Current Applications of Artificial
Metalloenzymes and Future
Developments
Jean-Pierre Mahy, Frédéric Avenier, Wadih Ghattas, Rémy Ricoux,
and Michèle Salmain
Abstract
In between traditional homogeneous metal catalysts and enzyme catalysts, a new
class of hybrid catalysts named artificial metalloenzymes resulting from the
controlled embedding of transition metal species (ions, synthetic inorganic or
organometallic complexes) within natural, genetically-engineered or even de
novo protein scaffolds currently undergoes a tremendous development at the
academic level. This family of hybrid assemblies ideally combines the features
of their individual components, allowing a wide range of chemical reactions,
including new-to-nature reactions, to be catalyzed under mild, eco-compatible
conditions with high chemo- and/or stereoselectivity. This chapter intends to
summarize the most remarkable achievements in artificial metalloenzyme design
and properties with emphasis put on industrially relevant chemical reactions,
including oxidations, imine reductions, C–C and C–N bonds formation. It also
gives an up-to-date survey on the most advanced applications of artificial
metalloenzymes in cascade reactions and in vivo catalysis.
Keywords
Oxidation Á Reduction Á Polymerization Á Metathesis Á Cascade reactions Á
In vivo catalysis Á Uncaging Á Carbene insertion Á Directed evolution
J.-P. Mahy Á F. Avenier Á W. Ghattas Á R. Ricoux
Institut de Chimie Moléculaire et des Matériaux d’Orsay (ICMMO), UMR 8182, CNRS,
Université Paris Sud, Université Paris-Saclay, Orsay, France
M. Salmain (&)
Sorbonne Université, CNRS, Institut Parisien de Chimie Moléculaire (IPCM), Paris, France
e-mail: michele.salmain@sorbonne-universite.fr
© Springer Nature Switzerland AG 2021
J. J. G. Moura et al. (eds.), Enzymes for Solving Humankind's Problems,
https://doi.org/10.1007/978-3-030-58315-6_12
363
Metalloenzymes and Future
Developments
Jean-Pierre Mahy, Frédéric Avenier, Wadih Ghattas, Rémy Ricoux,
and Michèle Salmain
Abstract
In between traditional homogeneous metal catalysts and enzyme catalysts, a new
class of hybrid catalysts named artificial metalloenzymes resulting from the
controlled embedding of transition metal species (ions, synthetic inorganic or
organometallic complexes) within natural, genetically-engineered or even de
novo protein scaffolds currently undergoes a tremendous development at the
academic level. This family of hybrid assemblies ideally combines the features
of their individual components, allowing a wide range of chemical reactions,
including new-to-nature reactions, to be catalyzed under mild, eco-compatible
conditions with high chemo- and/or stereoselectivity. This chapter intends to
summarize the most remarkable achievements in artificial metalloenzyme design
and properties with emphasis put on industrially relevant chemical reactions,
including oxidations, imine reductions, C–C and C–N bonds formation. It also
gives an up-to-date survey on the most advanced applications of artificial
metalloenzymes in cascade reactions and in vivo catalysis.
Keywords
Oxidation Á Reduction Á Polymerization Á Metathesis Á Cascade reactions Á
In vivo catalysis Á Uncaging Á Carbene insertion Á Directed evolution
J.-P. Mahy Á F. Avenier Á W. Ghattas Á R. Ricoux
Institut de Chimie Moléculaire et des Matériaux d’Orsay (ICMMO), UMR 8182, CNRS,
Université Paris Sud, Université Paris-Saclay, Orsay, France
M. Salmain (&)
Sorbonne Université, CNRS, Institut Parisien de Chimie Moléculaire (IPCM), Paris, France
e-mail: michele.salmain@sorbonne-universite.fr
© Springer Nature Switzerland AG 2021
J. J. G. Moura et al. (eds.), Enzymes for Solving Humankind's Problems,
https://doi.org/10.1007/978-3-030-58315-6_12
363