164
1. Stains CI, Furman JL, Porter JR, Rajagopal S,
Li Y, Wyatt RT, Ghosh I (2010) A general
approach for receptor and antibody-targeted
detection of native proteins utilizing splitluciferase reassembly. ACS Chem Biol
5:943–952
2. Alderson RF, Toki BE, Roberge M, Geng W,
Basler J, Chin R, Liu A, Ueda R, Hodges D,
Escandon E, Chen T, Kanavarioti T, Babé L,
Senter PD, Fox JA, Schellenberger V (2006)
Characterization of a CC49-based single-chain
fragment-β-lactamase fusion protein for
antibody- directed enzyme prodrug therapy
(ADEPT). Bioconjug Chem 17:410–418
3. Schumacher FF, Sanchania VA, Tolner B,
Wright ZVF, Ryan CP, Smith MEB, Ward JM,
Caddick S, Kay CWM, Aeppli G, Chester KA,
Baker JR (2013) Homogeneous antibody fragment conjugation by disulfide bridging introduces ‘spinostics’. Sci Rep 3:1525
4. Yokozeki T, Ueda H, Arai R, Mahoney W,
Nagamune T (2002) A homogeneous noncompetitive immunoassay for the detection of
small haptens. Anal Chem 74:2500–2504
5. Ueda H, Yokozeki T, Arai R, Tsumoto K,
Kumagai I, Nagamune T (2003) An optimized
homogeneous noncompetitive immunoassay
based on the antigen-driven enzymatic complementation.
J
Immunol
Methods
279(1–2):209–218
6. Kojima M, Iwai H, Dong J, Lim S-L, Ito S,
Okumura K, Ihara M, Ueda H (2011)
Activation of circularly permutated β-lactamase
tethered to antibody domains by specific small
molecules. Bioconjug Chem 22:633–641
7. Guntas G, Mitchell SF, Ostermeier M (2004)
A Molecular switch created by in vitro
recombination of nonhomologous genes.
Chem Biol 11:1483–1487
8. Guntas G, Mansell TJ, Kim JR, Ostermeier M
(2005) Directed evolution of protein switches
and their application to the creation of ligandbinding proteins. Proc Natl Acad Sci U S A
102:11224–11229
9. Nicholes N, Date A, Beaujean P, Hauk P,
Kanwar M, Ostermeier M (2016) Modular
protein switches derived from antibody
mimetic proteins. Protein Eng Des Sel
29:77–85
10. Iwai H, Kojima-Misaizu M, Dong J, Ueda H
(2016) Creation of a ligand-dependent
enzyme by fusing circularly permuted antibody variable region domains. Bioconjug
Chem 27:868–873
11. Brinkmann U, di Carlo A, Vasmatzis G,
Kurochkina N, Beers R, Lee B, Pastan I (1997)
Stabilization of a recombinant Fv fragment by
base-loop interconnection and V(H)-V(L) permutation. J Mol Biol 268:107–117
12. Motlagh HN, Wrabl JO, Li J, Hilser VJ (2014)
The ensemble nature of allostery. Nature
508:331–339
13. Choi JH, San A, Ostermeier M (2013) Nonallosteric enzyme switches possess larger
effector- induced changes in thermodynamic
stability than their non-switch analogs. Protein
Sci 22:475–485
14. Choi JH, Ostermeier M (2015) Rational
design of a fusion protein to exhibit disulfidemediated logic gate behavior. ACS Synth Biol
4:400–406
15. Choi JH, Laurent AH, Hilser VJ, Ostermeier
M (2015) Design of protein switches based on
an ensemble model of allostery. Nat Commun
6:6968
16. Ohmuro-Matsuyama Y, Chung CI, Ueda H
(2013) Demonstration of protein-fragment
complementation assay using purified firefly
luciferase fragments. BMC Biotechnol 13:31
17. Ohiro Y, Ueda H, Shibata N, Nagamune T
(2007) Enhanced fluorescence resonance
energy transfer immunoassay with improved
sensitivity based on the Fab’-based immunoconjugates. Anal Biochem 360:266–272
18. Chung CI, Makino R, Dong J, Ueda H (2015)
Open flower fluoroimmunoassay: a general
method to make fluorescent protein-based
immunosensor probes. Anal Chem 87:
3513–3519
19. Ohmuro-Matsuyama Y, Hara Y, Ueda H
(2014) Improved protein-protein interaction
assay FlimPIA by the entrapment of luciferase
conformation. Anal Chem 86:2013–2018
20. Kurihara M, Ohmuro-Matsuyama Y, Ayabe K,
Yamashita T, Yamaji H, Ueda H (2016) Ultra
sensitive firefly luciferase-based protein-protein
interaction assay (FlimPIA) attained by hinge
region engineering and optimized reaction
conditions. Biotechnol J 11:91–99
21. de Wildt RM, Mundy CR, Gorick BD,
Tomlinson IM (2000) Antibody arrays for
high-throughput screening of antibodyantigen interactions. Nat Biotechnol 18:
989–994
22. Lim S-L, Ichinose H, Shinoda T, Ueda H
(2007) Noncompetitive detection of low
molecular weight peptides by open sandwich
immunoassay. Anal Chem 79:6193–6200
23. Ke W, Laurent AH, Armstrong MD, Chen Y,
Smith WE, Liang J, Wright CM, Ostermeier
M, van den Akker F (2012) Structure of an
engineered β-lactamase maltose binding protein
References
Hiroto Iwai et al.
