128
S. Ghosal et al.
Fig. 3 ABTS radical scavenging activity (%) of the sample (WUF—Whole unfermented sample,
WF—Whole fermented sample, DUF—Defatted unfermented sample, DF—Defatted fermented
sample)
3.8 Reducing Power Activity
Reducing power assay measures the electron-donating capacity of an antioxidant
(Yen and Chen 1995). The fermented flaxseed extracts had higher reducing power
activity than unfermented seed extracts. Results were shown in Table 2.
3.9 Identification of Phenolic and Flavonoid Compounds
for Defatted Unfermented and Fermented Flaxseed
Extracts by HPLC
HPLC was done for defatted unfermented and fermented extracts because they
contain higher total phenolic and flavonoid than whole unfermented and fermented
sample. The chromatographic profile of defatted unfermented and fermented flaxseed
extracts was presented in Table 3. Figure 4 and 5 showed the chromatograms of the
defatted unfermented and fermented flaxseed extracts. In this study aspartic acid,
gallic acid, valinic acid, vitamin-p, transcenamic acid, ferulic acid, elachic acid,
quercetin, kamferol were detected for both samples. Lignan which is an important
phytochemical had been identified in defatted fermented flaxseed extracts and it has
antioxidant and anticancer properties.