Value Addition of Agricultural Wastes
for Improved Production of Industrially
Important Enzymes by Employing
Co-cultivation of Fungi
Sayari Majumdar and Jayati Bhowal
Abstract The aim of the present work was to evaluate the potential of cauliflower
wastes for the cost-effective production of industrially important enzymes using
mono and co-culture of Aspergillus niger, Aspergillus oryzae and Candida intermedia under submerged fermentation. All fungal strains were able to use cauliflower
wastes as sole carbon source for producing enzymes such as cellulases and xylanase.
Organosolvent pretreatment of cauliflower wastes relatively removed more hemicelluloses and lignin in lignocellulosic waste residues than acid and alkali treatment. Co-cultivation produced higher amount of lignocellulose degrading enzyme
system than the mono-culture used. Crude cellulases and xylanase of mono and
co-culture produced on the organosolvent pretreated cauliflower wastes with the
highest cellulose content were further tested for the release of reducing sugar during
the saccharification process of same pretreated waste biomasses. According to our
results, fungal filtrate harvested from submerged fermentation was more efficient for
lignocellulolytic biomass hydrolysis than the commercial enzymes.
Keywords Cauliflower wastes · Pretreatment · Cellulases · Xylanase ·
Filamentous fungi
1 Introduction
High production cost of pure lignocellulose degrading enzymes is the major obstacle
in the widespread commercialization of enzymatic lignocellulosic biomass hydrolysis and also contributes to our environmental pollution problem. One potential
S. Majumdar · J. Bhowal (B)
School of Community Science and Technology, Indian Institute of Engineering Science and
Technology, Shibpur, Howrah, India
e-mail: jayatibhowal@gmail.com
S. Majumdar
e-mail: sayari.majumdar@gmail.com
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_27
265
for Improved Production of Industrially
Important Enzymes by Employing
Co-cultivation of Fungi
Sayari Majumdar and Jayati Bhowal
Abstract The aim of the present work was to evaluate the potential of cauliflower
wastes for the cost-effective production of industrially important enzymes using
mono and co-culture of Aspergillus niger, Aspergillus oryzae and Candida intermedia under submerged fermentation. All fungal strains were able to use cauliflower
wastes as sole carbon source for producing enzymes such as cellulases and xylanase.
Organosolvent pretreatment of cauliflower wastes relatively removed more hemicelluloses and lignin in lignocellulosic waste residues than acid and alkali treatment. Co-cultivation produced higher amount of lignocellulose degrading enzyme
system than the mono-culture used. Crude cellulases and xylanase of mono and
co-culture produced on the organosolvent pretreated cauliflower wastes with the
highest cellulose content were further tested for the release of reducing sugar during
the saccharification process of same pretreated waste biomasses. According to our
results, fungal filtrate harvested from submerged fermentation was more efficient for
lignocellulolytic biomass hydrolysis than the commercial enzymes.
Keywords Cauliflower wastes · Pretreatment · Cellulases · Xylanase ·
Filamentous fungi
1 Introduction
High production cost of pure lignocellulose degrading enzymes is the major obstacle
in the widespread commercialization of enzymatic lignocellulosic biomass hydrolysis and also contributes to our environmental pollution problem. One potential
S. Majumdar · J. Bhowal (B)
School of Community Science and Technology, Indian Institute of Engineering Science and
Technology, Shibpur, Howrah, India
e-mail: jayatibhowal@gmail.com
S. Majumdar
e-mail: sayari.majumdar@gmail.com
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_27
265