6. Müller V (2008) Bacterial fermentation. In: Encyclopedia of life sciences. John Wiley &
Sons Ltd., Chichester
7. Ragsdale SW, Pierce E (2008) Acetogenesis and the Wood-Ljungdahl pathway of CO 2
fixation. Biochim Biophys Acta 1784:1873–1898
8. Bengelsdorf FR, Beck MH, Erz C, Hoffmeister S, Karl MM, Riegler P, Wirth S, Poehlein A,
Weuster-Botz D, Dürre P (2018) Bacterial anaerobic synthesis gas (syngas) and CO 2 +H 2
fermentation. Adv Appl Microbiol 103:143–221
9. Munasinghe PC, Khanal SK (2010) Biomass-derived syngas fermentation into biofuels:
opportunities and challenges. Bioresour Technol 101:5013–5022
10. Köpke M, Held C, Hujer S, Liesegang H, Wiezer A, Wollherr A, Ehrenreich A, Liebl W,
Gottschalk G, Dürre P (2010) Clostridium ljungdahlii represents a microbial production
platform based on syngas. Proc Natl Acad Sci USA 107:13087–13092
11. Dürre P, Eikmanns BJ (2015) C1-carbon sources for chemical and fuel production by
microbial gas fermentation. Curr Opin Biotechnol 35:63–72
12. Humphreys CM, Minton NP (2018) Advances in metabolic engineering in the microbial
production of fuels and chemicals from C1 gas. Curr Opin Biotechnol 50:174–181
13. Müller V (2019) New horizons in acetogenic conversion of one-carbon substrates and
biological hydrogen storage. Trends Biotechnol 37:1344–1354
14. Schwarz FM, Schuchmann K, Müller V (2018) Hydrogenation of CO 2 at ambient pressure
catalyzed by a highly active thermostable biocatalyst. Biotechnol Biofuels 11:237
15. Schuchmann K, Müller V (2013) Direct and reversible hydrogenation of CO 2 to formate by
a bacterial carbon dioxide reductase. Science 342:1382–1385
16. Müller V, Inkamp F, Rauwolf A, Küsel K, Drake HL (2004) Molecular and cellular biology
of acetogenic bacteria. In: Nakano M, Zuber P (eds) Strict and facultative anaerobes: medical
and environmental aspects. Horizon Scientific Press, Norfolk, pp 251–281
17. Poehlein A, Cebulla M, Ilg MM, Bengelsdorf FR, Schiel-Bengelsdorf B, Whited G,
Andreesen JR, Gottschalk G, Daniel R, Dürre P (2015) The complete genome sequence of
Clostridium aceticum: a missing link between Rnf- and cytochrome-containing autotrophic
acetogens. mBio 6:e01168–01115
18. Schuchmann K, Müller V (2014) Autotrophy at the thermodynamic limit of life: a model for
energy conservation in acetogenic bacteria. Nat Rev Microbiol 12:809–821
19. Wang S, Huang H, Kahnt J, Müller AP, Köpke M, Thauer RK (2013) NADP-specific
electron-bifurcating [FeFe]-hydrogenase in a functional complex with formate dehydrogenase in Clostridium autoethanogenum grown on CO. J Bacteriol 195:4373–4386
20. Yamamoto I, Saiki T, Liu SM, Ljungdahl LG (1983) Purification and properties of
NADP-dependent formate dehydrogenase from Clostridium thermoaceticum, a
tungsten-selenium-iron protein. J Biol Chem 258:1826–1832
21. Maia LB, Moura JJ, Moura I (2015) Molybdenum and tungsten-dependent formate
dehydrogenases. J Biol Inorg Chem 20:287–309
22. O’Brien WE, Brewer JM, Ljungdahl LG (1973) Purification and characterization of
thermostable 5,10-methylenetetrahydrofolate dehydrogenase from Clostridium thermoaceticum. J Biol Chem 248:403–408
23. Ragsdale SW, Ljungdahl LG (1984) Purification and properties of NAD-dependent
5,10-methylenetetrahydrofolate dehydrogenase from Acetobacterium woodii. J Biol Chem
259:3499–3503
24. Moore MR, O’Brien WE, Ljungdahl LG (1974) Purification and characterization of
nicotinamide adenine dinucleotide-dependent methylenetetrahydrofolate dehydrogenase
from Clostridium formicoaceticum. J Biol Chem 249:5250–5253
25. Wohlfarth G, Geerligs G, Diekert G (1990) Purification and properties of a
NADH-dependent 5,10-methylenetetrahydrofolate reductase from Peptostreptococcus productus. Eur J Biochem 192:411–417
