Isolation of a Most Potent Bacterial
Strain from Soil for Bioremediation
of Phenol
Debapriya Maity, Pradyut Kundu, and Sunita Adhikari (Nee Pramanik)
Abstract Phenol is present as the basic structural compound of many synthetic
organics. This pollutant generates from different sources such as herbicides, wood
preservatives, petroleum industries, pharmaceuticals, etc. US Environmental Protection Agency marked it as the priority pollutant. The objective of this study is to isolate
a potent bacterial strain from soil which is capable to remove phenol from wastewater.
For this, soil sample was collected from the hospital area and enriched with 500 ppm
phenol for 10 days. After serial dilution of the soil sample, colonies were developed
in petri plate on nutrient agar medium. The isolated colonies were transferred to
the slant and screening was done to select the most potent strain in liquid nutrient
medium containing 500 ppm of phenol. The most efficient strain, P25, was able to
reduce almost ~99.44% of phenol concentration in 24 h, 37 °C temperature, pH 6.8.
The isolated strain was acclimatized in MSM (minimum salt medium) for 2 months
and habituated to remove 700 mg/L phenol concentration. Simultaneously the strain
was biochemically characterized and identified by 16S rDNA sequence analysis.
Keywords Phenol · Pollutant · Minimum salt medium (MSM) · 16S rDNA
D. Maity · S. A. (Nee Pramanik) (B)
Department of Food Technology & Biochemical Engineering, Jadavpur University, Kolkata
700032, India
e-mail: sunitapramanik@gmail.com
D. Maity
e-mail: debapriyamaity552@gmail.com
P. Kundu
Department of Food Processing Technology, Mirmadan Mohanlal Government Polytechnic,
Plassey, Nadia, India
e-mail: kundupradyut@yahoo.co.in
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_29
287
Strain from Soil for Bioremediation
of Phenol
Debapriya Maity, Pradyut Kundu, and Sunita Adhikari (Nee Pramanik)
Abstract Phenol is present as the basic structural compound of many synthetic
organics. This pollutant generates from different sources such as herbicides, wood
preservatives, petroleum industries, pharmaceuticals, etc. US Environmental Protection Agency marked it as the priority pollutant. The objective of this study is to isolate
a potent bacterial strain from soil which is capable to remove phenol from wastewater.
For this, soil sample was collected from the hospital area and enriched with 500 ppm
phenol for 10 days. After serial dilution of the soil sample, colonies were developed
in petri plate on nutrient agar medium. The isolated colonies were transferred to
the slant and screening was done to select the most potent strain in liquid nutrient
medium containing 500 ppm of phenol. The most efficient strain, P25, was able to
reduce almost ~99.44% of phenol concentration in 24 h, 37 °C temperature, pH 6.8.
The isolated strain was acclimatized in MSM (minimum salt medium) for 2 months
and habituated to remove 700 mg/L phenol concentration. Simultaneously the strain
was biochemically characterized and identified by 16S rDNA sequence analysis.
Keywords Phenol · Pollutant · Minimum salt medium (MSM) · 16S rDNA
D. Maity · S. A. (Nee Pramanik) (B)
Department of Food Technology & Biochemical Engineering, Jadavpur University, Kolkata
700032, India
e-mail: sunitapramanik@gmail.com
D. Maity
e-mail: debapriyamaity552@gmail.com
P. Kundu
Department of Food Processing Technology, Mirmadan Mohanlal Government Polytechnic,
Plassey, Nadia, India
e-mail: kundupradyut@yahoo.co.in
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_29
287