit.edu
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_36
359
for Biodegradation of Resorcinol
in Presence of Toxic Heavy Metals
by Response Surface Methodology (RSM)
Sonali Hazra Das, Ayimyaba R. Longkumer, Rayanee Chaudhuri,
Srabanti Basu, Sudipta Dey Bandyopadhyay, and Bhaswati Chakraborty
Abstract Environmental pollution is caused by industrial effluents releasing toxic
phenolics like phenol, cresol, resorcinol, etc. along with various heavy metals to the
environment. It is difficult to degrade these pollutants by common soil microflora and
persists for a long time in nature causing environmental pollution and adverse health
conditions. Thus the objective of the present work is to study the biodegradation of
resorcinol by a mixed bacterial culture isolated from the soil near to the outer limit
of effluent discharge of tannery. The mixed bacterial culture was acclimatized to
resorcinol by gradually increasing the concentration of resorcinol in the MS media
to 500 mg/L. A batch kinetic study was carried out for the degradation of 500 mg/L
of resorcinol by the mixed bacterial culture in the presence of different toxic heavy
metals such as Mercury (Hg), Lead (Pb), and Chromium (VI) in concentrations of
5 ppm, 25 ppm, and 50 ppm. Removal efficiency of resorcinol in 20 h in presence
of mercury was 44%, for lead was 68.8%, and for chromium (VI) was 86%. Optimization of the process parameters of the present system was done by Response
Surface Methodology using DesignExpert11.0 software. The various physicochemical parameters viz. aeration (with/without) and concentration of the toxic metals
were optimized for maximum degradation of resorcinol and removal efficiency of
resorcinol. Removal efficiency of resorcinol as obtained under optimized conditions
was 90.82%.
S. H. Das · A. R. Longkumer · R. Chaudhuri · S. Basu · S. D. Bandyopadhyay ·
B. Chakraborty (B)
Department of Biotechnology, Heritage Institute of Technology, Anandapur, Kolkata,
West Bengal 700107, India
e-mail: bhaswati.chakraborty@heritageit.edu
S. H. Das
e-mail: sonalihazra.das@heritageit.edu
S. Basu
e-mail: srabanti.basu@heritageit.edu
S. D. Bandyopadhyay
e-mail: sudipta.dey@heritageit.edu
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_36
359