55
10. Tullman J, Guntas G, Dumont M, Ostermeier
M (2011) Protein switches identified from
diverse insertion libraries created using S1 nuclease digestion of supercoiled-form plasmid DNA.
Biotechnol Bioeng 108(11):2535–2543
11. Guntas G, Mitchell SF, Ostermeier M (2004)
A molecular switch created by in vitro recombination of nonhomologous genes. Chem Biol
11(11):1483–1487
12. Guntas G, Ostermeier M (2004) Creation of
an allosteric enzyme by domain insertion.
J Mol Biol 336(1):263–273
13. Ostermeier M, Guntas G (2003) Engineering a
protein molecular switch by combinatorial
domain insertion. Abstr Pap Am Chem S
225:U243–U243
14. Guntas G, Kanwar M, Ostermeier M (2012)
Circular permutation in the omega-loop of
TEM-1 beta-lactamase results in improved
activity and altered substrate specificity. PLoS
One 7(4):e35998
15. Nicholes N, Date A, Beaujean P, Hauk P,
Kanwar M, Ostermeier M (2016) Modular
protein switches derived from antibody
mimetic proteins. Protein Eng Des Sel
29(2):77–85
16. Choi JH, Laurent AH, Hilser VJ, Ostermeier
M (2015) Design of protein switches based on
an ensemble model of allostery. Nat Commun
6: 6968
17. Choi JH, Zayats M, Searson PC, Ostermeier M
(2016) Electrochemical activation of engineered protein switches. Biotechnol Bioeng
113(2):453–456
18. Heins RA, Choi JH, Sohka T, Ostermeier M
(2011) In vitro recombination of nonhomologous genes can result in gene fusions
that confer a switching phenotype to cells.
PLoS One 6(11):e27302
19. Yu Y, Lutz S (2011) Circular permutation: a
different way to engineer enzyme structure and
function. Trends Biotechnol 29(1):18–25
20. Bosley AD, Ostermeier M (2005) Mathematical
expressions useful in the construction,
description and evaluation of protein libraries.
Biomol Eng 22(1–3):57–61
Engineering Protein Switches by Domain Insertion
10. Tullman J, Guntas G, Dumont M, Ostermeier
M (2011) Protein switches identified from
diverse insertion libraries created using S1 nuclease digestion of supercoiled-form plasmid DNA.
Biotechnol Bioeng 108(11):2535–2543
11. Guntas G, Mitchell SF, Ostermeier M (2004)
A molecular switch created by in vitro recombination of nonhomologous genes. Chem Biol
11(11):1483–1487
12. Guntas G, Ostermeier M (2004) Creation of
an allosteric enzyme by domain insertion.
J Mol Biol 336(1):263–273
13. Ostermeier M, Guntas G (2003) Engineering a
protein molecular switch by combinatorial
domain insertion. Abstr Pap Am Chem S
225:U243–U243
14. Guntas G, Kanwar M, Ostermeier M (2012)
Circular permutation in the omega-loop of
TEM-1 beta-lactamase results in improved
activity and altered substrate specificity. PLoS
One 7(4):e35998
15. Nicholes N, Date A, Beaujean P, Hauk P,
Kanwar M, Ostermeier M (2016) Modular
protein switches derived from antibody
mimetic proteins. Protein Eng Des Sel
29(2):77–85
16. Choi JH, Laurent AH, Hilser VJ, Ostermeier
M (2015) Design of protein switches based on
an ensemble model of allostery. Nat Commun
6: 6968
17. Choi JH, Zayats M, Searson PC, Ostermeier M
(2016) Electrochemical activation of engineered protein switches. Biotechnol Bioeng
113(2):453–456
18. Heins RA, Choi JH, Sohka T, Ostermeier M
(2011) In vitro recombination of nonhomologous genes can result in gene fusions
that confer a switching phenotype to cells.
PLoS One 6(11):e27302
19. Yu Y, Lutz S (2011) Circular permutation: a
different way to engineer enzyme structure and
function. Trends Biotechnol 29(1):18–25
20. Bosley AD, Ostermeier M (2005) Mathematical
expressions useful in the construction,
description and evaluation of protein libraries.
Biomol Eng 22(1–3):57–61
Engineering Protein Switches by Domain Insertion
