238
in vitro sensors of protein protein interactions.
Nat Biotechnol 20:619–622. doi:10.1038/
nbt0602-619
29. Blakely BT, Rossi FM, Tillotson B, Palmer M,
Estelles A, Blau HM (2000) Epidermal growth
factor receptor dimerization monitored in live
cells. Nat Biotechnol 18:218–222. doi:10.1038/
72686
30. Rossi F, Charlton CA, Blau HM (1997)
Monitoring protein-protein interactions in
intact eukaryotic cells by beta-galactosidase
complementation. Proc Natl Acad Sci U S A
94:8405–8410
31. Johnsson N, Varshavsky A (1994) Split ubiquitin as a sensor of protein interactions in vivo.
Proc Natl Acad Sci U S A 91:10340–10344
32. Stagljar I, Korostensky C, Johnsson N, Heesen
S et al (1998) A genetic system based on splitubiquitin for the analysis of interactions
between membrane proteins in vivo. PNAS
95:5187–5192
33. Wehr MC, Laage R, Bolz U, Fischer TM,
Grünewald S, Scheek S, Bach A, Nave K-A,
Rossner MJ (2006) Monitoring regulated
protein- protein interactions using split TEV. Nat
Methods 3:985–993. doi:10.1038/nmeth967
34. Hamdan FF, Audet M, Garneau P, Pelletier J,
Bouvier M (2005) High-throughput screening
of G protein-coupled receptor antagonists using
a bioluminescence resonance energy transfer
1-based beta-arrestin2 recruitment assay.
J Biomol Screen 10:463–475. doi:10.1177/
1087057105275344
35. Vrecl M, Jorgensen R, Pogacnik A, Heding A
(2004) Development of a BRET2 screening assay
using beta-arrestin 2 mutants. J Biomol Screen
9:322–333. doi:10.1177/1087057104263212
36. Kapust RB, Tözsér J, Fox JD, Anderson DE,
Cherry S, Copeland TD, Waugh DS (2001)
Tobacco etch virus protease: mechanism of
autolysis and rational design of stable mutants
with wild-type catalytic proficiency. Protein
Eng 14:993–1000
37. Nunn CM, Jeeves M, Cliff MJ, Urquhart GT,
George RR, Chao LH, Tscuchia Y, Djordjevic
S (2005) Crystal structure of tobacco etch virus
protease shows the protein C terminus bound
within the active site. J Mol Biol 350:145–155.
doi:10.1016/j.jmb.2005.04.013
38. Wehr MC, Galinski S, Rossner MJ (2015)
Monitoring G Protein-Coupled Receptor
Activation Using the Protein Fragment
Complementation Technique Split TEV.
Methods
Mol
Biol
1272:107–118.
doi:10.1007/978-1-4939-2336-6_8
39. Praskova M, Khoklatchev A, Ortiz-Vega S,
Avruch J (2004) Regulation of the MST1
kinase by autophosphorylation, by the growth
inhibitory proteins, RASSF1 and NORE1, and
by Ras. The Biochemical journal 381:453–462
40. Genevet A, Wehr MC, Brain R, Thompson BJ,
Tapon N (2010) Kibra is a regulator of the
Salvador/Warts/Hippo signaling network.
Dev Cell 18:300–308. doi:10.1016/j.devcel.
2009.12.011
41. Wehr MC, Holder MV, Gailite I, Saunders RE,
Maile TM, Ciirdaeva E, Instrell R, Jiang M,
Howell M, Rossner MJ, Tapon N (2013) Saltinducible kinases regulate growth through the
Hippo signalling pathway in Drosophila. Nat
Cell Biol 15:61–71. doi:10.1038/ncb2658
42. Botvinnik A, Wichert SP, Fischer TM, Rossner
MJ (2010) Integrated analysis of receptor activation and downstream signaling with
EXTassays. Nat Meth 7:74–80. doi:10.1038/
nmeth.1407
43. Ura S, Masuyama N, Graves JD, Gotoh Y
(2001) Caspase cleavage of MST1 promotes
nuclear translocation and chromatin condensation. Proc Natl Acad Sci USA 98:10148–
10153. doi:10.1073/pnas.181161698
44. Stark C, Breitkreutz B-J, Reguly T, Boucher L,
Breitkreutz A, Tyers M (2006) BioGRID: a
general repository for interaction datasets.
Nucleic Acids Res 34:D535–539. doi:10.1093/
nar/gkj109
Jan P. Wintgens et al.
in vitro sensors of protein protein interactions.
