Isolation of Cellulose-Degrading Bacteria
and to Use Their Cellulolytic Potential
for Production of Bioethanol from Paper
Waste
Sayan Chatterjee, Kalyani Tripathi, and Ram Singh Purty
Abstract In the present scenario, solid waste management has emerged as a huge
problem for the environment and mankind. Most of the wastes are generated from
the plant source that is made up of cellulose. Cellulose can serve as the source of
glucose by various methods of degradation that can be chemical or enzymatic. The
cellulase enzyme of the bacterial system can also be used for cellulose degradation. In
the present study, cellulose-degrading bacteria have been isolated from soil obtained
from wood habitat. To indicate the cellulase activity of the organisms, the diameter of
clear zone around the colony and hydrolytic value on cellulose Congo Red agar media
were measured. The strain with maximum cellulolytic potential was selected on the
basis of DNS assay. The selected strain was further characterized at the molecular
level using 16S RNA sequencing. The characterized strain was later used for the
production of glucose by degrading the tissue paper. The glucose such produced
was used for the production of bioethanol using Saccharomyces cerevisiae. The
production of ethanol was positively tested after 6 days by iodoform test and amount
of alcohol produced was obtained by distillation of the fermented mash.
Keywords Cellulose · Cellulolytic bacteria · Paper waste · Bioconversion ·
Bioethanol
S. Chatterjee · K. Tripathi · R. S. Purty (B)
University School of Biotechnology, Guru Gobind Singh Indraprastha University, New Delhi,
India
e-mail: rspurty@ipu.ac.in
S. Chatterjee
e-mail: sayan@ipu.ac.in
K. Tripathi
e-mail: kalyanitripathi8@gmail.com
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_1
3
and to Use Their Cellulolytic Potential
for Production of Bioethanol from Paper
Waste
Sayan Chatterjee, Kalyani Tripathi, and Ram Singh Purty
Abstract In the present scenario, solid waste management has emerged as a huge
problem for the environment and mankind. Most of the wastes are generated from
the plant source that is made up of cellulose. Cellulose can serve as the source of
glucose by various methods of degradation that can be chemical or enzymatic. The
cellulase enzyme of the bacterial system can also be used for cellulose degradation. In
the present study, cellulose-degrading bacteria have been isolated from soil obtained
from wood habitat. To indicate the cellulase activity of the organisms, the diameter of
clear zone around the colony and hydrolytic value on cellulose Congo Red agar media
were measured. The strain with maximum cellulolytic potential was selected on the
basis of DNS assay. The selected strain was further characterized at the molecular
level using 16S RNA sequencing. The characterized strain was later used for the
production of glucose by degrading the tissue paper. The glucose such produced
was used for the production of bioethanol using Saccharomyces cerevisiae. The
production of ethanol was positively tested after 6 days by iodoform test and amount
of alcohol produced was obtained by distillation of the fermented mash.
Keywords Cellulose · Cellulolytic bacteria · Paper waste · Bioconversion ·
Bioethanol
S. Chatterjee · K. Tripathi · R. S. Purty (B)
University School of Biotechnology, Guru Gobind Singh Indraprastha University, New Delhi,
India
e-mail: rspurty@ipu.ac.in
S. Chatterjee
e-mail: sayan@ipu.ac.in
K. Tripathi
e-mail: kalyanitripathi8@gmail.com
© Springer Nature Singapore Pte Ltd. 2021
D. Ramkrishna et al. (eds.), Advances in Bioprocess Engineering and Technology,
Lecture Notes in Bioengineering,
https://doi.org/10.1007/978-981-15-7409-2_1
3