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124. Skoronski E, Souza DH, Ely C et al (2017) Immobilization of laccase from Aspergillus
oryzae on graphene nanosheets. Int J Biol Macromol 99:121–127. https://doi.org/10.1016/j.
ijbiomac.2017.02.076
125. Santucci R, Ferri T, Morpurgo L et al (1998) Unmediated heterogeneous electron transfer
reaction of ascorbate oxidase and laccase at a gold electrode. Biochem J 332:611–615.
https://doi.org/10.1042/bj3320611
126. Akyilmaz E, Dinçkaya E (1999) A new enzyme electrode based on ascorbate oxidase
immobilized in gelatin for specific determination of l-ascorbic acid. Talanta 50:87–93.
https://doi.org/10.1016/S0039-9140(99)00107-1
127. Wang X, Watanabe H, Uchiyama S (2008) Amperometric l-ascorbic acid biosensors
equipped with enzyme micelle membrane. Talanta 74:1681–1685. https://doi.org/10.1016/j.
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128. Chauhan N, Dahiya T, Priyanka Pundir CS (2010) Fabrication of an amperometric ascorbate
biosensor using egg shell membrane bound Lagenaria siceraria fruit ascorbate oxidase. J Mol
Catal B Enzym 67:66–71. https://doi.org/10.1016/j.molcatb.2010.07.007
129. Zhang M, Liu K, Xiang L et al (2007) Carbon Nanotube-Modified Carbon Fiber
Microelectrodes for In Vivo Voltammetric Measurement of Ascorbic Acid in Rat Brain.
Anal Chem 79:6559–6565. https://doi.org/10.1021/ac0705871
130. Rekha K, Gouda MD, Thakur MS, Karanth NG (2000) Ascorbate oxidase based
amperometric biosensor for organophosphorous pesticide monitoring. Biosens Bioelectron
15:499–502. https://doi.org/10.1016/S0956-5663(00)00077-4
131. Pisoschi AM, Pop A, Serban AI, Fafaneata C (2014) Electrochemical methods for ascorbic
acid determination. Electrochim Acta 121:443–460. https://doi.org/10.1016/j.electacta.2013.
12.127
132. Tanaka N, Murao S (1983) Difference between Various Copper-containing Enzymes
(Polyporus Laccase, Mushroom Tyrosinase and Cucumber Ascorbate Oxidase) and Bilirubin
Oxidase. Agric Biol Chem 47:1627–1628. https://doi.org/10.1271/bbb1961.47.1627
133. Mizutani K, Toyoda M, Sagara K et al (2010) X-ray analysis of bilirubin oxidase from
Myrothecium verrucaria at 2.3 Å resolution using a twinned crystal. Acta Crystallogr, Sect
F: Struct Biol Cryst Commun 66:765–770. https://doi.org/10.1107/S1744309110018828
134. Mano N (2012) Features and applications of bilirubin oxidases. Appl Microbiol Biotechnol
96:301–307. https://doi.org/10.1007/s00253-012-4312-9
135. Mano N, Edembe L (2013) Bilirubin oxidases in bioelectrochemistry: Features and recent
findings. Biosens Bioelectron 50:478–485. https://doi.org/10.1016/j.bios.2013.07.014
136. Tonda-Mikiela P, Habrioux A, Boland S et al (2011) Oxygen Electroreduction Catalyzed by
Bilirubin Oxidase Does Not Release Hydrogen Peroxide. Electrocatalysis 2:268–272. https://
doi.org/10.1007/s12678-011-0062-1
137. Tsujimura S, Kano K, Ikeda T (2005) Bilirubin oxidase in multiple layers catalyzes
four-electron reduction of dioxygen to water without redox mediators. J Electroanal Chem
576:113–120. https://doi.org/10.1016/j.jelechem.2004.09.031
138. Shleev S, El Kasmi A, Ruzgas T, Gorton L (2004) Direct heterogeneous electron transfer
reactions of bilirubin oxidase at a spectrographic graphite electrode. Electrochem Commun
6:934–939. https://doi.org/10.1016/j.elecom.2004.07.008
139. Brocato S, Lau C, Atanassov P (2012) Mechanistic study of direct electron transfer in
bilirubin oxidase. Electrochim Acta 61:44–49. https://doi.org/10.1016/j.electacta.2011.11.
