130
1. Ohmuro-Matsuyama Y, Nakano K, Kimura A,
Ayabe K, Ihara M, Wada T, Ueda H (2013) A
protein-protein interaction assay based on the
functional complementation of mutant firefly
luciferases. Anal Chem 85(16):7935–7940.
doi:10.1021/ac4016825
2. Kurihara M, Ohmuro-Matsuyama Y, Ayabe K,
Yamashita T, Yamaji H, Ueda H (2016) Ultra
sensitive firefly luciferase-based protein-protein
interaction assay (FlimPIA) attained by hinge
region engineering and optimized reaction
conditions. Biotechnol J 11(1):91–99.
doi:10.1002/biot.201500189
3. Ohmuro-Matsuyama Y, Hara Y, Ueda H
(2014) Improved protein-protein interaction
assay FlimPIA by the entrapment of luciferase
conformation. Anal Chem 86(4):2013–2018.
doi:10.1021/ac403065v
4. Kitayama A, Yoshizaki H, Ohmiya Y, Ueda H,
Nagamune T (2003) Creation of a thermostable firefly luciferase with pH-insensitive luminescent
color.
Photochem
Photobiol
77(3):333–338
5. Ayabe K, Zako T, Ueda H (2005) The role of
firefly luciferase C-terminal domain in efficient
coupling of adenylation and oxidative steps.
FEBS
Lett
579(20):4389–4394.
doi:10.1016/j.febslet.2005.07.004
6. Viviani VR, Ohmiya Y (2006) Bovine serum
albumin displays luciferase-like activity in presence of luciferyl adenylate: insights on the origin
of protoluciferase activity and bioluminescence
colours.
Luminescence
21(4):262–267.
doi:10.1002/bio.916
7. Morton RA, Hopkins TA, Seliger HH (1969)
The spectroscopic properties of firefly luciferin
and related compounds. An approach to product emission. Biochemistry 8(4):1598–1607
8. Brown EJ, Albers MW, Shin TB, Ichikawa K,
Keith CT, Lane WS, Schreiber SL (1994) A
mammalian protein targeted by G1-arresting
rapamycin-receptor
complex.
Nature
369(6483):756–758. doi:10.1038/369756a0
9. Chen J, Zheng XF, Brown EJ, Schreiber SL
(1995) Identification of an 11-kDa FKBP12rapamycin- binding domain within the 289kDa FKBP12-rapamycin-associated protein
and characterization of a critical serine residue. Proc Natl Acad Sci U S A 92(11):
4947–4951
10. Chiu MI, Katz H, Berlin V (1994) RAPT1, a
mammalian homolog of yeast Tor, interacts
with the FKBP12/rapamycin complex. Proc
Natl Acad Sci U S A 91(26):12574–12578
11. Branchini BR, Southworth TL, Murtiashaw
MH, Wilkinson SR, Khattak NF, Rosenberg
JC, Zimmer M (2005) Mutagenesis evidence
that the partial reactions of firefly bioluminescence are catalyzed by different conformations
of the luciferase C-terminal domain.
Biochemistry 44(5):1385–1393. doi:10.1021/
bi047903f
12. Gulick AM (2009) Conformational dynamics
in the Acyl-CoA synthetases, adenylation
domains of non-ribosomal peptide synthetases,
and firefly luciferase. ACS Chem Biol
4(10):811–827. doi:10.1021/cb900156h
13. Fujii H, Noda K, Asami Y, Kuroda A, Sakata
M, Tokida A (2007) Increase in bioluminescence intensity of firefly luciferase using genetic
modification. Anal Biochem 366(2):131–136.
doi:10.1016/j.ab.2007.04.018
14. White PJ, Squirrell DJ, Arnaud P, Lowe CR,
Murray JA (1996) Improved thermostability of
the North American firefly luciferase: saturation mutagenesis at position 354. Biochem
J 319(Pt 2):343–350
15. Branchini BR, Murtiashaw MH, Magyar RA,
Anderson SM (2000) The role of lysine 529, a
conserved residue of the acyl-adenylateforming enzyme superfamily, in firefly luciferase.
