Isolation of a Most Potent Bacterial Strain from Soil …
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Table 4 Physicochemical characteristics of the isolated bacterial strain P25
Parameters
Characteristics of P25
1. Ammonia from arginine
+
2. Arginine used as sole source of energy
+
3. Nitrate reduction
+
4. Catalase reduction
−
5. Carbohydrate fermentation
Acidity
Gas formation
a. Fructose
+
+
b. Arabinose
−
+
c. Galactose
+
+
d. Xylose
Not done
Not done
e. Glucose
+
+
f. Lactose
+
+
g. Raffinose
+
+
h. Sucrose
−
+
i. Maltose
+
+
j. Dextrin
+
+
k. Salicin
+
+
l. Mannitol
+
+
m. Glycerol
+
+
n. Inositol
−
+
6. Indole formation
+
7. Litmus milk test
+
8. Starch hydrolysis test
−
9. Urease test
Incubation
10. Voges—Proskauer test
Incubation
11. Growth under anaerobic condition
Not done
12. Growth at different temperatures
+
13. Growth at extreme pH and NaCl concentration
+
4 Conclusion
The isolated most potent bacterial strain was identified as Brevibacillus formosus
strain NRRL NRS-863, which was able to reduce almost 99.44% of phenol. However,
the mechanism of removal of phenol by the isolated strain i.e. whether the strain
degrades it or accumulates it is not known till now. Our further study will reveal it.