74
4 Analytical Techniques
(continued)
References
Species used
Analytical methodology
used
Components Analysed
Metals analysed
Instrument used for
analysis
Medina-Elizade et al.
(2002)
Montastraea annularis
Samples dissolved in
nitric acid and chelated
using APDC/MIBK
Skeleton
Pb
Atomic Absorption
Spectroscopy (AAS)
Mokhtar et al. (2002)
Porites spp
Favia spp
Nitric acid
H2O2: NaOH (1:1)
Coral skeletons
Cr
Mn
Hg
Zn
Neutron Activation
Analysis (NAA)
Reichelt-Brushett and
McOrist (2003)
Acropora tenius
Separation of tissue and
zooxanthellae from
skeleton
Acid digestion of all
components using 1:1
nitric acid and peroxide
on a hot plate for 3hrs
Zooxanthellae
Tissue
Skeleton
Pb
Cd
Zn
Cu
Fe
Ni
Mn
As
Neutron Activation
Analysis (NAA) and
Inductively Coupled
Plasma Mass
Spectroscopy (ICPMS)
Smith et al.(2003)
Acropora formosa
Acropora microphthalma
Butyltins extracted using
methanol/HCl
Volatile tin hydrides
formed and packed in
columns
Cu/Zn-acid digested
Coral branches were
sediment treated
TBT(Sn)
DBT(Sn)
Mbt(Sn)
Graphite Furnace Atomic
Absorption
Spectrophotometry
(GFAAS)
Cu
Zn
Inductively Coupled
Plasma-Atomic Emission
Spectroscopy (ICP-AES)
Bastidas and Garcia
(2004)
Porites astreoides
Acid digestion for 5 h in
a water bath
Polyp, zooxanthellae and
skeleton
Hg
Cold Vapor Atomic
Absorption Spectroscopy
(CVAAS)
(continued)