Cost-Effective and Eco-Friendly Method
for Decolorization of Malachite Green
by Kocuria marina DAGII
Daiji Brahma and Debjani Dutta
Abstract This study aims for eco-friendly removal of Malachite green (MG) by
‘biosorption’ process. In our study, Kocuria marina DAGII isolated from soil could
effectively decolorize MG approximately up to 99% in continuous study. Column
study was performed for MG removal at different parameters to know the probable
applicability of bacterial cells in industries. Column adsorbent bed was prepared by
immobilizing bacterial cells in alginate. Response surface methodology (RSM) was
performed to screen and optimize different physical parameters to yield maximum
dye removal percentage. Analysis of variance (ANOVA) test was performed to study
the interactive effect of different parameters and adequacy of the quadratic model
in dye removal. From ANOVA test, PreR
2 of 0.9914 and AdjR
2 of 0.9844 obtained
indicated good agreement between experimental and predicted value and generated a reliable quadratic model for MG removal. The final parameters optimized
to get maximum dye removal in continuous mode were bed height 15 cm, flowrate
0.5 ml min
−1 , initial MG concentration 50 mg l
−1 , and alginate concentration of
2.5% (w/v).
Keywords Malachite green · Biosorption · Column study · Response surface
methodology
1 Introduction
Malachite green (MG) is a synthetic triphenylmethane dye which is widely used
in several industries for dyeing purposes (Chen et al. 2011). Excessive amounts of
malachite green in wastewater cause water pollution which affects water transparency
and gas solubility in water. Malachite green can cause severe irritation and pain to
D. Brahma · D. Dutta (B)
Department of Biotechnology, National Institute of Technology Durgapur, MG Avenue, Durgapur,
West Bengal 713209, India
e-mail: debjani.dutta@bt.nitdgp.ac.in; debs_2000in@yahoo.com
D. Brahma
e-mail: daijibrahma.rose@gmail.com
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_28
277
for Decolorization of Malachite Green
by Kocuria marina DAGII
Daiji Brahma and Debjani Dutta
Abstract This study aims for eco-friendly removal of Malachite green (MG) by
‘biosorption’ process. In our study, Kocuria marina DAGII isolated from soil could
effectively decolorize MG approximately up to 99% in continuous study. Column
study was performed for MG removal at different parameters to know the probable
applicability of bacterial cells in industries. Column adsorbent bed was prepared by
immobilizing bacterial cells in alginate. Response surface methodology (RSM) was
performed to screen and optimize different physical parameters to yield maximum
dye removal percentage. Analysis of variance (ANOVA) test was performed to study
the interactive effect of different parameters and adequacy of the quadratic model
in dye removal. From ANOVA test, PreR
2 of 0.9914 and AdjR
2 of 0.9844 obtained
indicated good agreement between experimental and predicted value and generated a reliable quadratic model for MG removal. The final parameters optimized
to get maximum dye removal in continuous mode were bed height 15 cm, flowrate
0.5 ml min
−1 , initial MG concentration 50 mg l
−1 , and alginate concentration of
2.5% (w/v).
Keywords Malachite green · Biosorption · Column study · Response surface
methodology
1 Introduction
Malachite green (MG) is a synthetic triphenylmethane dye which is widely used
in several industries for dyeing purposes (Chen et al. 2011). Excessive amounts of
malachite green in wastewater cause water pollution which affects water transparency
and gas solubility in water. Malachite green can cause severe irritation and pain to
D. Brahma · D. Dutta (B)
Department of Biotechnology, National Institute of Technology Durgapur, MG Avenue, Durgapur,
West Bengal 713209, India
e-mail: debjani.dutta@bt.nitdgp.ac.in; debs_2000in@yahoo.com
D. Brahma
e-mail: daijibrahma.rose@gmail.com
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_28
277