126
S. Ghosal et al.
Fig. 1 DPPH free radical scavenging activity (%) of the sample (WUF—Whole unfermented
sample, WF—Whole fermented sample, DUF—Defatted unfermented sample, DF—Defatted
fermented sample)
3.4 Antioxidant Activity by DPPH
DPPH radical scavenging ability of the fermented and unfermented flaxseed extracts
was presented in Figure 1. The IC 50 value represents the concentration of sample
extract required to decrease DPPH activity by 50%. The lower IC 50 value indicates
higher antioxidant activity in he sample. The defatted fermented sample showed
maximum DPPH scavenging effect of 42.33 ± 0.3% in 0.3 mg/ml concentration with
I C50 value of 0.431 ± 0.004. The DPPH radical scavenging ability (IC 50 value) of
the unfermented and fermented flaxseed extracts were showed in Table 2. It showed
that fermentation increased the scavenging ability of whole and defatted flaxseed
extracts. The DPPH radical scavenging activity of L. plantarum fermented Myrtus
communis berries higher than the unfermented berries (Curiel et al. 2015), similar to
our results.
3.5 Fe 2+ Chelating Activity
The chelating activity of the extracts is showed in Fig. 2. Chelation of transition
metal is a mechanism of antioxidative action. Chelating agents help to disrupt the
complex formation with the result that the red colour of the complex is decreased. The
chelating activity is measured by the measurement of colour reduction. The metal
chelating activity of the fermented and unfermented flaxseed extracts was presented
S. Ghosal et al.
Fig. 1 DPPH free radical scavenging activity (%) of the sample (WUF—Whole unfermented
sample, WF—Whole fermented sample, DUF—Defatted unfermented sample, DF—Defatted
fermented sample)
3.4 Antioxidant Activity by DPPH
DPPH radical scavenging ability of the fermented and unfermented flaxseed extracts
was presented in Figure 1. The IC 50 value represents the concentration of sample
extract required to decrease DPPH activity by 50%. The lower IC 50 value indicates
higher antioxidant activity in he sample. The defatted fermented sample showed
maximum DPPH scavenging effect of 42.33 ± 0.3% in 0.3 mg/ml concentration with
I C50 value of 0.431 ± 0.004. The DPPH radical scavenging ability (IC 50 value) of
the unfermented and fermented flaxseed extracts were showed in Table 2. It showed
that fermentation increased the scavenging ability of whole and defatted flaxseed
extracts. The DPPH radical scavenging activity of L. plantarum fermented Myrtus
communis berries higher than the unfermented berries (Curiel et al. 2015), similar to
our results.
3.5 Fe 2+ Chelating Activity
The chelating activity of the extracts is showed in Fig. 2. Chelation of transition
metal is a mechanism of antioxidative action. Chelating agents help to disrupt the
complex formation with the result that the red colour of the complex is decreased. The
chelating activity is measured by the measurement of colour reduction. The metal
chelating activity of the fermented and unfermented flaxseed extracts was presented