304
1. Pawson T, Nash P (2003) Assembly of cell regulatory systems through protein interaction
domains. Science 300:445–452
2. Gokhale RS, Khosla C (2000) Role of linkers in
communication between protein modules.
Curr Opin Chem Biol 4:22–27
3. Amet N, Lee H-F, Shen W-C (2009) Insertion
of the designed helical linker led to increased
expression of Tf-based fusion proteins. Pharm
Res 26:523–528
4. Möglich A, Yang X, Ayers RA, Moffat K (2010)
Structure and function of plant photoreceptors. Annu Rev Plant Biol 61:21–47
5. Ziegler T, Möglich A (2015) Photoreceptor
engineering. Front Mol Biosci 2:30
6. Ryu M-H, Gomelsky M (2014) Near-infrared
light responsive synthetic c-di-GMP module
for optogenetic applications. ACS Synth Biol
3:802–810
7. Wu YI, Frey D, Lungu OI, Jaehrig A,
Schlichting I, Kuhlman B, Hahn KM (2009) A
genetically encoded photoactivatable Rac controls the motility of living cells. Nature 461:
104–108
8. Möglich A, Ayers RA, Moffat K (2009) Design
and signaling mechanism of light-regulated histidine kinases. J Mol Biol 385:1433–1444
9. Diensthuber RP, Bommer M, Gleichmann T,
Möglich A (2013) Full-length structure of a
sensor histidine kinase pinpoints coaxial coiled
coils as signal transducers and modulators.
Structure 21:1127–1136
10. Ohlendorf R, Schumacher CH, Richter F,
Möglich A (2016) Library-aided probing of
linker determinants in hybrid photoreceptors.
ACS Synth Biol 5(10):1117–26. doi:10.1021/
acssynbio.6b00028.
11. Ostermeier M, Shim JH, Benkovic SJ (1999) A
combinatorial approach to hybrid enzymes
independent of DNA homology. Nat
Biotechnol 17:1205–1209
12. Ostermeier M, Nixon AE, Shim JH, Benkovic
SJ (1999) Combinatorial protein engineering
by incremental truncation. Proc Natl Acad Sci
96:3562–3567
13. Lutz S, Ostermeier M (2003). Preparation of
SCRATCHY hybrid protein libraries. Directed
Evolution Library Creation: Methods and
Protocols 143–151
14. Arnold FH, Georgiou G (2003) Methods in
molecular biology, vol. 231. Directed evolution
library creation methods and protocols.
Humana Press, Totowa, NJ
15. Ostermeier M (2003) Theoretical distribution
of truncation lengths in incremental truncation
libraries. Biotechnol Bioeng 82:564–577
16. Ohlendorf R, Vidavski RR, Eldar A, Moffat K,
Möglich A (2012) From dusk till dawn: oneplasmid systems for light-regulated gene
expression. J Mol Biol 416:534–542
17. Gleichmann T, Diensthuber RP, Möglich A
(2013) Charting the signal trajectory in a lightoxygen- voltage photoreceptor by random
mutagenesis and covariance analysis. J Biol
Chem 288:29345–29355
18. Lupas AN, Gruber M (2005) The structure of
alpha-helical coiled coils. Adv Protein Chem
70:37–78
19. Engelhard C, Diensthuber RP, Möglich A, Bittl
R (2016) Blue-light reception through quaternary transitions. Submitted
20. Berntsson O, Diensthuber RP, Panman MR, et
al (2016) Conformational photoactivation of a
light-oxygen-voltage photosensor. Submitted
21. Leung DW, Chen E, Goeddel DV (1989) A
method for random mutagenesis of a defined
DNA segment using a modified polymerase
chain reaction. Technique 1:11–15
References
Robert Stabel et al.
1. Pawson T, Nash P (2003) Assembly of cell regulatory systems through protein interaction
domains. Science 300:445–452
2. Gokhale RS, Khosla C (2000) Role of linkers in
communication between protein modules.
Curr Opin Chem Biol 4:22–27
3. Amet N, Lee H-F, Shen W-C (2009) Insertion
of the designed helical linker led to increased
expression of Tf-based fusion proteins. Pharm
Res 26:523–528
4. Möglich A, Yang X, Ayers RA, Moffat K (2010)
Structure and function of plant photoreceptors. Annu Rev Plant Biol 61:21–47
5. Ziegler T, Möglich A (2015) Photoreceptor
engineering. Front Mol Biosci 2:30
6. Ryu M-H, Gomelsky M (2014) Near-infrared
light responsive synthetic c-di-GMP module
for optogenetic applications. ACS Synth Biol
3:802–810
7. Wu YI, Frey D, Lungu OI, Jaehrig A,
Schlichting I, Kuhlman B, Hahn KM (2009) A
genetically encoded photoactivatable Rac controls the motility of living cells. Nature 461:
104–108
8. Möglich A, Ayers RA, Moffat K (2009) Design
and signaling mechanism of light-regulated histidine kinases. J Mol Biol 385:1433–1444
9. Diensthuber RP, Bommer M, Gleichmann T,
Möglich A (2013) Full-length structure of a
sensor histidine kinase pinpoints coaxial coiled
coils as signal transducers and modulators.
Structure 21:1127–1136
10. Ohlendorf R, Schumacher CH, Richter F,
Möglich A (2016) Library-aided probing of
linker determinants in hybrid photoreceptors.
ACS Synth Biol 5(10):1117–26. doi:10.1021/
acssynbio.6b00028.
11. Ostermeier M, Shim JH, Benkovic SJ (1999) A
combinatorial approach to hybrid enzymes
independent of DNA homology. Nat
Biotechnol 17:1205–1209
12. Ostermeier M, Nixon AE, Shim JH, Benkovic
SJ (1999) Combinatorial protein engineering
by incremental truncation. Proc Natl Acad Sci
96:3562–3567
13. Lutz S, Ostermeier M (2003). Preparation of
SCRATCHY hybrid protein libraries. Directed
Evolution Library Creation: Methods and
Protocols 143–151
14. Arnold FH, Georgiou G (2003) Methods in
molecular biology, vol. 231. Directed evolution
library creation methods and protocols.
Humana Press, Totowa, NJ
15. Ostermeier M (2003) Theoretical distribution
of truncation lengths in incremental truncation
libraries. Biotechnol Bioeng 82:564–577
16. Ohlendorf R, Vidavski RR, Eldar A, Moffat K,
Möglich A (2012) From dusk till dawn: oneplasmid systems for light-regulated gene
expression. J Mol Biol 416:534–542
17. Gleichmann T, Diensthuber RP, Möglich A
(2013) Charting the signal trajectory in a lightoxygen- voltage photoreceptor by random
mutagenesis and covariance analysis. J Biol
Chem 288:29345–29355
18. Lupas AN, Gruber M (2005) The structure of
alpha-helical coiled coils. Adv Protein Chem
70:37–78
19. Engelhard C, Diensthuber RP, Möglich A, Bittl
R (2016) Blue-light reception through quaternary transitions. Submitted
20. Berntsson O, Diensthuber RP, Panman MR, et
al (2016) Conformational photoactivation of a
light-oxygen-voltage photosensor. Submitted
21. Leung DW, Chen E, Goeddel DV (1989) A
method for random mutagenesis of a defined
DNA segment using a modified polymerase
chain reaction. Technique 1:11–15
References
Robert Stabel et al.