Fig. 2 Fluorescence-activated cell sorting of yeast mutant libraries. In this example, we show sorting data for
yeast carrying a Ste2 receptor library, with mutants conferring a mating response to K. lactis pheromone. (a)
Gating strategy needed to ensure that most events collected correspond to single cells. The rectangular gates
capture cells in the diagonal, which mostly correspond to single cells (cell aggregates display higher forward