10
S. Chatterjee et al.
research has been accelerated to find ways to convert cellulose into industrial important products. Due to its cellulolytic potential, microbes are the excellent system
for the degradation of cellulose. Therefore, in the present investigation, we have
screened and isolated cellulose-degrading bacteria (CBD) from the soil sample. Out
of 37 bacterial strains, 6 showed cellulose-degrading ability. The cellulase production ability of the six was in the following order—CDB-37 < CDB-32 < CDB-4 <
CDB-29 < CDB-34 < CDB-1. Molecular characterization of CBD-1 showed that the
strain is closely related to Streptomyces species. The isolated CBD-1 bacterial strain
was later used for the saccharification along with S. cerevisiae during fermentation.
After distillation of the fermented mash, the production of alcohol was confirmed
using iodoform test. Thus, the biodiversity of microbes like bacteria, fungi gives us
the chance to identify and isolate strains that could assist in the biological conversion of cellulose into glucose, which can later be used for the production of some
industrial useful products.
Acknowledgements SC, KT, and RSP like to thank GGS Indraprastha University, New Delhi for
all the laboratory space and financial support provided.
Conflict of Interest The authors declare that they have no conflict of interest.
Declaration by authors Appropriate permissions were obtained from responsible authorities for
collecting soil samples for the study from manufacturing industry of Bawana, New Delhi.
References
Balat M (2007) Global bio-fuel processing and production trends. Energ Explor Exploit 25(3):195–
218. https://doi.org/10.1260/014459807782009204
Chun J, Lee JH, Jung Y et al (2007) EzTaxon: a web-based tool for the identification of prokaryotes
based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57(10):2259–2261.
https://doi.org/10.1099/ijs.0.64915-0
Ghosal A, Banerjee S, Chatterjee S (2013) Biofuel precursor from potato waste. Int J Res Eng
Technol 2(3):213–219
Guder DG, Krishna MSR (2019) Isolation and characterization of potential cellulose degrading
bacteria from sheep rumen. J Pure Appl Microbiol 13(3):1831–1839. https://doi.org/10.22207/
jpam.13.3.60
Gupta P, Samant K, Sahu A (2012) Isolation of cellulose-degrading bacteria and determination of
their cellulolytic potential. Int J Microbiol 2012:1–5. https://doi.org/10.1155/2012/578925
Jain M, Gupta AK, Chatterjee S (2014) To optimize the process of alcohol production from banana
peel. Recent Adv Bioenergy Res 3:208–217
Kaur M (2012) Isolation and Screening of cellulose degrading bacteria in kitchen waste and detecting
their degrading potential. IOSR J Mech Civil Eng 1(2):33–35. https://doi.org/10.9790/1684-012
3335
Klemm D, Heublein B, Fink HP et al (2005) Cellulose: fascinating biopolymer and sustainable raw
material. Angew Chem Int Edit 44:3358–3393. https://doi.org/10.1002/anie.200460587
Kumakura M (1997) Preparation of immobilized cellulase beads and their application to hydrolysis of cellulosic materials. Process Biochem 32(7):555–559. https://doi.org/10.1016/s0032-959
2(97)00011-3
S. Chatterjee et al.
research has been accelerated to find ways to convert cellulose into industrial important products. Due to its cellulolytic potential, microbes are the excellent system
for the degradation of cellulose. Therefore, in the present investigation, we have
screened and isolated cellulose-degrading bacteria (CBD) from the soil sample. Out
of 37 bacterial strains, 6 showed cellulose-degrading ability. The cellulase production ability of the six was in the following order—CDB-37 < CDB-32 < CDB-4 <
CDB-29 < CDB-34 < CDB-1. Molecular characterization of CBD-1 showed that the
strain is closely related to Streptomyces species. The isolated CBD-1 bacterial strain
was later used for the saccharification along with S. cerevisiae during fermentation.
After distillation of the fermented mash, the production of alcohol was confirmed
using iodoform test. Thus, the biodiversity of microbes like bacteria, fungi gives us
the chance to identify and isolate strains that could assist in the biological conversion of cellulose into glucose, which can later be used for the production of some
industrial useful products.
Acknowledgements SC, KT, and RSP like to thank GGS Indraprastha University, New Delhi for
all the laboratory space and financial support provided.
Conflict of Interest The authors declare that they have no conflict of interest.
Declaration by authors Appropriate permissions were obtained from responsible authorities for
collecting soil samples for the study from manufacturing industry of Bawana, New Delhi.
References
Balat M (2007) Global bio-fuel processing and production trends. Energ Explor Exploit 25(3):195–
218. https://doi.org/10.1260/014459807782009204
Chun J, Lee JH, Jung Y et al (2007) EzTaxon: a web-based tool for the identification of prokaryotes
based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57(10):2259–2261.
https://doi.org/10.1099/ijs.0.64915-0
Ghosal A, Banerjee S, Chatterjee S (2013) Biofuel precursor from potato waste. Int J Res Eng
Technol 2(3):213–219
Guder DG, Krishna MSR (2019) Isolation and characterization of potential cellulose degrading
bacteria from sheep rumen. J Pure Appl Microbiol 13(3):1831–1839. https://doi.org/10.22207/
jpam.13.3.60
Gupta P, Samant K, Sahu A (2012) Isolation of cellulose-degrading bacteria and determination of
their cellulolytic potential. Int J Microbiol 2012:1–5. https://doi.org/10.1155/2012/578925
Jain M, Gupta AK, Chatterjee S (2014) To optimize the process of alcohol production from banana
peel. Recent Adv Bioenergy Res 3:208–217
Kaur M (2012) Isolation and Screening of cellulose degrading bacteria in kitchen waste and detecting
their degrading potential. IOSR J Mech Civil Eng 1(2):33–35. https://doi.org/10.9790/1684-012
3335
Klemm D, Heublein B, Fink HP et al (2005) Cellulose: fascinating biopolymer and sustainable raw
material. Angew Chem Int Edit 44:3358–3393. https://doi.org/10.1002/anie.200460587
Kumakura M (1997) Preparation of immobilized cellulase beads and their application to hydrolysis of cellulosic materials. Process Biochem 32(7):555–559. https://doi.org/10.1016/s0032-959
2(97)00011-3