218
9. Li S, Xu L, Hua H et al (2007) A set of
UV-inducible autolytic vectors for high
throughput screening. J Biotechnol 127:647–
652. doi:10.1016/j.jbiotec.2006.07.030
10. Studier FW (2005) Protein production by
auto-induction in high-density shaking cultures. Protein Expr Purif 41:207–234.
doi:10.1016/j.pep.2005.01.016
11. Kapust RB, Tözsér J, Fox JD et al (2001)
Tobacco etch virus protease: mechanism of
autolysis and rational design of stable mutants
with wild-type catalytic proficiency. Protein
Eng 14:993–1000. doi:10.1093/protein/
14.12.993
12. Kapust RB, Waugh DS (1999) Escherichia coli
maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides
to which it is fused. Protein Sci 8:1668–1674.
doi:10.1110/ps.8.8.1668
13. Raran-Kurussi S, Waugh DS (2012) The ability
to enhance the solubility of its fusion partners
is an intrinsic property of maltose-binding protein but their folding is either spontaneous or
chaperone-mediated. PLoS One 7:e49589.
doi:10.1371/journal.pone.0049589
14. Stein V, Hollfelder F (2009) An efficient
method to assemble linear DNA templates for
in vitro screening and selection systems.
Nucleic Acids Res. doi:10.1093/nar/gkp589
15. Kapust RB, Tozser J, Copeland TD, Waugh
DS (2002) The P1’ specificity of tobacco etch
virus protease. Biochem Biophys Res Commun
294:949–955. doi:10.1016/S0006-291X(02)
00574-0
16. Posern G, Zheng J, Knudsen BS et al (1998)
Development of highly selective SH3 binding
peptides for Crk and CRKL which disrupt Crkcomplexes with DOCK180, SoS and
C3G. Oncogene 16:1903–1912. doi:10.1038/
sj.onc.1201714
17. Cai Z, Xu W, Xue R, Lin Z (2008) Facile,
reagentless and in situ release of Escherichia coli
intracellular enzymes by heat-inducible autolytic vector for high-throughput screening.
Protein
Eng
Des
Sel
21:681–687.
doi:10.1093/protein/gzn049
Viktor Stein and Kirill Alexandrov
9. Li S, Xu L, Hua H et al (2007) A set of
UV-inducible autolytic vectors for high
throughput screening. J Biotechnol 127:647–
652. doi:10.1016/j.jbiotec.2006.07.030
10. Studier FW (2005) Protein production by
auto-induction in high-density shaking cultures. Protein Expr Purif 41:207–234.
doi:10.1016/j.pep.2005.01.016
11. Kapust RB, Tözsér J, Fox JD et al (2001)
Tobacco etch virus protease: mechanism of
autolysis and rational design of stable mutants
with wild-type catalytic proficiency. Protein
Eng 14:993–1000. doi:10.1093/protein/
14.12.993
12. Kapust RB, Waugh DS (1999) Escherichia coli
maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides
to which it is fused. Protein Sci 8:1668–1674.
doi:10.1110/ps.8.8.1668
13. Raran-Kurussi S, Waugh DS (2012) The ability
to enhance the solubility of its fusion partners
is an intrinsic property of maltose-binding protein but their folding is either spontaneous or
chaperone-mediated. PLoS One 7:e49589.
doi:10.1371/journal.pone.0049589
14. Stein V, Hollfelder F (2009) An efficient
method to assemble linear DNA templates for
in vitro screening and selection systems.
Nucleic Acids Res. doi:10.1093/nar/gkp589
15. Kapust RB, Tozser J, Copeland TD, Waugh
DS (2002) The P1’ specificity of tobacco etch
virus protease. Biochem Biophys Res Commun
294:949–955. doi:10.1016/S0006-291X(02)
00574-0
16. Posern G, Zheng J, Knudsen BS et al (1998)
Development of highly selective SH3 binding
peptides for Crk and CRKL which disrupt Crkcomplexes with DOCK180, SoS and
C3G. Oncogene 16:1903–1912. doi:10.1038/
sj.onc.1201714
17. Cai Z, Xu W, Xue R, Lin Z (2008) Facile,
reagentless and in situ release of Escherichia coli
intracellular enzymes by heat-inducible autolytic vector for high-throughput screening.
Protein
Eng
Des
Sel
21:681–687.
doi:10.1093/protein/gzn049
Viktor Stein and Kirill Alexandrov