1. Stains CI, Furman JL, Porter JR, Rajagopal S,
Li Y, Wyatt RT, Ghosh I (2010) A general
approach for receptor and antibody-targeted
detection of native proteins utilizing splitluciferase reassembly. ACS Chem Biol
5:943–952
2. Alderson RF, Toki BE, Roberge M, Geng W,
Basler J, Chin R, Liu A, Ueda R, Hodges D,
Escandon E, Chen T, Kanavarioti T, Babé L,
Senter PD, Fox JA, Schellenberger V (2006)
Characterization of a CC49-based single-chain
fragment-β-lactamase fusion protein for
antibody- directed enzyme prodrug therapy
(ADEPT). Bioconjug Chem 17:410–418
3. Schumacher FF, Sanchania VA, Tolner B,
Wright ZVF, Ryan CP, Smith MEB, Ward JM,
Caddick S, Kay CWM, Aeppli G, Chester KA,
Baker JR (2013) Homogeneous antibody fragment conjugation by disulfide bridging introduces ‘spinostics’. Sci Rep 3:1525
4. Yokozeki T, Ueda H, Arai R, Mahoney W,
Nagamune T (2002) A homogeneous noncompetitive immunoassay for the detection of
small haptens. Anal Chem 74:2500–2504
5. Ueda H, Yokozeki T, Arai R, Tsumoto K,
Kumagai I, Nagamune T (2003) An optimized
homogeneous noncompetitive immunoassay
based on the antigen-driven enzymatic complementation.
J
Immunol
Methods
279(1–2):209–218
6. Kojima M, Iwai H, Dong J, Lim S-L, Ito S,
Okumura K, Ihara M, Ueda H (2011)
Activation of circularly permutated β-lactamase
tethered to antibody domains by specific small
molecules. Bioconjug Chem 22:633–641
7. Guntas G, Mitchell SF, Ostermeier M (2004)
A Molecular switch created by in vitro
recombination of nonhomologous genes.
Chem Biol 11:1483–1487
8. Guntas G, Mansell TJ, Kim JR, Ostermeier M
(2005) Directed evolution of protein switches
and their application to the creation of ligandbinding proteins. Proc Natl Acad Sci U S A
102:11224–11229
9. Nicholes N, Date A, Beaujean P, Hauk P,
Kanwar M, Ostermeier M (2016) Modular
protein switches derived from antibody
mimetic proteins. Protein Eng Des Sel
29:77–85
10. Iwai H, Kojima-Misaizu M, Dong J, Ueda H
(2016) Creation of a ligand-dependent
enzyme by fusing circularly permuted antibody variable region domains. Bioconjug
Chem 27:868–873
11. Brinkmann U, di Carlo A, Vasmatzis G,
Kurochkina N, Beers R, Lee B, Pastan I (1997)
Stabilization of a recombinant Fv fragment by
base-loop interconnection and V(H)-V(L) permutation. J Mol Biol 268:107–117
12. Motlagh HN, Wrabl JO, Li J, Hilser VJ (2014)
The ensemble nature of allostery. Nature
508:331–339
13. Choi JH, San A, Ostermeier M (2013) Nonallosteric enzyme switches possess larger
effector- induced changes in thermodynamic
stability than their non-switch analogs. Protein
Sci 22:475–485
14. Choi JH, Ostermeier M (2015) Rational
design of a fusion protein to exhibit disulfidemediated logic gate behavior. ACS Synth Biol
4:400–406
15. Choi JH, Laurent AH, Hilser VJ, Ostermeier
M (2015) Design of protein switches based on
an ensemble model of allostery. Nat Commun
6:6968
16. Ohmuro-Matsuyama Y, Chung CI, Ueda H
(2013) Demonstration of protein-fragment
complementation assay using purified firefly
luciferase fragments. BMC Biotechnol 13:31
17. Ohiro Y, Ueda H, Shibata N, Nagamune T
(2007) Enhanced fluorescence resonance
energy transfer immunoassay with improved
sensitivity based on the Fab’-based immunoconjugates. Anal Biochem 360:266–272
18. Chung CI, Makino R, Dong J, Ueda H (2015)
Open flower fluoroimmunoassay: a general
method to make fluorescent protein-based
immunosensor probes. Anal Chem 87:
3513–3519
19. Ohmuro-Matsuyama Y, Hara Y, Ueda H
(2014) Improved protein-protein interaction
assay FlimPIA by the entrapment of luciferase
conformation. Anal Chem 86:2013–2018
20. Kurihara M, Ohmuro-Matsuyama Y, Ayabe K,
Yamashita T, Yamaji H, Ueda H (2016) Ultra
sensitive firefly luciferase-based protein-protein
interaction assay (FlimPIA) attained by hinge
region engineering and optimized reaction
conditions. Biotechnol J 11:91–99
21. de Wildt RM, Mundy CR, Gorick BD,
Tomlinson IM (2000) Antibody arrays for
high-throughput screening of antibodyantigen interactions. Nat Biotechnol 18:
989–994
22. Lim S-L, Ichinose H, Shinoda T, Ueda H
(2007) Noncompetitive detection of low
molecular weight peptides by open sandwich
immunoassay. Anal Chem 79:6193–6200
23. Ke W, Laurent AH, Armstrong MD, Chen Y,
Smith WE, Liang J, Wright CM, Ostermeier
M, van den Akker F (2012) Structure of an
engineered β-lactamase maltose binding protein
References
Hiroto Iwai et al.