117
of effective molarity on linker length for an
intramolecular protein−ligand system. J Am
Chem Soc 129(5):1312–1320. doi:10.1021/
ja066780e
22. Brun MA, Tan K-T, Nakata E, Hinner MJ,
Johnsson K (2009) Semisynthetic fluorescent
sensor proteins based on self-labeling protein
tags. J Am Chem Soc 131(16):5873–5884.
doi:10.1021/ja900149e
23. Evers TH, van Dongen EMWM, Faesen AC,
Meijer EW, Merkx M (2006) Quantitative
understanding of the energy transfer between
fluorescent proteins connected via flexible peptide linkers. Biochemistry 45(44):13183–
13192. doi:10.1021/bi061288t
24. Schuler B, Lipman EA, Steinbach PJ, Kumke
M, Eaton WA (2005) Polyproline and the
“spectroscopic ruler” revisited with singlemolecule fluorescence. Proc Natl Acad Sci
USA
102(8):2754–2759.
doi:10.1073/
pnas.0408164102
25. Brun MA, Griss R, Reymond L, Tan K-T, Piguet
J, Peters RJRW, Vogel H, Johnsson K (2011)
Semisynthesis of fluorescent metabolite sensors
on cell surfaces. J Am Chem Soc 133(40):16235–
16242. doi:10.1021/ja206915m
26. Gibson DG, Young L, Chuang R-Y, Venter JC,
Hutchison CA, Smith HO (2009) Enzymatic
assembly of DNA molecules up to several hundred kilobases. Nat Methods 6(5):343–345.
http://www.nature.com/nmeth/journal/
v6/n5/suppinfo/nmeth.1318_S1.html
27. Brun MA, Tan K-T, Griss R, Kielkowska A,
Reymond L, Johnsson K (2012) A semisynthetic fluorescent sensor protein for glutamate.
J Am Chem Soc 134(18):7676–7678.
doi:10.1021/ja3002277
28. Masharina A, Reymond L, Maurel D, Umezawa
K, Johnsson K (2012) A fluorescent sensor for
GABA and synthetic GABAB receptor ligands.
J Am Chem Soc 134(46):19026–19034.
doi:10.1021/ja306320s
29. Schena A, Johnsson K (2014) Sensing acetylcholine and anticholinesterase compounds.
Angew Chem Int Ed 53(5):1302–1305.
doi:10.1002/anie.201307754
30. Griss R, Schena A, Reymond L, Patiny L,
Werner D, Tinberg CE, Baker D, Johnsson K
(2014) Bioluminescent sensor proteins for
point-of-care therapeutic drug monitoring.
Nat Chem Biol 10(7):598–603. doi:10.1038/
nchembio.1554
SNIFITS and LUCIDs: Semi-Synthetic Modular Biosensors
of effective molarity on linker length for an
intramolecular protein−ligand system. J Am
Chem Soc 129(5):1312–1320. doi:10.1021/
ja066780e
22. Brun MA, Tan K-T, Nakata E, Hinner MJ,
Johnsson K (2009) Semisynthetic fluorescent
sensor proteins based on self-labeling protein
tags. J Am Chem Soc 131(16):5873–5884.
doi:10.1021/ja900149e
23. Evers TH, van Dongen EMWM, Faesen AC,
Meijer EW, Merkx M (2006) Quantitative
understanding of the energy transfer between
fluorescent proteins connected via flexible peptide linkers. Biochemistry 45(44):13183–
13192. doi:10.1021/bi061288t
24. Schuler B, Lipman EA, Steinbach PJ, Kumke
M, Eaton WA (2005) Polyproline and the
“spectroscopic ruler” revisited with singlemolecule fluorescence. Proc Natl Acad Sci
USA
102(8):2754–2759.
doi:10.1073/
pnas.0408164102
25. Brun MA, Griss R, Reymond L, Tan K-T, Piguet
J, Peters RJRW, Vogel H, Johnsson K (2011)
Semisynthesis of fluorescent metabolite sensors
on cell surfaces. J Am Chem Soc 133(40):16235–
16242. doi:10.1021/ja206915m
26. Gibson DG, Young L, Chuang R-Y, Venter JC,
Hutchison CA, Smith HO (2009) Enzymatic
assembly of DNA molecules up to several hundred kilobases. Nat Methods 6(5):343–345.
http://www.nature.com/nmeth/journal/
v6/n5/suppinfo/nmeth.1318_S1.html
27. Brun MA, Tan K-T, Griss R, Kielkowska A,
Reymond L, Johnsson K (2012) A semisynthetic fluorescent sensor protein for glutamate.
J Am Chem Soc 134(18):7676–7678.
doi:10.1021/ja3002277
28. Masharina A, Reymond L, Maurel D, Umezawa
K, Johnsson K (2012) A fluorescent sensor for
GABA and synthetic GABAB receptor ligands.
J Am Chem Soc 134(46):19026–19034.
doi:10.1021/ja306320s
29. Schena A, Johnsson K (2014) Sensing acetylcholine and anticholinesterase compounds.
Angew Chem Int Ed 53(5):1302–1305.
doi:10.1002/anie.201307754
30. Griss R, Schena A, Reymond L, Patiny L,
Werner D, Tinberg CE, Baker D, Johnsson K
(2014) Bioluminescent sensor proteins for
point-of-care therapeutic drug monitoring.
Nat Chem Biol 10(7):598–603. doi:10.1038/
nchembio.1554
SNIFITS and LUCIDs: Semi-Synthetic Modular Biosensors