Table 1
Examples of oxidoreductase enzymes used in biosensors
Enzyme family
EC
number
Enzyme
Cofactors
Source examples
Reduction potentials/ E
°
′ vs.
NHE
References
Oxidases
1.1.3.4
Glucose oxidase
FAD
Aspergillus niger
−99 mV (pH 7.6)
[62]
1.17.3.2
Xanthine
oxidase
Molybdenum
(active site),
[2Fe-2S], FAD
Bovine milk
−343 mV (Mo(VI/V)),
−362 mV (Mo(V/IV))
[63]
1.1.3.17
Choline oxidase
FAD
Alcaligenes sp.
−185 and
−195 mV
[57]
1.1.3.6
Cholesterol
oxidase
FAD
Streptomyces
hygroscopicus
−217 mV (pH 7.5)
[64]
1.1.3.6
Cholesterol
oxidase
FAD
Brevibacterium
sterolicum
−101 mV (pH 7.5)
[64]
1.1.3.2
Lactate oxidase
FMN
Aerococcus viridans
−128 mV
[65]
1.10.3.2
Laccase
Type I and type
II/III trinuclear
copper
Trametes versicolor
520 mV (TI), 197 mV (TII),
360 mV (TIII), pH 5.8
[66]
1.10.3.3
Ascorbate
oxidase
Type I and type
II/III trinuclear
copper
Cucurbita sp.
420 mV (TI), 153 mV (TII), pH
6.8
[66]
1.3.3.5
Bilirubin
oxidase
Type I and type
II/III trinuclear
copper
Myrothecium
verrucaria
546 mV (TI), 217 mV (TII),
385 mV (TIII), pH 5.8
[66]
(continued)
Selective Enzymes at the Core of Advanced Electroanalytical …
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