49. Mania D, Heylen K, van Spanning RJM et al (2016) Regulation of nitrogen metabolism in
the nitrate-ammonifying soil bacterium Bacillus vireti and evidence for its ability to grow
using N 2 O as electron acceptor. Environ Microbiol 18:2937–2950
50. Domeignoz-Horta LA, Putz M, Spor A et al (2016) Non-denitrifying nitrous oxide-reducing
bacteria—an effective N 2 O sink in soil. Soil Biol Biochem 103:376–379
51. Kern M, Simon J (2016) Three transcription regulators of the Nss family mediate the
adaptive response induced by nitrate, nitric oxide or nitrous oxide in Wolinella succinogenes.
Environ Microbiol 18:2899–2912
52. Zheng M, He D, Ma T et al (2014) Reducing NO and N 2 O emission during aerobic
denitrification by newly isolated Pseudomonas stutzeri PCN-1. Bioresour Technol 162:80–88
53. Desloover J, Roosbroeck D, Heylen K et al (2014) Pathway of nitrous oxide consumption in
isolated Pseudomonas stutzeri strains under anoxic and oxic conditions. Environ Microbiol
16:3143–3152
54. Zhao B, Chen DY, Tan P et al (2018) Characterization of an aerobic denitrifier Pseudomonas
stutzeri strain XL-2 to achieve efficient nitrate removal. Bioresour Technol 250:564–573
55. Miyahara M, Kim SW, Fushinobu S et al (2010) Potential of aerobic denitrification by
Pseudomonas stutzeri TR2 to reduce nitrous oxide emissions from wastewater treatment
plants. Appl Environ Microbiol 76:4619–4625
56. Miyahara M, Kim SW, Zhou S et al (2012) Survival of the aerobic denitrifier Pseudomonas
stutzeri strain TR2 during co-culture with activated sludge under denitrifying conditions.
Biosci Biotechnol Biochem 76:495–500
57. Chee-Sanford J, Tian D, Sanford R (2020) Consumption of N 2 O and other N-cycle
intermediates by Gemmatimonas aurantiaca strain T-27. Microbiology 165:1345–1354
58. Simon J, Einsle O, Kroneck PMH et al (2004) The unprecedented nos gene cluster of
Wolinella succinogenes encodes a novel respiratory electron transfer pathway to cytochrome
c nitrous oxide reductase. FEBS Lett 569:7–12
59. Dell’Acqua S, Moura I, Moura JJG et al (2011) The electron transfer complex between
nitrous oxide reductase and its electron donors. J Biol Inorg Chem 16:1241–1254
60. Stevens JM, Mavridou DA, Hamer R et al (2011) Cytochrome c biogenesis System I.
FEBS J 278:4170–4178
61. Simon J, Hederstedt L (2011) Composition and function of cytochrome c biogenesis
System II. FEBS J 278:4179–4188
62. Teraguchi S, Hollocher TC (1989) Purification and some characteristics of a cytochrome ccontaining nitrous oxide reductase from Wolinella succinogenes. J Biol Chem 264:1972–
1979
63. Brown K, Tegoni M, Prudencio M et al (2000) A novel type of catalytic copper cluster in
nitrous oxide reductase. Nat Struct Biol 7:191–195
64. Haltia T, Brown K, Tegoni M et al (2003) Crystal structure of nitrous oxide reductase from
Paracoccus denitrificans at 1.6 Å resolution. Biochem J 369:77–88
65. Paraskevopoulos K, Antonyuk SV, Sawers G et al (2006) Insight into catalysis of nitrous
oxide reductase from high-resolution structures of resting and inhibitor-bound enzyme from
Achromobacter cycloclastes. J Mol Biol 362:55–65
66. Pomowski A, Zumft WG, Kroneck PMH et al (2011) N 2 O binding at a [4Cu:2S]
copper-sulphur cluster in nitrous oxide reductase. Nature 477:234–237
67. Pauleta SR, Dell’Acqua S, Moura I (2013) Nitrous oxide reductase. Coord Chem Rev
257:332–349
68. Carreira C, Pauleta SR, Moura I (2017) The catalytic cycle of nitrous oxide reductase—the
enzyme that catalyzes the last step of denitrification. J Inorg Biochem 177:423–434
69. Zumft WG, Kroneck PMH (2007) Respiratory transformation of nitrous oxide (N 2 O) to
dinitrogen by Bacteria and Archaea. Adv Microb Physiol 52:107–227
70. van Spanning RJM (2011) Structure, function, regulation and evolution of the nitrite and
nitrous oxide reductases: denitrification enzymes with a beta-propeller fold. In: Moir JWB
(ed) Nitrogen cycling in bacteria. Caister Academic Press, Norfolk, pp 135–161
208
J. Simon
the nitrate-ammonifying soil bacterium Bacillus vireti and evidence for its ability to grow
using N 2 O as electron acceptor. Environ Microbiol 18:2937–2950
50. Domeignoz-Horta LA, Putz M, Spor A et al (2016) Non-denitrifying nitrous oxide-reducing
bacteria—an effective N 2 O sink in soil. Soil Biol Biochem 103:376–379
51. Kern M, Simon J (2016) Three transcription regulators of the Nss family mediate the
adaptive response induced by nitrate, nitric oxide or nitrous oxide in Wolinella succinogenes.
