Table 1
(continued)
Enzyme family
EC
number
Enzyme
Cofactors
Source examples
Reduction potentials/ E
°
′ vs.
NHE
References
Reductases
1.7.1.1
Eukaryotic NaR
Molybdenum
(active site), heme
b,
FAD
Arabidopsis thaliana
−300 to 15 mV range (all
cofactors)
[77]
1.7.5.1
Respiratory NaR
Molybdenum
(active site), heme
b,
[4Fe-4S],
[3Fe-4S]
Escherichia coli
200 mV (Mo(VI/V)) and
100 mV (Mo(V/IV))
[78]
1.7.7.2
Dissimilatory
NaR
Molybdenum
(active site), heme
b,
[4Fe-4S]
Rhodobacter
sphaeroides
550 mV (Mo(VI/V)) and
−225 mV (Mo(V/IV))
[78]
1.7.1.4
Assimilatory
NaR
Molybdenum
(active site), heme
b,
[4Fe-4S]
Paracoccus
denitrificans
–
–
1.7.7.1
Sirohemic NiR
siroheme (active
site), [4Fe-4S]
Spinacea oleracea
−290 mV (active site)
[79]
1.7.2.2
Cytochrome
c
NiR
penta-coordinated
heme c
(active site),
hexa-coordinated
heme c
Desulfovibrio
desulfuricans
−80 mV (active site)
[80]
1.7.2.1
Copper NiR
Type I and Type II
(active site) copper
Rhodobacter
sphaeroides
218 mV (active site, pH 6)
[81]
1.7.2.1
Cytochrome cd
1
NiR
heme c,
heme d
1
(active site)
Marinobacter
hydrocarbonoclasticus
199 mV (active site, pH 7.6)
[82]
1.7.2.5
NO reductase
heme b
3 /non-heme
FeB di-iron center
Marinobacter
hydrocarbonoclasticus
−393 mV (active site)
[83]
(continued)
Selective Enzymes at the Core of Advanced Electroanalytical …
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