Acetogenic Bacteria for Biotechnological Applications
125
Sons Ltd., Chichester
7. Ragsdale SW, Pierce E (2008) Acetogenesis and the Wood-Ljungdahl pathway of CO 2
fixation. Biochim Biophys Acta 1784:1873–1898
8. Bengelsdorf FR, Beck MH, Erz C, Hoffmeister S, Karl MM, Riegler P, Wirth S, Poehlein A,
Weuster-Botz D, Dürre P (2018) Bacterial anaerobic synthesis gas (syngas) and CO 2 +H 2
fermentation. Adv Appl Microbiol 103:143–221
9. Munasinghe PC, Khanal SK (2010) Biomass-derived syngas fermentation into biofuels:
opportunities and challenges. Bioresour Technol 101:5013–5022
10. Köpke M, Held C, Hujer S, Liesegang H, Wiezer A, Wollherr A, Ehrenreich A, Liebl W,
Gottschalk G, Dürre P (2010) Clostridium ljungdahlii represents a microbial production
platform based on syngas. Proc Natl Acad Sci USA 107:13087–13092
11. Dürre P, Eikmanns BJ (2015) C1-carbon sources for chemical and fuel production by
microbial gas fermentation. Curr Opin Biotechnol 35:63–72
12. Humphreys CM, Minton NP (2018) Advances in metabolic engineering in the microbial
production of fuels and chemicals from C1 gas. Curr Opin Biotechnol 50:174–181
13. Müller V (2019) New horizons in acetogenic conversion of one-carbon substrates and
biological hydrogen storage. Trends Biotechnol 37:1344–1354
14. Schwarz FM, Schuchmann K, Müller V (2018) Hydrogenation of CO 2 at ambient pressure
catalyzed by a highly active thermostable biocatalyst. Biotechnol Biofuels 11:237
15. Schuchmann K, Müller V (2013) Direct and reversible hydrogenation of CO 2 to formate by
a bacterial carbon dioxide reductase. Science 342:1382–1385
16. Müller V, Inkamp F, Rauwolf A, Küsel K, Drake HL (2004) Molecular and cellular biology
of acetogenic bacteria. In: Nakano M, Zuber P (eds) Strict and facultative anaerobes: medical
and environmental aspects. Horizon Scientific Press, Norfolk, pp 251–281
17. Poehlein A, Cebulla M, Ilg MM, Bengelsdorf FR, Schiel-Bengelsdorf B, Whited G,
Andreesen JR, Gottschalk G, Daniel R, Dürre P (2015) The complete genome sequence of
Clostridium aceticum: a missing link between Rnf- and cytochrome-containing autotrophic
acetogens. mBio 6:e01168–01115
18. Schuchmann K, Müller V (2014) Autotrophy at the thermodynamic limit of life: a model for
energy conservation in acetogenic bacteria. Nat Rev Microbiol 12:809–821
19. Wang S, Huang H, Kahnt J, Müller AP, Köpke M, Thauer RK (2013) NADP-specific
electron-bifurcating [FeFe]-hydrogenase in a functional complex with formate dehydrogenase in Clostridium autoethanogenum grown on CO. J Bacteriol 195:4373–4386
20. Yamamoto I, Saiki T, Liu SM, Ljungdahl LG (1983) Purification and properties of
NADP-dependent formate dehydrogenase from Clostridium thermoaceticum, a
tungsten-selenium-iron protein. J Biol Chem 258:1826–1832
21. Maia LB, Moura JJ, Moura I (2015) Molybdenum and tungsten-dependent formate
dehydrogenases. J Biol Inorg Chem 20:287–309
22. O’Brien WE, Brewer JM, Ljungdahl LG (1973) Purification and characterization of
thermostable 5,10-methylenetetrahydrofolate dehydrogenase from Clostridium thermoaceticum. J Biol Chem 248:403–408
23. Ragsdale SW, Ljungdahl LG (1984) Purification and properties of NAD-dependent
5,10-methylenetetrahydrofolate dehydrogenase from Acetobacterium woodii. J Biol Chem
259:3499–3503
24. Moore MR, O’Brien WE, Ljungdahl LG (1974) Purification and characterization of
nicotinamide adenine dinucleotide-dependent methylenetetrahydrofolate dehydrogenase
from Clostridium formicoaceticum. J Biol Chem 249:5250–5253
25. Wohlfarth G, Geerligs G, Diekert G (1990) Purification and properties of a
NADH-dependent 5,10-methylenetetrahydrofolate reductase from Peptostreptococcus productus. Eur J Biochem 192:411–417
Acetogenic Bacteria for Biotechnological Applications
125