60
C. Rochette-Egly
Fig. 3.1 Chronology of the main technologic advances in the field of the RA-regulated genes during
cell differentiation and development
1990, 1991). However, as the screenings were performed relatively late after RA
addition (24–48 h), it was not possible to discriminate whether the identified genes
were direct or indirect RA targets.
A few years later, additional novel genes rapidly up regulated by RA were isolated, using a sensitive subtractive hybridization strategy based on biotin-streptavidin
affinity and polymerase chain reaction (PCR) (Bouillet et al. 1995b; Fig. 3.1 and
C. Rochette-Egly
Fig. 3.1 Chronology of the main technologic advances in the field of the RA-regulated genes during
cell differentiation and development
1990, 1991). However, as the screenings were performed relatively late after RA
addition (24–48 h), it was not possible to discriminate whether the identified genes
were direct or indirect RA targets.
A few years later, additional novel genes rapidly up regulated by RA were isolated, using a sensitive subtractive hybridization strategy based on biotin-streptavidin
affinity and polymerase chain reaction (PCR) (Bouillet et al. 1995b; Fig. 3.1 and