viii
Part IV β-laCtamaSe SenSorS
10 Creation of Antigen-Dependent β-Lactamase Fusion Protein Tethered
by Circularly Permuted Antibody Variable Domains . . . . . . . . . . . . . . . . . . . . 149
Hiroto Iwai, Miki Kojima-Misaizu, Jinhua Dong, and Hiroshi Ueda
11 Protein and Protease Sensing by Allosteric Derepression . . . . . . . . . . . . . . . . . 167
Hui Chin Goh, Farid J. Ghadessy, and Saurabh Nirantar
12 DNA-Specific Biosensors Based on Intramolecular β-Lactamase-Inhibitor
Complex Formation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 179
Wouter Engelen and Maarten Merkx
Part V ProteolytIC SenSorS
13 Engineering and Characterizing Synthetic Protease Sensors and Switches . . . . 197
Viktor Stein and Kirill Alexandrov
14 Characterizing Dynamic Protein–Protein Interactions Using the Genetically
Encoded Split Biosensor Assay Technique Split TEV . . . . . . . . . . . . . . . . . . . . 219
Jan P. Wintgens, Moritz J. Rossner, and Michael C. Wehr
Part VI oPtoGenetIC SwItCheS
15 Development of a Synthetic Switch to Control Protein Stability
in Eukaryotic Cells with Light. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 241
Christof Taxis
16 Light-Regulated Protein Kinases Based on the CRY2-CIB1 System. . . . . . . . . 257
Wignand W.D. Mühlhäuser, Maximilian Hörner, Wilfried Weber,
and Gerald Radziwill
17 Yeast-Based Screening System for the Selection of Functional
Light-Driven K
+
Channels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 271
Cristian Cosentino, Laura Alberio, Gerhard Thiel, and Anna Moroni
18 Primer-Aided Truncation for the Creation of Hybrid Proteins . . . . . . . . . . . . . 287
Robert Stabel, Birthe Stüven, Robert Ohlendorf, and Andreas Möglich
Part VII Cellular SIGnalInG SwItCheS
19 Engineering Small Molecule Responsive Split Protein Kinases . . . . . . . . . . . . . 307
Javier Castillo-Montoya and Indraneel Ghosh
20 Directed Evolution Methods to Rewire Signaling Networks . . . . . . . . . . . . . . 321
Raphaël B. Di Roberto, Benjamin M. Scott, and Sergio G. Peisajovich
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 339
Contents
Part IV β-laCtamaSe SenSorS
10 Creation of Antigen-Dependent β-Lactamase Fusion Protein Tethered
by Circularly Permuted Antibody Variable Domains . . . . . . . . . . . . . . . . . . . . 149
Hiroto Iwai, Miki Kojima-Misaizu, Jinhua Dong, and Hiroshi Ueda
11 Protein and Protease Sensing by Allosteric Derepression . . . . . . . . . . . . . . . . . 167
Hui Chin Goh, Farid J. Ghadessy, and Saurabh Nirantar
12 DNA-Specific Biosensors Based on Intramolecular β-Lactamase-Inhibitor
Complex Formation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 179
Wouter Engelen and Maarten Merkx
Part V ProteolytIC SenSorS
13 Engineering and Characterizing Synthetic Protease Sensors and Switches . . . . 197
Viktor Stein and Kirill Alexandrov
14 Characterizing Dynamic Protein–Protein Interactions Using the Genetically
Encoded Split Biosensor Assay Technique Split TEV . . . . . . . . . . . . . . . . . . . . 219
Jan P. Wintgens, Moritz J. Rossner, and Michael C. Wehr
Part VI oPtoGenetIC SwItCheS
15 Development of a Synthetic Switch to Control Protein Stability
in Eukaryotic Cells with Light. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 241
Christof Taxis
16 Light-Regulated Protein Kinases Based on the CRY2-CIB1 System. . . . . . . . . 257
Wignand W.D. Mühlhäuser, Maximilian Hörner, Wilfried Weber,
and Gerald Radziwill
17 Yeast-Based Screening System for the Selection of Functional
Light-Driven K
+
Channels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 271
Cristian Cosentino, Laura Alberio, Gerhard Thiel, and Anna Moroni
18 Primer-Aided Truncation for the Creation of Hybrid Proteins . . . . . . . . . . . . . 287
Robert Stabel, Birthe Stüven, Robert Ohlendorf, and Andreas Möglich
Part VII Cellular SIGnalInG SwItCheS
19 Engineering Small Molecule Responsive Split Protein Kinases . . . . . . . . . . . . . 307
Javier Castillo-Montoya and Indraneel Ghosh
20 Directed Evolution Methods to Rewire Signaling Networks . . . . . . . . . . . . . . 321
Raphaël B. Di Roberto, Benjamin M. Scott, and Sergio G. Peisajovich
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 339
Contents