Synthesis and Characterization of MgO
Nanoparticle and Its In Vitro Cytotoxic
Effect on Erythrocytes
Bitopan Boro, Anup Kr. Nath, Manash Barthakur, and Pankaj Kalita
Abstract Nanomaterials have moved into modern biological and medicinal implications for the advancement of biomedical applications. Magnesium oxide is a basic
oxide of interest that has many applications in catalysis, adsorption, and synthesis of
refractory ceramics as well as in the biological system. Magnesium oxide nanoparticles are characterized with a wide variety of applications and are mass-produced
throughout the world. However, questions remain regarding its safety. There has been
paucity of toxicology research on its side effects, especially under in vivo conditions.
The present paper aims at evaluating the toxicity of chemically synthesized magnesium oxide nanoparticles in erythrocytes or red blood cells (RBCs). The synthesized
MgO nanoparticles were characterized using UV-Vis spectroscopy, DLS, FTIR, and
TEM analysis. The synthesized MgO showed hemolytic activity in a dose-dependent
sequence. Size and charge characteristics of MgO observed supposed to be the influencing factor for the toxicity to the erythrocytes. Functionalization and surface modification of the synthesized MgO will help in its better applicability in biomedical
science.
Keywords Nanoparticle · MgO · Erythrocytes · Cytotoxicity
B. Boro · P. Kalita (B)
Department of Biophysics, Pub Kamrup College, Baihata Chariali, Kamrup, Assam, India
e-mail: kalita.pankaj9@gmail.com
B. Boro
e-mail: bitopanboro@rediffmail.com
A. Kr. Nath
Department of Physics, Cotton University, Guwahati, Assam, India
e-mail: anup.tu@gmail.com
M. Barthakur
Department of Zoology, Pub Kamrup College, Baihata Chariali, Kamrup, Assam, India
e-mail: researchpkc@gmail.com
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_20
199
Nanoparticle and Its In Vitro Cytotoxic
Effect on Erythrocytes
Bitopan Boro, Anup Kr. Nath, Manash Barthakur, and Pankaj Kalita
Abstract Nanomaterials have moved into modern biological and medicinal implications for the advancement of biomedical applications. Magnesium oxide is a basic
oxide of interest that has many applications in catalysis, adsorption, and synthesis of
refractory ceramics as well as in the biological system. Magnesium oxide nanoparticles are characterized with a wide variety of applications and are mass-produced
throughout the world. However, questions remain regarding its safety. There has been
paucity of toxicology research on its side effects, especially under in vivo conditions.
The present paper aims at evaluating the toxicity of chemically synthesized magnesium oxide nanoparticles in erythrocytes or red blood cells (RBCs). The synthesized
MgO nanoparticles were characterized using UV-Vis spectroscopy, DLS, FTIR, and
TEM analysis. The synthesized MgO showed hemolytic activity in a dose-dependent
sequence. Size and charge characteristics of MgO observed supposed to be the influencing factor for the toxicity to the erythrocytes. Functionalization and surface modification of the synthesized MgO will help in its better applicability in biomedical
science.
Keywords Nanoparticle · MgO · Erythrocytes · Cytotoxicity
B. Boro · P. Kalita (B)
Department of Biophysics, Pub Kamrup College, Baihata Chariali, Kamrup, Assam, India
e-mail: kalita.pankaj9@gmail.com
B. Boro
e-mail: bitopanboro@rediffmail.com
A. Kr. Nath
Department of Physics, Cotton University, Guwahati, Assam, India
e-mail: anup.tu@gmail.com
M. Barthakur
Department of Zoology, Pub Kamrup College, Baihata Chariali, Kamrup, Assam, India
e-mail: researchpkc@gmail.com
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_20
199