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The screening is performed in a temperature-controlled chamber
where culture plates can be either exposed to homogeneous light
irradiation or kept in the dark. Figure 2 shows a schematic drawing
of the chamber, from the side view (Fig. 2a) and from the top
(Fig. 2b).
1. Homogenous light irradiation is provided as follows: a metal
scaffold is built around a 1 m
2
surface, in order to place 9
LED assemblies 70 cm above cellular cultures (see Note 6).
Each LED assembly is composed of a heat sink mounted on
the top of a Tri-star LED, through thermal conductive adhesive tape. The thermal sink allows an adequate passive cooling
system that ensures long-time LED functionality (Fig. 2a).
LED assemblies are mounted on three metal bars to allow
2.8 Light/Dark
Differential Screening
Chamber
Fig. 2 Growth chamber for yeast-based light/dark complementation assay: (a) side view of the incubation
chamber; (b) top view of the workbench. Detailed scheme of LED assembly and LED components is shown at
the right side of each panel
Cristian Cosentino et al.