Nat Biotechnol 20:619–622. doi:10.1038/
nbt0602-619
29. Blakely BT, Rossi FM, Tillotson B, Palmer M,
Estelles A, Blau HM (2000) Epidermal growth
factor receptor dimerization monitored in live
cells. Nat Biotechnol 18:218–222. doi:10.1038/
72686
30. Rossi F, Charlton CA, Blau HM (1997)
Monitoring protein-protein interactions in
intact eukaryotic cells by beta-galactosidase
complementation. Proc Natl Acad Sci U S A
94:8405–8410
31. Johnsson N, Varshavsky A (1994) Split ubiquitin as a sensor of protein interactions in vivo.
Proc Natl Acad Sci U S A 91:10340–10344
32. Stagljar I, Korostensky C, Johnsson N, Heesen
S et al (1998) A genetic system based on splitubiquitin for the analysis of interactions
between membrane proteins in vivo. PNAS
95:5187–5192
33. Wehr MC, Laage R, Bolz U, Fischer TM,
Grünewald S, Scheek S, Bach A, Nave K-A,
Rossner MJ (2006) Monitoring regulated
protein- protein interactions using split TEV. Nat
Methods 3:985–993. doi:10.1038/nmeth967
34. Hamdan FF, Audet M, Garneau P, Pelletier J,
Bouvier M (2005) High-throughput screening
of G protein-coupled receptor antagonists using
a bioluminescence resonance energy transfer
1-based beta-arrestin2 recruitment assay.
J Biomol Screen 10:463–475. doi:10.1177/
1087057105275344
35. Vrecl M, Jorgensen R, Pogacnik A, Heding A
(2004) Development of a BRET2 screening assay
using beta-arrestin 2 mutants. J Biomol Screen
9:322–333. doi:10.1177/1087057104263212
36. Kapust RB, Tözsér J, Fox JD, Anderson DE,
Cherry S, Copeland TD, Waugh DS (2001)
Tobacco etch virus protease: mechanism of
autolysis and rational design of stable mutants
with wild-type catalytic proficiency. Protein
Eng 14:993–1000
37. Nunn CM, Jeeves M, Cliff MJ, Urquhart GT,
George RR, Chao LH, Tscuchia Y, Djordjevic
S (2005) Crystal structure of tobacco etch virus
protease shows the protein C terminus bound
within the active site. J Mol Biol 350:145–155.
doi:10.1016/j.jmb.2005.04.013
38. Wehr MC, Galinski S, Rossner MJ (2015)
Monitoring G Protein-Coupled Receptor
Activation Using the Protein Fragment
Complementation Technique Split TEV.
Methods
Mol
Biol
1272:107–118.
doi:10.1007/978-1-4939-2336-6_8
39. Praskova M, Khoklatchev A, Ortiz-Vega S,
Avruch J (2004) Regulation of the MST1
kinase by autophosphorylation, by the growth
inhibitory proteins, RASSF1 and NORE1, and
by Ras. The Biochemical journal 381:453–462
40. Genevet A, Wehr MC, Brain R, Thompson BJ,
Tapon N (2010) Kibra is a regulator of the
Salvador/Warts/Hippo signaling network.
Dev Cell 18:300–308. doi:10.1016/j.devcel.
2009.12.011
41. Wehr MC, Holder MV, Gailite I, Saunders RE,
Maile TM, Ciirdaeva E, Instrell R, Jiang M,
Howell M, Rossner MJ, Tapon N (2013) Saltinducible kinases regulate growth through the
Hippo signalling pathway in Drosophila. Nat
Cell Biol 15:61–71. doi:10.1038/ncb2658
42. Botvinnik A, Wichert SP, Fischer TM, Rossner
MJ (2010) Integrated analysis of receptor activation and downstream signaling with
EXTassays. Nat Meth 7:74–80. doi:10.1038/
nmeth.1407
43. Ura S, Masuyama N, Graves JD, Gotoh Y
(2001) Caspase cleavage of MST1 promotes
nuclear translocation and chromatin condensation. Proc Natl Acad Sci USA 98:10148–
10153. doi:10.1073/pnas.181161698
44. Stark C, Breitkreutz B-J, Reguly T, Boucher L,
Breitkreutz A, Tyers M (2006) BioGRID: a
general repository for interaction datasets.
Nucleic Acids Res 34:D535–539. doi:10.1093/
nar/gkj109
Jan P. Wintgens et al.