074
140. Li D an, Okajima T, Mao L, Ohsaka T (2014) Bioelectrocatalytic oxygen reduction reaction
by bilirubin oxidase adsorbed on glassy carbon and edge-plane pyrolytic graphite electrodes:
Effect of redox mediators. Int J Electrochem Sci 9:1390–1398
352
T. Monteiro et al.
oxidase immobilized on a nanostructured gold electrode. Bioelectrochemistry 94:69–74.
https://doi.org/10.1016/j.bioelechem.2013.07.001
124. Skoronski E, Souza DH, Ely C et al (2017) Immobilization of laccase from Aspergillus
oryzae on graphene nanosheets. Int J Biol Macromol 99:121–127. https://doi.org/10.1016/j.
ijbiomac.2017.02.076
125. Santucci R, Ferri T, Morpurgo L et al (1998) Unmediated heterogeneous electron transfer
reaction of ascorbate oxidase and laccase at a gold electrode. Biochem J 332:611–615.
https://doi.org/10.1042/bj3320611
126. Akyilmaz E, Dinçkaya E (1999) A new enzyme electrode based on ascorbate oxidase
immobilized in gelatin for specific determination of l-ascorbic acid. Talanta 50:87–93.
https://doi.org/10.1016/S0039-9140(99)00107-1
127. Wang X, Watanabe H, Uchiyama S (2008) Amperometric l-ascorbic acid biosensors
equipped with enzyme micelle membrane. Talanta 74:1681–1685. https://doi.org/10.1016/j.
talanta.2007.09.008
128. Chauhan N, Dahiya T, Priyanka Pundir CS (2010) Fabrication of an amperometric ascorbate
biosensor using egg shell membrane bound Lagenaria siceraria fruit ascorbate oxidase. J Mol
Catal B Enzym 67:66–71. https://doi.org/10.1016/j.molcatb.2010.07.007
129. Zhang M, Liu K, Xiang L et al (2007) Carbon Nanotube-Modified Carbon Fiber
Microelectrodes for In Vivo Voltammetric Measurement of Ascorbic Acid in Rat Brain.
Anal Chem 79:6559–6565. https://doi.org/10.1021/ac0705871
130. Rekha K, Gouda MD, Thakur MS, Karanth NG (2000) Ascorbate oxidase based
amperometric biosensor for organophosphorous pesticide monitoring. Biosens Bioelectron
15:499–502. https://doi.org/10.1016/S0956-5663(00)00077-4
131. Pisoschi AM, Pop A, Serban AI, Fafaneata C (2014) Electrochemical methods for ascorbic
acid determination. Electrochim Acta 121:443–460. https://doi.org/10.1016/j.electacta.2013.
12.127
132. Tanaka N, Murao S (1983) Difference between Various Copper-containing Enzymes
(Polyporus Laccase, Mushroom Tyrosinase and Cucumber Ascorbate Oxidase) and Bilirubin
Oxidase. Agric Biol Chem 47:1627–1628. https://doi.org/10.1271/bbb1961.47.1627
133. Mizutani K, Toyoda M, Sagara K et al (2010) X-ray analysis of bilirubin oxidase from
Myrothecium verrucaria at 2.3 Å resolution using a twinned crystal. Acta Crystallogr, Sect
F: Struct Biol Cryst Commun 66:765–770. https://doi.org/10.1107/S1744309110018828
134. Mano N (2012) Features and applications of bilirubin oxidases. Appl Microbiol Biotechnol
96:301–307. https://doi.org/10.1007/s00253-012-4312-9
135. Mano N, Edembe L (2013) Bilirubin oxidases in bioelectrochemistry: Features and recent
findings. Biosens Bioelectron 50:478–485. https://doi.org/10.1016/j.bios.2013.07.014
136. Tonda-Mikiela P, Habrioux A, Boland S et al (2011) Oxygen Electroreduction Catalyzed by
Bilirubin Oxidase Does Not Release Hydrogen Peroxide. Electrocatalysis 2:268–272. https://
doi.org/10.1007/s12678-011-0062-1
137. Tsujimura S, Kano K, Ikeda T (2005) Bilirubin oxidase in multiple layers catalyzes
four-electron reduction of dioxygen to water without redox mediators. J Electroanal Chem
576:113–120. https://doi.org/10.1016/j.jelechem.2004.09.031
138. Shleev S, El Kasmi A, Ruzgas T, Gorton L (2004) Direct heterogeneous electron transfer
reactions of bilirubin oxidase at a spectrographic graphite electrode. Electrochem Commun
6:934–939. https://doi.org/10.1016/j.elecom.2004.07.008
139. Brocato S, Lau C, Atanassov P (2012) Mechanistic study of direct electron transfer in
bilirubin oxidase. Electrochim Acta 61:44–49. https://doi.org/10.1016/j.electacta.2011.11.
074
140. Li D an, Okajima T, Mao L, Ohsaka T (2014) Bioelectrocatalytic oxygen reduction reaction
by bilirubin oxidase adsorbed on glassy carbon and edge-plane pyrolytic graphite electrodes:
Effect of redox mediators. Int J Electrochem Sci 9:1390–1398
352
T. Monteiro et al.