Environ Microbiol 18:2899–2912
52. Zheng M, He D, Ma T et al (2014) Reducing NO and N 2 O emission during aerobic
denitrification by newly isolated Pseudomonas stutzeri PCN-1. Bioresour Technol 162:80–88
53. Desloover J, Roosbroeck D, Heylen K et al (2014) Pathway of nitrous oxide consumption in
isolated Pseudomonas stutzeri strains under anoxic and oxic conditions. Environ Microbiol
16:3143–3152
54. Zhao B, Chen DY, Tan P et al (2018) Characterization of an aerobic denitrifier Pseudomonas
stutzeri strain XL-2 to achieve efficient nitrate removal. Bioresour Technol 250:564–573
55. Miyahara M, Kim SW, Fushinobu S et al (2010) Potential of aerobic denitrification by
Pseudomonas stutzeri TR2 to reduce nitrous oxide emissions from wastewater treatment
plants. Appl Environ Microbiol 76:4619–4625
56. Miyahara M, Kim SW, Zhou S et al (2012) Survival of the aerobic denitrifier Pseudomonas
stutzeri strain TR2 during co-culture with activated sludge under denitrifying conditions.
Biosci Biotechnol Biochem 76:495–500
57. Chee-Sanford J, Tian D, Sanford R (2020) Consumption of N 2 O and other N-cycle
intermediates by Gemmatimonas aurantiaca strain T-27. Microbiology 165:1345–1354
58. Simon J, Einsle O, Kroneck PMH et al (2004) The unprecedented nos gene cluster of
Wolinella succinogenes encodes a novel respiratory electron transfer pathway to cytochrome
c nitrous oxide reductase. FEBS Lett 569:7–12
59. Dell’Acqua S, Moura I, Moura JJG et al (2011) The electron transfer complex between
nitrous oxide reductase and its electron donors. J Biol Inorg Chem 16:1241–1254
60. Stevens JM, Mavridou DA, Hamer R et al (2011) Cytochrome c biogenesis System I.
FEBS J 278:4170–4178
61. Simon J, Hederstedt L (2011) Composition and function of cytochrome c biogenesis
System II. FEBS J 278:4179–4188
62. Teraguchi S, Hollocher TC (1989) Purification and some characteristics of a cytochrome ccontaining nitrous oxide reductase from Wolinella succinogenes. J Biol Chem 264:1972–
1979
63. Brown K, Tegoni M, Prudencio M et al (2000) A novel type of catalytic copper cluster in
nitrous oxide reductase. Nat Struct Biol 7:191–195
64. Haltia T, Brown K, Tegoni M et al (2003) Crystal structure of nitrous oxide reductase from
Paracoccus denitrificans at 1.6 Å resolution. Biochem J 369:77–88
65. Paraskevopoulos K, Antonyuk SV, Sawers G et al (2006) Insight into catalysis of nitrous
oxide reductase from high-resolution structures of resting and inhibitor-bound enzyme from
Achromobacter cycloclastes. J Mol Biol 362:55–65
66. Pomowski A, Zumft WG, Kroneck PMH et al (2011) N 2 O binding at a [4Cu:2S]
copper-sulphur cluster in nitrous oxide reductase. Nature 477:234–237
67. Pauleta SR, Dell’Acqua S, Moura I (2013) Nitrous oxide reductase. Coord Chem Rev
257:332–349
68. Carreira C, Pauleta SR, Moura I (2017) The catalytic cycle of nitrous oxide reductase—the
enzyme that catalyzes the last step of denitrification. J Inorg Biochem 177:423–434
69. Zumft WG, Kroneck PMH (2007) Respiratory transformation of nitrous oxide (N 2 O) to
dinitrogen by Bacteria and Archaea. Adv Microb Physiol 52:107–227
70. van Spanning RJM (2011) Structure, function, regulation and evolution of the nitrite and
nitrous oxide reductases: denitrification enzymes with a beta-propeller fold. In: Moir JWB
(ed) Nitrogen cycling in bacteria. Caister Academic Press, Norfolk, pp 135–161
208
J. Simon