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Synthetic Protein SwitcheS: MethodS and ProtocolS 341
Index
Protein stability ��241–254
Protein thermostability �� 72, 73,
75, 79
R
Radioactivity-based kinase activity assays ��310, 312–313
Reaction kinetics
chemical ��63
enzymatic��123,
204, 216
Rewiring signal transduction networks ��321–336
S
Saccharomyces cerevisiae
chemical transformation ��249–250
drop test ��283
functional complementation �� 273, 281
genetic complementation ��18
replica plating ��283
Self-labelling proteins
CLIP-tag �� 103, 110, 113
SNAP-tag �� 102, 103, 105, 108, 110, 113
Semi-synthetic sensors
bioluminescent ��101
fluorescent �� 89, 101
LUCIDs ��101–115
SNIFITs ��101–115
Solute binding proteins (SBP) �� 72, 73, 75, 76
79–82, 84, 85, 92, 93
Spectrophometry
absorbance �� 113, 331, 332
circular dichroism ��33
fluorescence ��33
luminescence �� 101, 119, 131
UV/Vis �� 62, 182, 187
Split-proteases ��219
Split protein kinase ��307–317
Split-protein sensor ��310
Split-tyrosine kinase �� 310, 316
Statistical sequence analysis ��5, 8–9
Synthetic biology �� 5, 179, 198
Synthetic dye labelling �� 90, 91, 98
Synthetic protein switches �� 3, 179, 191, 201
Synthetic signal transduction ��4
T
Therapeutic drug monitoring ��101
V
Viral potassium channel engineering ��271