Biochemistry 39(18):5433–5440
References
Yuki Ohmuro-Matsuyama and Hiroshi Ueda
1. Ohmuro-Matsuyama Y, Nakano K, Kimura A,
Ayabe K, Ihara M, Wada T, Ueda H (2013) A
protein-protein interaction assay based on the
functional complementation of mutant firefly
luciferases. Anal Chem 85(16):7935–7940.
doi:10.1021/ac4016825
2. Kurihara M, Ohmuro-Matsuyama Y, Ayabe K,
Yamashita T, Yamaji H, Ueda H (2016) Ultra
sensitive firefly luciferase-based protein-protein
interaction assay (FlimPIA) attained by hinge
region engineering and optimized reaction
conditions. Biotechnol J 11(1):91–99.
doi:10.1002/biot.201500189
3. Ohmuro-Matsuyama Y, Hara Y, Ueda H
(2014) Improved protein-protein interaction
assay FlimPIA by the entrapment of luciferase
conformation. Anal Chem 86(4):2013–2018.
doi:10.1021/ac403065v
4. Kitayama A, Yoshizaki H, Ohmiya Y, Ueda H,
Nagamune T (2003) Creation of a thermostable firefly luciferase with pH-insensitive luminescent
color.
Photochem
Photobiol
77(3):333–338
5. Ayabe K, Zako T, Ueda H (2005) The role of
firefly luciferase C-terminal domain in efficient
coupling of adenylation and oxidative steps.
FEBS
Lett
579(20):4389–4394.
doi:10.1016/j.febslet.2005.07.004
6. Viviani VR, Ohmiya Y (2006) Bovine serum
albumin displays luciferase-like activity in presence of luciferyl adenylate: insights on the origin
of protoluciferase activity and bioluminescence
colours.
Luminescence
21(4):262–267.
doi:10.1002/bio.916
7. Morton RA, Hopkins TA, Seliger HH (1969)
The spectroscopic properties of firefly luciferin
and related compounds. An approach to product emission. Biochemistry 8(4):1598–1607
8. Brown EJ, Albers MW, Shin TB, Ichikawa K,
Keith CT, Lane WS, Schreiber SL (1994) A
mammalian protein targeted by G1-arresting
rapamycin-receptor
complex.
Nature
369(6483):756–758. doi:10.1038/369756a0
9. Chen J, Zheng XF, Brown EJ, Schreiber SL
(1995) Identification of an 11-kDa FKBP12rapamycin- binding domain within the 289kDa FKBP12-rapamycin-associated protein
and characterization of a critical serine residue. Proc Natl Acad Sci U S A 92(11):
4947–4951
10. Chiu MI, Katz H, Berlin V (1994) RAPT1, a
mammalian homolog of yeast Tor, interacts
with the FKBP12/rapamycin complex. Proc
Natl Acad Sci U S A 91(26):12574–12578
11. Branchini BR, Southworth TL, Murtiashaw
MH, Wilkinson SR, Khattak NF, Rosenberg
JC, Zimmer M (2005) Mutagenesis evidence
that the partial reactions of firefly bioluminescence are catalyzed by different conformations
of the luciferase C-terminal domain.
Biochemistry 44(5):1385–1393. doi:10.1021/
bi047903f
12. Gulick AM (2009) Conformational dynamics
in the Acyl-CoA synthetases, adenylation
domains of non-ribosomal peptide synthetases,
and firefly luciferase. ACS Chem Biol
4(10):811–827. doi:10.1021/cb900156h
13. Fujii H, Noda K, Asami Y, Kuroda A, Sakata
M, Tokida A (2007) Increase in bioluminescence intensity of firefly luciferase using genetic
modification. Anal Biochem 366(2):131–136.
doi:10.1016/j.ab.2007.04.018
14. White PJ, Squirrell DJ, Arnaud P, Lowe CR,
Murray JA (1996) Improved thermostability of
the North American firefly luciferase: saturation mutagenesis at position 354. Biochem
J 319(Pt 2):343–350
15. Branchini BR, Murtiashaw MH, Magyar RA,
Anderson SM (2000) The role of lysine 529, a
conserved residue of the acyl-adenylateforming enzyme superfamily, in firefly luciferase.
Biochemistry 39(18):5433–5440
References
Yuki Ohmuro-Matsuyama and Hiroshi Ueda